Dynamical properties of myoglobin in an ultraviscous water-glycerol solvent investigated with elastic neutron scattering and FTIR spectroscopy

After reviewing some of the basic measurements that characterize the study of physical properties of matter using neutron scattering and nuclear magnetic resonance (NMR), connections between information obtained in current research on fluid membranes using these two complementary techniques are explored in two major chapters. In the first, the type of information on the structure of fluid membranes obtained from coherent elastic neutron scattering is compared with that from NMR spectral characteristics. Then, the type of information obtained on dynamical properties from NMR relaxation (T1 and T2) measurements is compared with that from quasi-elastic neutron scattering. Examples of such connections are given with an emphasis on relationships between the time and distance scales intrinsic to neutron scattering and NMR.

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Quasi-elastic neutron scattering study on polymorphism of tristearin: relationship between dynamical properties and subcell structures

Journal of Crystal Growth ◽

10.1016/j.jcrysgro.2004.11.243 ◽

2005 ◽

Vol 275 (1-2) ◽

pp. e2187-e2193 ◽

Cited By ~ 4

Author(s):

Chikayo Akita ◽

Tatsuya Kawaguchi ◽

Fumitoshi Kaneko ◽

Osamu Yamamuro ◽

Hiroyuki Akita ◽

...

Keyword(s):

Neutron Scattering ◽

Elastic Neutron ◽

Scattering Study ◽

Dynamical Properties ◽

Elastic Neutron Scattering ◽

Neutron Scattering Study

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Dynamical properties of α-synuclein in soluble and fibrillar forms by Quasi Elastic Neutron Scattering

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/j.bbapap.2014.04.010 ◽

2014 ◽

Vol 1844 (7) ◽

pp. 1307-1316 ◽

Cited By ~ 1

Author(s):

Luc Bousset ◽

Clémence Brewee ◽

Ronald Melki ◽

Federica Migliardo

Keyword(s):

Neutron Scattering ◽

Elastic Neutron ◽

Dynamical Properties ◽

Elastic Neutron Scattering

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Fragility of Bioprotectant Glass-Forming Systems in Extremophiles

Journal of Atomic Molecular and Optical Physics ◽

10.1155/2009/638063 ◽

2009 ◽

Vol 2009 ◽

pp. 1-9

Author(s):

S. Magazù ◽

F. Migliardo

Keyword(s):

Glass Transition ◽

Neutron Scattering ◽

Functional State ◽

Extreme Conditions ◽

Dynamical Transition ◽

Elastic Neutron ◽

Glass Forming ◽

Dynamical Regimes ◽

Dynamical Properties ◽

Elastic Neutron Scattering

A central issue in the adaptation of proteins and enzymes to extreme conditions is the conservation of their functional state, which is characterized by a well-balanced compromise of stability and flexibility. In this review work an overview of elastic neutron scattering (ENS) findings on a class of bioprotectant glass-forming systems, such as trehalose and its homologous (maltose and sucrose) water mixtures, is presented as a function of temperature and concentration. ENS, in fact, allows to determine some remarkable quantities in order to characterize the correlation among dynamical properties, the flexibility and fragility of biomolecules. The experimental results have pointed out a dynamical transition, which shows a crossover in molecular fluctuations between harmonic and anharmonic dynamical regimes. The ENS findings allow to characterize both the trehalose rigidity and flexibility, which are strictly connected to its superior bioprotective effectiveness. In this frame the lowest flexibility and fragility character of trehalose/H2O mixture with respect to maltose and sucrose/H2O mixtures indicate a better attitude to encapsulate biostructures in more rigid and temperature insensitive structures in approaching the glass transition.

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