scholarly journals Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells

2019 ◽  
Vol 101 ◽  
pp. 243-253 ◽  
Author(s):  
Zohreh Bagher ◽  
Zhaleh Atoufi ◽  
Rafieh Alizadeh ◽  
Mohammad Farhadi ◽  
Payam Zarrintaj ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Conductive Hydrogel

Differentiation of Mouse Bone-Marrow Mesenchymal Stem Cells into Motor Neuron Cells in vitro

2016 ◽  
Vol 19 (2) ◽  
pp. 111-116
Author(s):  
Rafal Hussamildeen Abdullah ◽  
◽  
Shahlla Mahdi Salih ◽  
Nahi Yosef Yaseen ◽  
Ahmed Majeed Al-Shammari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Mouse Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Gestational Age-Dependent Increase of Survival Motor Neuron Protein in Umbilical Cord-Derived Mesenchymal Stem Cells

2017 ◽  
Vol 5 ◽  
Author(s):  
Sota Iwatani ◽  
Nur Imma Fatimah Harahap ◽  
Dian Kesumapramudya Nurputra ◽  
Shinya Tairaku ◽  
Akemi Shono ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Umbilical Cord ◽  
Gestational Age ◽  
Survival Motor Neuron ◽  
Survival Motor Neuron Protein ◽  
Age Dependent ◽  
Motor Neuron Protein

scholarly journals Directed Induction of Functional Motor Neuron-Like Cells from Genetically Engineered Human Mesenchymal Stem Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e35244 ◽  
Author(s):  
Hwan-Woo Park ◽  
Jung-Sun Cho ◽  
Chul-Kyu Park ◽  
Sung Jun Jung ◽  
Chang-Hwan Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Human Mesenchymal Stem Cells ◽  
Genetically Engineered

Induction of mice adult bone marrow mesenchymal stem cells into functional motor neuron-like cells

2016 ◽  
Vol 77 ◽  
pp. 129-142 ◽  
Author(s):  
Rafal H. Abdullah ◽  
Nahi Y. Yaseen ◽  
Shahlaa M Salih ◽  
Ahmad Adnan Al-Juboory ◽  
Ayman Hassan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Adult Bone Marrow ◽  
Marrow Mesenchymal Stem Cells ◽  
Adult Bone

Purmorphamine as a Shh Signaling Activator Small Molecule Promotes Motor Neuron Differentiation of Mesenchymal Stem Cells Cultured on Nanofibrous PCL Scaffold

2016 ◽  
Vol 54 (7) ◽  
pp. 5668-5675 ◽  
Author(s):  
Naghmeh Bahrami ◽  
Mohammad Bayat ◽  
Abdolreza Mohamadnia ◽  
Mehrdad Khakbiz ◽  
Meysam Yazdankhah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Small Molecule ◽  
Shh Signaling ◽  
Neuron Differentiation ◽  
Cells Cultured

New methods for inducing the differentiation of amniotic-derived mesenchymal stem cells into motor neuron precursor cells

2013 ◽  
Vol 45 (5) ◽  
pp. 295-305 ◽  
Author(s):  
Wei Hu ◽  
Fang-xia Guan ◽  
Yuan Li ◽  
You-jia Tang ◽  
Feng Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Precursor Cells ◽  
New Methods ◽  
Neuron Precursor

scholarly journals Conditioned medium from human gingival mesenchymal stem cells protects motor-neuron-like NSC-34 cells against scratch-injury-induced cell death

2017 ◽  
Vol 30 (4) ◽  
pp. 383-394 ◽  
Author(s):  
Thangavelu Soundara Rajan ◽  
Francesca Diomede ◽  
Placido Bramanti ◽  
Oriana Trubiani ◽  
Emanuela Mazzon
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Cell Death ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Motor Neuron ◽  
Conditioned Medium ◽  
Caspase 3 ◽  
Tnf Α ◽  
Anti Inflammatory

Neuronal cell death is a normal process during central nervous system (CNS) development and is also involved in the death of motor neurons in diverse spinal motor neuron degenerative diseases. Here, we investigated the neuroprotective effect of secretory factors released from human gingival mesenchymal stem cells (hGMSCs) in mechanically injured murine motor-neuron-like NSC-34 cells. The cells were exposed to scratch injury and the markers for apoptosis and oxidative stress were examined. Immunocytochemistry results showed that proapoptotic markers cleaved caspase-3 and Bax were elevated while anti-apoptotic protein Bcl-2 was suppressed in scratch-injured NSC-34 cells. Oxidative stress markers SOD-1, inducible nitric oxide synthase (iNOS), Cox-2, and proinflammatory cytokine tumor necrosis factor alpha (TNF-α) were activated. Conditioned medium (CM) derived from hGMSCs (hGMSC-CM) significantly blocked the cell death by suppressing SOD-1, iNOS, TNF-α, cleaved caspase-3, and Bax. Bcl-2 and anti-inflammatory cytokine anti-interleukin 10 (IL-10) were increased in hGMSC-CM-treated injured cells. Moreover, hGMSC-CM treatment upregulated neurotrophins anti-brain-derived neurotrophic factor (BDNF) and NT3. Western blot data of hGMSC-CM revealed the presence of neurotrophins nerve growth factor (NGF), NT3, anti-inflammatory cytokines IL-10, and transforming growth factor beta (TGF-β), suggesting their positive role to elicit neuroprotection. Our results propose that hGMSC-CM may serve as a simple and potential autologous therapeutic tool to treat motor neuron injury.

scholarly journals Large- Scale Analysis of MicroRNA Expression in Motor Neuron- like Cells derived from Human Umbilical Cord Blood Mesenchymal Stem Cells

