Adrenergic receptor characterization of CA1 hippocampal neurons using real time single cell RT-PCR

2005 ◽  
Vol 139 (2) ◽  
pp. 267-276 ◽  
Author(s):  
Kristin L. Hillman ◽  
Chris A. Knudson ◽  
Patrick A. Carr ◽  
Van A. Doze ◽  
James E. Porter
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Adrenergic Receptor ◽  
Hippocampal Neurons ◽  
Rt Pcr

scholarly journals Adrenergic receptor characterization of rat hippocampal neurons using real time single cell RT‐PCR

2007 ◽  
Vol 21 (6) ◽  
Author(s):  
Ke Xu ◽  
Kristin Hillman ◽  
Hana Hammad ◽  
Chris Jurgens ◽  
Brianna Goldenstein ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Adrenergic Receptor ◽  
Hippocampal Neurons ◽  
Rt Pcr

scholarly journals Adrenergic Receptor Characterization of Rat Hippocampal CA3 Pyramidal Neurons Using Real Time Single Cell RT‐PCR

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Hana Hammad ◽  
Kristin Hillman ◽  
James Porter ◽  
Van Doze
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Adrenergic Receptor ◽  
Pyramidal Neurons ◽  
Rt Pcr ◽  
Hippocampal Ca3 ◽  
Ca3 Pyramidal Neurons

scholarly journals Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real-time RT-PCR

2005 ◽  
Vol 208 (12) ◽  
pp. 2389-2398 ◽  
Author(s):  
A. Wagatsuma
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Rt Pcr ◽  
Copy Numbers

Real-time fluorescent quantitative RT-PCR assay for the expression of metallothioneins in rat hippocampal neurons

2004 ◽  
Author(s):  
Hai-Hong Qin ◽  
Fu-Di Wang ◽  
Jun-Sheng Guo ◽  
Hui Shen ◽  
Run-Ping Li
Keyword(s):  
Real Time ◽  
Hippocampal Neurons ◽  
Rt Pcr ◽  
Pcr Assay

Real-time characterization of cytotoxicity using single-cell impedance monitoring

The Analyst ◽  
2012 ◽  
Vol 137 (13) ◽  
pp. 3011 ◽  
Author(s):  
Fareid Asphahani ◽  
Myo Thein ◽  
Kui Wang ◽  
David Wood ◽  
Sau Shun Wong ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Cell Impedance ◽  
Impedance Monitoring

Detection and quantification by real-time RT-PCR of hepatitis A virus from inoculated tap waters, salad vegetables, and soft fruits: Characterization of the method performances

2007 ◽  
Vol 117 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Eric Dubois ◽  
Catherine Hennechart ◽  
Ghislaine Merle ◽  
Christian Burger ◽  
Nadia Hmila ◽  
...  
Keyword(s):  
Real Time ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Rt Pcr ◽  
Salad Vegetables ◽  
Soft Fruits ◽  
Detection And Quantification

scholarly journals Global cDNA Amplification Combined with Real-Time RT–PCR: Accurate Quantification of Multiple Human Potassium Channel Genes at the Single Cell Level

Yeast ◽  
2000 ◽  
Vol 1 (3) ◽  
pp. 201-210 ◽  
Author(s):  
A. Al-Taher ◽  
A. Bashein ◽  
T. Nolan ◽  
M. Hollingsworth ◽  
Brady G.
Keyword(s):  
Potassium Channel ◽  
Single Cell ◽  
Real Time ◽  
Quantitative Pcr ◽  
Single Cells ◽  
Initial Step ◽  
Small Samples ◽  
Rt Pcr ◽  
Real Time Quantitative Pcr ◽  
Global Amplification

We have developed a sensitive quantitative RT–PCR procedure suitable for the analysis of small samples, including single cells, and have used it to measure levels of potassium channel mRNAs in a panel of human tissues and small numbers of cells grown in culture. The method involves an initial global amplification of cDNA derived from all added polyadenylated mRNA followed by quantitative RT–PCR of individual genes using specific primers. In order to facilitate rapid and accurate processing of samples, we have adapted the approach to allow use ofTaqMan™real-time quantitative PCR. We demonstrate that the approach represents a major improvement over existing conventional and real-time quantitative PCR approaches, since it can be applied to samples equivalent to a single cell, is able to accurately measure expression levels equivalent to less than 1/100th copy/cell (one specific cDNA molecule present amongst108total cDNA molecules). Furthermore, since the initial step involves a global amplification of all expressed genes, a permanent cDNA archive is generated from each sample, which can be regenerated indefinitely for further expression analysis.

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