scholarly journals Serologic and Molecular Detection of Mycoplasma gallisepticum in Layer Flocks in South Marmara Region of Turkey

2021 ◽  
pp. 29-37
Author(s):  
Elçin Günaydın ◽  
Özlem Kardoğan ◽  
Gülşen Goncagül ◽  
Yavuz Çokal
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Preventive Measures ◽  
Mycoplasma Gallisepticum ◽  
Marmara Region ◽  
Time Loss ◽  
Serological Tests ◽  
Primary Screening ◽  
Pooled Samples

Background: Due to the economic impacts of Mycoplasma gallisepticum (MG) infection in poultry, it is essential to have a fast, reliable and accurate diagnostic test to diagnose the infection. Aims: It was aimed to examine the presence of MG in the South Marmara Region of Turkey where extensive commercial layer flocks exist by RPA, ELISA and real-time PCR. Materials and Methods: In the study, 981 sera and 160 tracheal swab samples (20 swabs per each flock) obtained from eight layer flocks were examined for the presence of MG-antibody by RPA, ELISA, and the presence of MG by real-time PCR, respectively. Results: MG-seropositive flock rate was determined to be 100% by RPA. Twenty-three of the RPA positive sera in each flock LA, LB, LC, LD, LF, LG, and 17 RPA positive sera in flock LE (due to 17 positive RPA sera obtained) were examined for the presence of MG antibody by ELISA, and MG-seropositive flock rate was determined to be 87.5%. As a result of the examination of a total of 32 tracheal swab samples (20 swabs perflock/5 swabs=4 pooled samples, 8 flocksX4 pooled samples= 32 samples) for the presence of MG, real-time PCR positive flock rate was found to be 75%. Conclusion: To decide the flock whether it is infected or not and the initiate effective preventive measures against MG infection as soon as possible; serology should be applied simultaneously with bacteriology and/or PCR to prevent time loss due to shortcomings of serological tests used as primary screening test such as cross reactions, sensitivity and specificity problems.

scholarly journals Evaluation of the New BD Max GC Real-Time PCR Assay, Analytically and Clinically as a Supplementary Test for the BD ProbeTec GC Qx Amplified DNA Assay, for Molecular Detection of Neisseria gonorrhoeae

2015 ◽  
Vol 53 (12) ◽  
pp. 3935-3937 ◽  
Author(s):  
Daniel Golparian ◽  
Stina Boräng ◽  
Martin Sundqvist ◽  
Magnus Unemo
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Analytical Sensitivity ◽  
Pcr Assay ◽  
Content Type ◽  
Dna Assay ◽  
Supplementary Test

The new BD Max GC real-time PCR assay showed high clinical and analytical sensitivity and specificity. It can be an effective and accurate supplementary test for the BD ProbeTec GC Qx amplified DNA assay, which had suboptimal specificity, and might also be used for initial detection ofNeisseria gonorrhoeae.

scholarly journals A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks

2013 ◽  
Vol 44 (2) ◽  
pp. 505-510 ◽  
Author(s):  
Aline Padilha Fraga ◽  
Tatiana de Vargas ◽  
Nilo Ikuta ◽  
André Salvador Kazantzi Fonseca ◽  
Álvaro José Celmer ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mycoplasma Gallisepticum ◽  
Clinical Samples ◽  
Mycoplasma Synoviae ◽  
Commercial Poultry

scholarly journals The Light and Shadow of Rapid Serological Tests for SARS-CoV-2 Infection: Results from a Study in a Large Emergency Department

2020 ◽  
Vol 17 (18) ◽  
pp. 6493
Author(s):  
Daniela Loconsole ◽  
Francesca Centrone ◽  
Caterina Morcavallo ◽  
Silvia Campanella ◽  
Anna Sallustio ◽  
...  
Keyword(s):  
Emergency Department ◽  
Real Time ◽  
Real Time Pcr ◽  
Respiratory Symptoms ◽  
Large Scale ◽  
Serological Test ◽  
Serological Tests ◽  
Serological Assay ◽  
Infected People ◽  
Screening Programs

