Rigid matrix supports osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED)

2012 ◽  
Vol 84 (5) ◽  
pp. 366-370 ◽  
Author(s):  
Sandra Viale-Bouroncle ◽  
Martin Gosau ◽  
Kevin Küpper ◽  
Christoph Möhl ◽  
Gero Brockhoff ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Rigid Matrix ◽  
Human Exfoliated Deciduous Teeth

scholarly journals Metformin enhances osteogenic differentiation of stem cells from human exfoliated deciduous teeth through AMPK pathway

2020 ◽  
Author(s):  
Xuedan Zhao ◽  
Wenyan Huang ◽  
Janak L Pathak ◽  
Chuandong Zhu ◽  
Yunyang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Osteogenic Differentiation ◽  
Specific Inhibitor ◽  
Deciduous Teeth ◽  
Healthy Children ◽  
Matrix Mineralization ◽  
Ampk Pathway ◽  
Compound C ◽  
Human Exfoliated Deciduous Teeth

Abstract Stem cells from human exfoliated deciduous teeth (SHEDs) are ideal seed cells in bone tissue engineering. As a first-line anti-diabetic drug, metformin has recently been found to promote bone formation. The purpose of this study was to investigate the effect of metformin on osteogenic differentiation of SHEDs and its underlying mechanism. SHEDs were isolated from the dental pulp of deciduous teeth from healthy children aged from 6 to 12, and their surface antigen markers of stem cells were detected by flow cytometry. The effect of metformin (10 - 200 μM) treatment on SHEDs cell viability, proliferation, and osteogenic differentiation was analyzed. The activation of adenosine 5'-monophosphate-activated protein kinase (AMPK) was determined by western blot assay for the AMPK phosphorylated at Thr172 (p-AMPK). SHEDs were confirmed as mesenchymal stem cells (MSCs) based on the expression of characteristic surface antigens. Metformin (10-200 μM) did not affect the viability and proliferation of SHEDs, but significantly increased the expression of osteogenic genes, the activity of alkaline phosphatase, matrix mineralization, and p-AMPK level in SHEDs. Compound C, a specific inhibitor of AMPK pathway, abolished metformin-induced osteogenic differentiation of SHEDs. Moreover, metformin treatment enhanced pro-angiogenic/osteogenic growth factors BMP2 and VEGF but reduced the osteoclastogenic factor RANKL/OPG expression in SHEDs. In conclusion, metformin could induce the osteogenic differentiation of SHEDs by activating the AMPK pathway and regulates the expression of pro-angiogenic/osteogenic growth factors and osteoclastogenic factors in SHEDs. Therefore, SHEDs, combined with metformin possesses therapeutic potential for bone regeneration and bone defect repair.

Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth

2010 ◽  
Vol 340 (2) ◽  
pp. 323-333 ◽  
Author(s):  
Kiranmai Chadipiralla ◽  
Ji Min Yochim ◽  
Bindu Bahuleyan ◽  
Chun-Yuh Charles Huang ◽  
Franklin Garcia-Godoy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth ◽  
Periodontal Ligaments

Effect of surface chemistry‐modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth

2021 ◽  
Author(s):  
Jirasak Jitpibull ◽  
Nathaphon Tangjit ◽  
Surachai Dechkunakorn ◽  
Niwat Anuwongnukroh ◽  
Toemsak Srikhirin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Surface Chemistry ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth ◽  
Effect Of Surface

Effect of lithium chloride on cell proliferation and osteogenic differentiation in stem cells from human exfoliated deciduous teeth

2016 ◽  
Vol 48 (5) ◽  
pp. 425-431 ◽  
Author(s):  
Panarat Rattanawarawipa ◽  
Prasit Pavasant ◽  
Thanaphum Osathanon ◽  
Waleerat Sukarawan
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Osteogenic Differentiation ◽  
Lithium Chloride ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth

Effect of Jagged-1 and Dll-1 on osteogenic differentiation by stem cells from human exfoliated deciduous teeth

2016 ◽  
Vol 65 ◽  
pp. 1-8 ◽  
Author(s):  
Waleerat Sukarawan ◽  
Karnnapas Peetiakarawach ◽  
Prasit Pavasant ◽  
Thanaphum Osathanon
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth

scholarly journals Graphene Oxide Quantum Dots Promote Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth via the Wnt/β-Catenin Signaling Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Xin Yang ◽  
Qi Zhao ◽  
JingWen Chen ◽  
Jiayue Liu ◽  
Jiacheng Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantum Dots ◽  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Deciduous Teeth ◽  
Induction Medium ◽  
Nodule Formation ◽  
Graphene Oxide Quantum Dots ◽  
Human Exfoliated Deciduous Teeth

Graphene oxide quantum dots (GOQDs) are a carbon nanomaterial with broad potential for application in the field of nanomaterial biomedicine. Stem cells from human exfoliated deciduous teeth (SHEDs) play an important role in tissue engineering and regenerative medicine. This study investigated the effects of GOQDs on SHED osteogenic differentiation. GOQDs were synthesized; then, the proliferation of SHEDs incubated in GOQDs at different concentrations was evaluated; and the live cells were observed. We observed that live SHEDs incubated in GOQDs emitted green fluorescence in the absence of chemical dyes, and 1, 10, and 50 μg/mL GOQDs significantly promoted SHED proliferation. Culture with the osteogenic induction medium containing 10 μg/mL GOQDs induced calcium nodule formation, increased alkaline phosphatase (ALP) activity, and upregulated SHED mRNA and protein levels of OCN, RUNX2, COL I, and β-catenin. With the addition of Dickkopf 1 (DKK-1) or β-catenin knockdown, expression levels of the above mRNAs and proteins were decreased in GOQD-treated SHEDs. In summary, at a concentration of 10 μg/mL, GOQDs promote SHED proliferation and osteogenic differentiation via the Wnt/β-catenin signaling pathway. This work provides new ideas and fundamental information on interactions between GOQDs and SHEDs that are relevant for the biomedical engineering field.

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