scholarly journals CAPS Acts at a Prefusion Step in Dense-Core Vesicle Exocytosis as a PIP2 Binding Protein

Neuron ◽  
2004 ◽  
Vol 43 (4) ◽  
pp. 551-562 ◽  
Author(s):  
Ruslan N Grishanin ◽  
Judith A Kowalchyk ◽  
Vadim A Klenchin ◽  
Kyougsook Ann ◽  
Cynthia A Earles ◽  
...  
Keyword(s):  
Binding Protein ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals Role of the polybasic sequence in the Doc2α C2B domain in dense-core vesicle exocytosis in PC12 cells

2010 ◽  
pp. no-no ◽  
Author(s):  
Mai Sato ◽  
Yasunori Mori ◽  
Takahide Matsui ◽  
Ryo Aoki ◽  
Manami Oya ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

Synaptotagmin function in dense core vesicle exocytosis studied in cracked PC12 cells

10.1038/nn869 ◽  
2002 ◽  
Vol 5 (7) ◽  
pp. 649-656 ◽  
Author(s):  
Ok-Ho Shin ◽  
Josep Rizo ◽  
Thomas C. Südhof
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals Intracellular calcium dependence of large dense-core vesicle exocytosis in the absence of synaptotagmin I

2001 ◽  
Vol 98 (20) ◽  
pp. 11680-11685 ◽  
Author(s):  
T. Voets ◽  
T. Moser ◽  
P.-E. Lund ◽  
R. H. Chow ◽  
M. Geppert ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Calcium Dependence ◽  
Large Dense Core Vesicle ◽  
Synaptotagmin I ◽  
Vesicle Exocytosis

scholarly journals Examining Synaptotagmin 1 Function in Dense Core Vesicle Exocytosis under Direct Control of Ca2+

2003 ◽  
Vol 122 (3) ◽  
pp. 265-276 ◽  
Author(s):  
Jakob B. Sørensen ◽  
Rafael Fernández-Chacón ◽  
Thomas C. Südhof ◽  
Erwin Neher
Keyword(s):  
Dense Core ◽  
Dense Core Vesicle ◽  
Direct Control ◽  
Capacitance Measurements ◽  
Twofold Increase ◽  
Phospholipid Binding ◽  
Caged Calcium ◽  
Vesicle Exocytosis ◽  
Synaptotagmin 1 ◽  
Mutant Cells

We tested the long-standing hypothesis that synaptotagmin 1 is the Ca2+ sensor for fast neurosecretion by analyzing the intracellular Ca2+ dependence of large dense-core vesicle exocytosis in a mouse strain carrying a mutated synaptotagmin C2A domain. The mutation (R233Q) causes a twofold increase in the KD of Ca2+-dependent phospholipid binding to the double C2A-C2B domain of synaptotagmin. Using photolysis of caged calcium and capacitance measurements we found that secretion from mutant cells had lower secretory rates, longer secretory delays, and a higher intracellular Ca2+-threshold for secretion due to a twofold increase in the apparent KD of the Ca2+ sensor for fast exocytosis. Single amperometric fusion events were unchanged. We conclude that Ca2+-dependent phospholipid binding to synaptotagmin 1 mirrors the intracellular Ca2+ dependence of exocytosis.

Role of Doc2α in dense-core vesicle exocytosis in PC12 cells revealed by live cell imaging

2011 ◽  
Vol 71 ◽  
pp. e213
Author(s):  
Takashi Tsuboi ◽  
Yasunori Mori ◽  
Hideki Matsui ◽  
Ryo Aoki ◽  
Manami Oya ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis
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