A critical point in the management of the SARS-CoV-2 pandemic is the need to promptly identify the greatest number of infected people and to implement strict public health measures. In this study, the performance of a rapid serological test in a clinical setting was evaluated. Samples from 819 consecutive patients (with or without respiratory symptoms) admitted to a large Emergency Department were tested between 23 March and 21 April 2020. Patient samples were tested in a real-time PCR assay and a serological assay. In total, 148/819 patients (18.1%) tested positive for SARS-CoV-2 by real-time PCR. The serological test revealed that 70/819 patients (8.5%) had anti-SARS-CoV-2 IgM and/or IgG. The prevalence of anti-SARS-CoV-2 antibodies was significantly higher in patients with respiratory symptoms lasting for >7 days than in those with respiratory symptoms lasting for 0–7 days (p < 0.001). The serological assay had an overall sensitivity of 35.1% and an overall specificity of 97.3%. A high negative predictive value (96.7%) was reported for patients without respiratory symptoms. The results confirm that rapid serological assays alone are not sufficient for diagnosis of SARS-CoV-2 infection but can be incorporated into large-scale screening programs during periods in which the virus circulation is low.

Strain differentiating real-time PCR for Mycoplasma gallisepticum live vaccine evaluation studies

2008 ◽  
Vol 129 (1-2) ◽  
pp. 179-187 ◽  
Author(s):  
Ziv Raviv ◽  
Scott A. Callison ◽  
N. Ferguson-Noel ◽  
Stanley H. Kleven
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Evaluation Studies ◽  
Mycoplasma Gallisepticum ◽  
Live Vaccine

Evaluation of nanopore sequencing as a diagnostic tool for the rapid identification of Mycoplasma bovis from individual and pooled respiratory tract samples

2021 ◽  
Author(s):  
Jade Bokma ◽  
Nick Vereecke ◽  
Mathilde L. Pas ◽  
Laurens Chantillon ◽  
Marianne Vahl ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Real Time ◽  
Diagnostic Tool ◽  
Real Time Pcr ◽  
Latent Class ◽  
Rapid Identification ◽  
Mycoplasma Bovis ◽  
Nanopore Sequencing ◽  
Convenience Sample ◽  
Pooled Samples

Rapid identification of Mycoplasma bovis infections in cattle is a key factor to guide antimicrobial therapy and biosecurity measures. Recently, nanopore sequencing became an affordable diagnostic tool for both clinically relevant viruses and bacteria, but the diagnostic accuracy for M. bovis identification is undocumented. Therefore, in this study nanopore sequencing was compared to rapid identification of M. bovis with MALDI-TOF MS (RIMM), and triplex real-time PCR in a Bayesian latent class model (BLCM) for M. bovis in bronchoalveolar lavage fluid (BALf) obtained from calves. In practice, pooling of samples is often used to save money, but the influence on diagnostic accuracy has not been described for M. bovis . Therefore, a convenience sample of 17 pooled samples containing 5 individual BALf samples per farm was analyzed as well. The results of the pooled samples were compared to the individual samples, to determine sensitivity (Se) and specificity (Sp). The BLCM showed a good Se (77.3%; 95% Credible Interval: 57.8%-92.8%) and high Sp (97.4%; 91.5%-99.7%) for nanopore sequencing compared to RIMM (Se: 93.0%; 76.8%-99.5%, Sp: 91.3; 82.5%-97.0%) and real-time PCR (Se: 94.6%; 89.7%-97.7%, Sp: 86.0%; 76.1-93.6%). Se and Sp of pooled analysis for M. bovis were 85.7% (95% confidence interval: 59.8-111.6%) and 90.0% (71.4-108.6%%) for nanopore sequencing and 100% (100%-100%) and 88.9% (68.4-109.4%) for RIMM, respectively. In conclusion, nanopore sequencing is a rapid, reliable tool for the identification of M. bovis . To reduce costs and increase the chance of M. bovis identification, pooling of 5 samples for nanopore sequencing and RIMM is possible.

Rapid molecular detection of extended-spectrum β-lactamase gene variants with a novel ligation-mediated real-time PCR

2012 ◽  
Vol 61 (11) ◽  
pp. 1563-1567 ◽  
Author(s):  
Roel Nijhuis ◽  
Anton van Zwet ◽  
James Cohen Stuart ◽  
Thijs Weijers ◽  
Paul Savelkoul
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Gene Variants ◽  
Extended Spectrum ◽  
Lactamase Gene

scholarly journals On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

10.3791/56891 ◽  
2018 ◽  
Author(s):  
Joseph B. DeShields ◽  
Rachel A. Bomberger ◽  
James W. Woodhall ◽  
David L. Wheeler ◽  
Natalia Moroz ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection
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