In Vitro Cultivation, Characterization and Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth on 3D Printed Polylactic Acid Scaffolds

2017 ◽  
Vol 19 (8) ◽  
Author(s):  
Aylin Islam ◽  
Emil Mammadov ◽  
Remziye Kendirci ◽  
Ersin Aytac ◽  
Serap Cetiner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Polylactic Acid ◽  
Deciduous Teeth ◽  
In Vitro Cultivation ◽  
3D Printed ◽  
Human Exfoliated Deciduous Teeth

Stem Cells Derived from Human Exfoliated Deciduous teeth [shed] in Neuronal Disorders: A Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Minu Anoop ◽  
Indrani Datta
Keyword(s):  
Stem Cells ◽  
Neurodegenerative Diseases ◽  
Dental Pulp ◽  
Therapeutic Potential ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Proliferative Potential ◽  
Pulp Tissue ◽  
Human Exfoliated Deciduous Teeth

: Most conventional treatments for neurodegenerative diseases fail due to their focus on neuroprotection rather than neurorestoration. Stem cell‐based therapies are becoming a potential treatment option for neurodegenerative diseases as they can home in, engraft, differentiate and produce factors for CNS recovery. Stem cells derived from human dental pulp tissue differ from other sources of mesenchymal stem cells due to their embryonic neural crest origin and neurotrophic property. These include both dental pulp stem cells [DPSCs] from dental pulp tissues of human permanent teeth and stem cells from human exfoliated deciduous teeth [SHED]. SHED offer many advantages over other types of MSCs such as good proliferative potential, minimal invasive procurement, neuronal differentiation and neurotrophic capacity, and negligible ethical concerns. The therapeutic potential of SHED is attributed to the paracrine action of extracellularly released secreted factors, specifically the secretome, of which exosomes is a key component. SHED and its conditioned media can be effective in neurodegeneration through multiple mechanisms, including cell replacement, paracrine effects, angiogenesis, synaptogenesis, immunomodulation, and apoptosis inhibition, and SHED exosomes offer an ideal refined bed-to-bench formulation in neurodegenerative disorders. However, in spite of these advantages, there are still some limitations of SHED exosome therapy, such as the effectiveness of long-term storage of SHED and their exosomes, the development of a robust GMP-grade manufacturing protocol, optimization of the route of administration, and evaluation of the efficacy and safety in humans. In this review, we have addressed the isolation, collection and properties of SHED along with its therapeutic potential on in vitro and in vivo neuronal disorder models as evident from the published literature.

In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth

2014 ◽  
Vol 59 (10) ◽  
pp. 1013-1023 ◽  
Author(s):  
Mijeong Jeon ◽  
Je Seon Song ◽  
Byung-Jai Choi ◽  
Hyung-Jun Choi ◽  
Dong-Min Shin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth

scholarly journals Effect of dexamethasone as osteogenic supplementation in in vitro osteogenic differentiation of stem cells from human exfoliated deciduous teeth

2021 ◽  
Vol 32 (1) ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Raissa Borges Curtarelli ◽  
Izabella Thaís da Silva ◽  
Gislaine Fongaro ◽  
Cesar Augusto Magalhães Benfatti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Osteogenic Differentiation ◽  
Culture Media ◽  
Deciduous Teeth ◽  
Free Calcium ◽  
Alizarin Red ◽  
Matrix Mineralization ◽  
Media Supplementation

AbstractIn in vitro culture systems, dexamethasone (DEX) has been applied with ascorbic acid (ASC) and β-glycerophosphate (βGLY) as culture media supplementation to induce osteogenic differentiation of mesenchymal stem cells. However, there are some inconsistencies regarding the role of DEX as osteogenic media supplementation. Therefore, this study verified the influence of DEX culture media supplementation on the osteogenic differentiation, especially the capacity to mineralize the extracellular matrix of stem cells from human exfoliated deciduous teeth (SHED). Five groups were established: G1—SHED + Dulbecco’s Modified Eagles’ Medium (DMEM) + fetal bovine serum (FBS); G2—SHED + DMEM + FBS + DEX; G3—SHED + DMEM + FBS + ASC + βGLY; G4—SHED + DMEM + FBS + ASC + βGLY + DEX; G5—MC3T3-E1 + α Minimal Essential Medium (MEM) + FBS + ASC + βGLY. DNA content, alkaline phosphatase (ALP) activity, free calcium quantification in the extracellular medium, and extracellular matrix mineralization quantification through staining with von Kossa, alizarin red, and tetracycline were performed on days 7 and 21. Osteogenic media supplemented with ASC and β-GLY demonstrated similar effects on SHED in the presence or absence of DEX for DNA content (day 21) and capacity to mineralize the extracellular matrix according to alizarin red and tetracycline quantifications (day 21). In addition, the presence of DEX in the osteogenic medium promoted less ALP activity (day 7) and extracellular matrix mineralization according to the von Kossa assay (day 21), and more free calcium quantification at extracellular medium (day 21). In summary, the presence of DEX in the osteogenic media supplementation did not interfere with SHED commitment into mineral matrix depositor cells. We suggest that DEX may be omitted from culture media supplementation for SHED osteogenic differentiation in vitro studies.

