Osteogenic differentiation of stem cells from human exfoliated deciduous teeth on poly(ε-caprolactone) nanofibers containing strontium phosphate

2015 ◽  
Vol 46 ◽  
pp. 427-434 ◽  
Author(s):  
Wen-Ta Su ◽  
Pai-Shuen Wu ◽  
Te-Yang Huang
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Strontium Phosphate ◽  
Human Exfoliated Deciduous Teeth

scholarly journals Metformin enhances osteogenic differentiation of stem cells from human exfoliated deciduous teeth through AMPK pathway

2020 ◽  
Author(s):  
Xuedan Zhao ◽  
Wenyan Huang ◽  
Janak L Pathak ◽  
Chuandong Zhu ◽  
Yunyang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Osteogenic Differentiation ◽  
Specific Inhibitor ◽  
Deciduous Teeth ◽  
Healthy Children ◽  
Matrix Mineralization ◽  
Ampk Pathway ◽  
Compound C ◽  
Human Exfoliated Deciduous Teeth

Abstract Stem cells from human exfoliated deciduous teeth (SHEDs) are ideal seed cells in bone tissue engineering. As a first-line anti-diabetic drug, metformin has recently been found to promote bone formation. The purpose of this study was to investigate the effect of metformin on osteogenic differentiation of SHEDs and its underlying mechanism. SHEDs were isolated from the dental pulp of deciduous teeth from healthy children aged from 6 to 12, and their surface antigen markers of stem cells were detected by flow cytometry. The effect of metformin (10 - 200 μM) treatment on SHEDs cell viability, proliferation, and osteogenic differentiation was analyzed. The activation of adenosine 5'-monophosphate-activated protein kinase (AMPK) was determined by western blot assay for the AMPK phosphorylated at Thr172 (p-AMPK). SHEDs were confirmed as mesenchymal stem cells (MSCs) based on the expression of characteristic surface antigens. Metformin (10-200 μM) did not affect the viability and proliferation of SHEDs, but significantly increased the expression of osteogenic genes, the activity of alkaline phosphatase, matrix mineralization, and p-AMPK level in SHEDs. Compound C, a specific inhibitor of AMPK pathway, abolished metformin-induced osteogenic differentiation of SHEDs. Moreover, metformin treatment enhanced pro-angiogenic/osteogenic growth factors BMP2 and VEGF but reduced the osteoclastogenic factor RANKL/OPG expression in SHEDs. In conclusion, metformin could induce the osteogenic differentiation of SHEDs by activating the AMPK pathway and regulates the expression of pro-angiogenic/osteogenic growth factors and osteoclastogenic factors in SHEDs. Therefore, SHEDs, combined with metformin possesses therapeutic potential for bone regeneration and bone defect repair.

Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth

2010 ◽  
Vol 340 (2) ◽  
pp. 323-333 ◽  
Author(s):  
Kiranmai Chadipiralla ◽  
Ji Min Yochim ◽  
Bindu Bahuleyan ◽  
Chun-Yuh Charles Huang ◽  
Franklin Garcia-Godoy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth ◽  
Periodontal Ligaments

Effect of surface chemistry‐modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth

2021 ◽  
Author(s):  
Jirasak Jitpibull ◽  
Nathaphon Tangjit ◽  
Surachai Dechkunakorn ◽  
Niwat Anuwongnukroh ◽  
Toemsak Srikhirin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Surface Chemistry ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth ◽  
Effect Of Surface

Effect of lithium chloride on cell proliferation and osteogenic differentiation in stem cells from human exfoliated deciduous teeth

2016 ◽  
Vol 48 (5) ◽  
pp. 425-431 ◽  
Author(s):  
Panarat Rattanawarawipa ◽  
Prasit Pavasant ◽  
Thanaphum Osathanon ◽  
Waleerat Sukarawan
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Osteogenic Differentiation ◽  
Lithium Chloride ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth

Rigid matrix supports osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED)

2012 ◽  
Vol 84 (5) ◽  
pp. 366-370 ◽  
Author(s):  
Sandra Viale-Bouroncle ◽  
Martin Gosau ◽  
Kevin Küpper ◽  
Christoph Möhl ◽  
Gero Brockhoff ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Rigid Matrix ◽  
Human Exfoliated Deciduous Teeth

Effect of Jagged-1 and Dll-1 on osteogenic differentiation by stem cells from human exfoliated deciduous teeth

2016 ◽  
Vol 65 ◽  
pp. 1-8 ◽  
Author(s):  
Waleerat Sukarawan ◽  
Karnnapas Peetiakarawach ◽  
Prasit Pavasant ◽  
Thanaphum Osathanon
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth
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