2020 ◽  
Author(s):  
Davood Sanooghi ◽  
Abolfazl Lotfi ◽  
Faezeh Faghihi ◽  
Afzal Karimi ◽  
Zohreh Bagher ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Islet 1

Abstract Background Motor neuron- related disorders such as Spinal Cord Injuries and Amyotrophic Lateral Sclerosis are extremely common around the world. Many efforts have been made to use stem cells to modulate regeneration of spinal cord damages. Human umbilical cord blood mesenchymal stem cells (CB-MSCs) cover a class of cells with self-renewal feature and multilineage differentiation capacity. Retinoic acid(RA) and sonic hedgehog(Shh) are two morphogens responsible in motorneuron commitment during development. This study aims to explore the effect of Shh and RA on differentiation of CB-MSCs into motor neuron- like cells and to determine the related microRNA profile. To do that, human MSCs were isolated and then characterized using flowcytometry. The cells were induced using RA and Shh and the outcomes were assessed by immunocytochemistry, real-time- PCR, and flowcytometry. MicroRNA analysis was performed using Solexa system at three libraries, including Test 1 (with RA and Shh), Test 2 (After removing RA and Shh) and the Control. Results The isolated cells were spindle shape and could express MSC markers confirmed by flowcytometry. The cells could express motorneuron- related markers including Islet-1, Hb-9, SMI-32 and ChAT at the level of mRNA and protein, when treated with RA and Shh. Two weeks after induction, the expression of Neun and Islet-1 declined. The analysis of miRNA sequencing revealed a significant expression of mir-let-7b, mir-137 and mir-324-5p, which were responsible for neuron/motor neuron differentiation and suppression of neural progenitor cell proliferation. Moreover, some novel microRNAs involved in cholinergic, Jak- Stat, Hedgehog and Map kinase signaling pathways were revealed. Conclusion CB-MSC represents a type of cells with convenient accessibility, which can be differentiated into motor neuron- like cells in the presence of RA and Shh. We could also detect the expression of candid microRNAs responsible in motor neuron differentiation and some novel microRNAs involved in cholinergic, Jak- Stat, Hedgehog and Map kinase signaling pathways that must be functionally evaluated in further studies.

scholarly journals In Vitro Assessment of the Gene Expression Level of EZH-2 and P300 During Motor Neuron Differentiation of Human Cord Blood Mesenchymal Stem Cells

2021 ◽  
pp. 1-19
Author(s):  
Mohammad Taghi Jogataei ◽  
◽  
Faezeh Faghihi ◽  
Marjaneh Motaghed ◽  
Abolfazl Lotfi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Motor Neuron ◽  
Cord Blood ◽  
Regulatory Genes ◽  
Human Cord Blood ◽  
Over Expression ◽  
Significant Expression ◽  
Islet 1

Introduction: The dedication of stem cells for dissociation into a specific type of cell requires the over expression of genes related to a particular phenotype and suppression of the other genes. Through imposing corresponding alterations on the genome, the genome modulators such as transcription factors can be regulated by histone-modifying enzymes. Maintenance of the neurogenesis process depend on the function of some of these genes which can regulate shifting of cells from proliferation to differentiation such as Enhancer of zeste homolog 2 (EZH2) known as an evolutionarily conserved gene. Moreover, motor neurons (MN) in spinal cord can be regulated during neuronal differentiation via one of the histone acetyltransferase (P300). Up until now, the mechanism of epigenetic regulation and gene expression underlie transition process of human cord blood mesenchymal stem cells (hCB-MSCs) into MNs has not been clarified very well. Therefore, the aim of this study was to explore the quantitative expression of MN-related genes including ChAT, Islet-1, and Mnx-1 along with two epigenetic regulatory genes P300 and EZH2 involved in neurogenesis during differentiation of hCB-MSCs into MNs, using two morphogens including Sonic hedgehog (Shh) and Retinoic acid (RA) involved in the specification of MNs during the growth of nervous system. Methods: Flow cytometry was done to characterize the cells (hCB-MSCs). The cells were differentiated into MN-like cells according to our previous procedure using RA (0.01mM) and Shh (100ng/ ml) as the inducing morphogens. CB-MSCs with no treatment were assumed as control cells. RT-qPCR and Immunocytochemistry were performed to find the expression of interested genes in this study. Results: The expression of MN-related markers was confirmed at the level of mRNA and protein by induction of differentiation. The results was confirmed by immunocytochemistry showed that a number of cells about 55.33±15.885% and 49.67±13.796% could express Islet-1 and ChAT, respectively. The level of gene expression of Islet-1 and ChAT was significantly increased at the first and second week of exposure, respectively. After two weeks, expression level of P300 and EZH-2 genes was increased remarkably. No significant expression of Mnx-1 was detected when compared with the control sample. Conclusion: In this study MN-related markers, Islet-1 and ChAT, were detected in differentiated cells of hCB-MSCs supporting the potency of cord blood cells in regeneration of MN-related disorders. The over expression of Islet-1, as an early MN marker, in the presence of Shh and RA indicates the supportive effect of these morphogens for the onset of motor neuron generation. We could also detect significant expression of two potent epigenetic regulatory genes involved in neurogenesis, P300 and EZH2 accompanied by ChAT as the mature motor neuron marker at the second week of exposure due to the elimination of Shh and RA at later time of differentiation. To our knowledge, the evaluation of P300 and EZH2 during differentiation of hCB-MSCs into MN-like cells was performed in this study for the first time. However, the assessment of these epigenetic regulatory genes at the level of protein can be suggested to confirm their functional epigenetic modifying effects during motor neuron differentiation.

Sign in / Sign up

Export Citation Format

Share Document