scholarly journals Metformin enhances osteogenic differentiation of stem cells from human exfoliated deciduous teeth through AMPK pathway

2020 ◽  
Author(s):  
Xuedan Zhao ◽  
Wenyan Huang ◽  
Janak L Pathak ◽  
Chuandong Zhu ◽  
Yunyang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Osteogenic Differentiation ◽  
Specific Inhibitor ◽  
Deciduous Teeth ◽  
Healthy Children ◽  
Matrix Mineralization ◽  
Ampk Pathway ◽  
Compound C ◽  
Human Exfoliated Deciduous Teeth

Abstract Stem cells from human exfoliated deciduous teeth (SHEDs) are ideal seed cells in bone tissue engineering. As a first-line anti-diabetic drug, metformin has recently been found to promote bone formation. The purpose of this study was to investigate the effect of metformin on osteogenic differentiation of SHEDs and its underlying mechanism. SHEDs were isolated from the dental pulp of deciduous teeth from healthy children aged from 6 to 12, and their surface antigen markers of stem cells were detected by flow cytometry. The effect of metformin (10 - 200 μM) treatment on SHEDs cell viability, proliferation, and osteogenic differentiation was analyzed. The activation of adenosine 5'-monophosphate-activated protein kinase (AMPK) was determined by western blot assay for the AMPK phosphorylated at Thr172 (p-AMPK). SHEDs were confirmed as mesenchymal stem cells (MSCs) based on the expression of characteristic surface antigens. Metformin (10-200 μM) did not affect the viability and proliferation of SHEDs, but significantly increased the expression of osteogenic genes, the activity of alkaline phosphatase, matrix mineralization, and p-AMPK level in SHEDs. Compound C, a specific inhibitor of AMPK pathway, abolished metformin-induced osteogenic differentiation of SHEDs. Moreover, metformin treatment enhanced pro-angiogenic/osteogenic growth factors BMP2 and VEGF but reduced the osteoclastogenic factor RANKL/OPG expression in SHEDs. In conclusion, metformin could induce the osteogenic differentiation of SHEDs by activating the AMPK pathway and regulates the expression of pro-angiogenic/osteogenic growth factors and osteoclastogenic factors in SHEDs. Therefore, SHEDs, combined with metformin possesses therapeutic potential for bone regeneration and bone defect repair.

Comparison of human dental follicle cells (DFCs) and stem cells from human exfoliated deciduous teeth (SHED) after neural differentiation in vitro

2009 ◽  
Vol 14 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Christian Morsczeck ◽  
Florian Völlner ◽  
Michael Saugspier ◽  
Caroline Brandl ◽  
Torsten Eugen Reichert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Differentiation ◽  
Follicle Cells ◽  
Deciduous Teeth ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Human Exfoliated Deciduous Teeth

Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth

2010 ◽  
Vol 340 (2) ◽  
pp. 323-333 ◽  
Author(s):  
Kiranmai Chadipiralla ◽  
Ji Min Yochim ◽  
Bindu Bahuleyan ◽  
Chun-Yuh Charles Huang ◽  
Franklin Garcia-Godoy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth ◽  
Periodontal Ligaments

Effect of surface chemistry‐modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth

2021 ◽  
Author(s):  
Jirasak Jitpibull ◽  
Nathaphon Tangjit ◽  
Surachai Dechkunakorn ◽  
Niwat Anuwongnukroh ◽  
Toemsak Srikhirin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Surface Chemistry ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth ◽  
Effect Of Surface
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