Trichomonas vaginalis ribosomal DNA: analysis of the intergenic region and mapping of the transcription start point

2004 ◽  
Vol 137 (1) ◽  
pp. 175-179 ◽  
Author(s):  
Imelda López-Villaseñor ◽  
Ana Paulina Contreras ◽  
Lorena López-Griego ◽  
Elizbeth Álvarez-Sánchez ◽  
Roberto Hernández
Keyword(s):  
Ribosomal Dna ◽  
Intergenic Region ◽  
Trichomonas Vaginalis ◽  
Dna Analysis ◽  
Transcription Start Point ◽  
Transcription Start

sae is essential for expression of the staphylococcal adhesins Eap and Emp

Microbiology ◽  
2005 ◽  
Vol 151 (6) ◽  
pp. 1789-1800 ◽  
Author(s):  
Niamh Harraghy ◽  
Jan Kormanec ◽  
Christiane Wolz ◽  
Dagmar Homerova ◽  
Christiane Goerke ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Extracellular Matrix ◽  
Virulence Factor ◽  
Eukaryotic Cells ◽  
Transcription Start Point ◽  
Chronic Infections ◽  
Transcription Start ◽  
Regulatory Mutants ◽  
Global Regulators ◽  
Glucose Analysis

Eap and Emp are two Staphylococcus aureus adhesins initially described as extracellular matrix binding proteins. Eap has since emerged as being important in adherence to and invasion of eukaryotic cells, as well as being described as an immunomodulator and virulence factor in chronic infections. This paper describes the mapping of the transcription start point of the eap and emp promoters. Moreover, using reporter-gene assays and real-time PCR in defined regulatory mutants, environmental conditions and global regulators affecting expression of eap and emp were investigated. Marked differences were found in expression of eap and emp between strain Newman and the 8325 derivatives SH1000 and 8325-4. Moreover, both genes were repressed in the presence of glucose. Analysis of expression of both genes in various regulatory mutants revealed that sarA and agr were involved in their regulation, but the data suggested that there were additional regulators of both genes. In a sae mutant, expression of both genes was severely repressed. sae expression was also reduced in the presence of glucose, suggesting that repression of eap and emp in glucose-containing medium may, in part, be a consequence of a decrease in expression of sae.

scholarly journals Analysis of the genetic polymorphism of Paracoccidioides brasiliensis and Paracoccidioides cerebriformis "Moore" by random amplified polymorphic DNA (RAPD) and 28S ribosomal DNA sequencing: Paracoccidioides cerebriformis revisited

2005 ◽  
Vol 47 (3) ◽  
pp. 119-123 ◽  
Author(s):  
Sarah Desirée Barbosa Cavalcanti ◽  
José Eduardo Levi ◽  
Kátia Cristina Dantas ◽  
José Eduardo Costa Martins
Keyword(s):  
Genetic Polymorphism ◽  
Dna Sequence ◽  
Ribosomal Dna ◽  
Dna Analysis ◽  
Paracoccidioides Brasiliensis ◽  
Random Amplified Polymorphic Dna ◽  
Distinct Species ◽  
De Medicina ◽  
28S Ribosomal Dna ◽  
Mycological Laboratory

Our purpose was to compare the genetic polymorphism of six samples of P. brasiliensis (113, 339, BAT, T1F1, T3B6, T5LN1), with four samples of P. cerebriformis (735, 741, 750, 361) from the Mycological Laboratory of the Instituto de Medicina Tropical de São Paulo, using Random Amplified Polymorphic DNA Analysis (RAPD). RAPD profiles clearly segregated P. brasiliensis and P. cerebriformis isolates. However, the variation on band patterns among P. cerebriformis isolates was high. Sequencing of the 28S rDNA gene showed nucleotide conservancy among P. cerebriformis isolates, providing basis for taxonomical grouping, and disclosing high divergence to P. brasiliensis supporting that they are in fact two distinct species. Moreover, DNA sequence suggests that P. cerebriformis belongs in fact to the Aspergillus genus.

Constitutive expression of cat-86 associated with a change in the transcription start point

Gene ◽  
1991 ◽  
Vol 105 (1) ◽  
pp. 113-117 ◽  
Author(s):  
Nicholas P. Ambulos ◽  
Un Jin Kim ◽  
Elizabeth J. Rogers ◽  
Paul S. Lovett
Keyword(s):  
Constitutive Expression ◽  
Transcription Start Point ◽  
Transcription Start

Localization of a transcription start point within the human Hinf element

Gene ◽  
1989 ◽  
Vol 83 (1) ◽  
pp. 173-179 ◽  
Author(s):  
T.Herbert Manoharan ◽  
T. Somasundaram ◽  
G. Chinnadurai
Keyword(s):  
Transcription Start Point ◽  
Transcription Start

Upstream promoter elements are sufficient for selenocysteine tRNA[Ser]Sec gene transcription and to determine the transcription start point

Gene ◽  
1995 ◽  
Vol 162 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Jin Mo Park ◽  
In Soon Choi ◽  
Sung Goo Kang ◽  
Jae Yung Lee ◽  
Dolph L. Hatfield ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Transcription Start Point ◽  
Transcription Start ◽  
Promoter Elements ◽  
Upstream Promoter

scholarly journals Isolation of Abiotrophia adiacens from a Brain Abscess Which Developed in a Patient after Neurosurgery

1999 ◽  
Vol 37 (3) ◽  
pp. 769-771 ◽  
Author(s):  
C. Biermann ◽  
G. Fries ◽  
P. Jehnichen ◽  
S. Bhakdi ◽  
M. Husmann
Keyword(s):  
Cerebrospinal Fluid ◽  
Brain Abscess ◽  
Ribosomal Dna ◽  
Dna Analysis ◽  
Abscess Drainage ◽  
16S Ribosomal Dna ◽  
Specific Pcr ◽  
Microbiological Methods ◽  
Large Brain ◽  
Pcr Products

We report the case of a patient who developed a large brain abscess after neurosurgery. Cerebrospinal fluid from the abscess drainage yielded Abiotrophia adiacens-specific PCR products and microorganisms that were identified by conventional microbiological methods and by 16S ribosomal DNA analysis as Abiotrophia adiacens, which was formerly classified as a member of nutritionally variant streptococci.

scholarly journals DNA Extraction from Soils: Old Bias for New Microbial Diversity Analysis Methods

2001 ◽  
Vol 67 (5) ◽  
pp. 2354-2359 ◽  
Author(s):  
F. Martin-Laurent ◽  
L. Philippot ◽  
S. Hallet ◽  
R. Chaussod ◽  
J. C. Germon ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Ribosomal Dna ◽  
Dna Analysis ◽  
Restriction Analysis ◽  
Farmyard Manure ◽  
Soil Biodiversity ◽  
Soil Dna ◽  
Recovery Method ◽  
Dna Recovery ◽  
The Impact

ABSTRACT The impact of three different soil DNA extraction methods on bacterial diversity was evaluated using PCR-based 16S ribosomal DNA analysis. DNA extracted directly from three soils showing contrasting physicochemical properties was subjected to amplified ribosomal DNA restriction analysis and ribosomal intergenic spacer analysis (RISA). The obtained RISA patterns revealed clearly that both the phylotype abundance and the composition of the indigenous bacterial community are dependent on the DNA recovery method used. In addition, this effect was also shown in the context of an experimental study aiming to estimate the impact on soil biodiversity of the application of farmyard manure or sewage sludge onto a monoculture of maize for 15 years.

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