scholarly journals Intracellular calcium dependence of large dense-core vesicle exocytosis in the absence of synaptotagmin I

2001 ◽  
Vol 98 (20) ◽  
pp. 11680-11685 ◽  
Author(s):  
T. Voets ◽  
T. Moser ◽  
P.-E. Lund ◽  
R. H. Chow ◽  
M. Geppert ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Calcium Dependence ◽  
Large Dense Core Vesicle ◽  
Synaptotagmin I ◽  
Vesicle Exocytosis

scholarly journals RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells

2004 ◽  
Vol 380 (3) ◽  
pp. 875-879 ◽  
Author(s):  
Mitsunori FUKUDA
Keyword(s):  
Rna Interference ◽  
Pc12 Cells ◽  
Small Interfering Rna ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Secretory Vesicles ◽  
Synaptotagmin I ◽  
Immunoaffinity Purification ◽  
Vesicle Exocytosis ◽  
Interfering Rna

Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Sytisoforms acts as the major Ca2+ sensor for dense-core vesicle exocytosis. In the present study, it has been shown by immunoaffinity purification and immunocytochemistry that Syts I and IX, but not Syt IV, are present on the same secretory vesicles in PC12 cells. Silencing of Syt IX with specific small interfering RNA significantly reduced high KCl-dependent neuropeptide Y secretion from PC12 cells, whereas silencing of Syt I with specific small interfering RNA had no significant effect. The results indicate that Syts I and IX are not functionally equivalent and that Syt IX, and not Syt I, is indispensable for the regulation of Ca2+-dependent dense-core vesicle exocytosis in PC12 cells.

scholarly journals Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Kwun Nok M Man ◽  
Cordelia Imig ◽  
Alexander M Walter ◽  
Paulo S Pinheiro ◽  
David R Stevens ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Synaptic Vesicle ◽  
Chromaffin Cells ◽  
Chromaffin Cell ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Docking ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis

It is currently unknown whether the molecular steps of large dense-core vesicle (LDCV) docking and priming are identical to the corresponding reactions in synaptic vesicle (SV) exocytosis. Munc13s are essential for SV docking and priming, and we systematically analyzed their role in LDCV exocytosis using chromaffin cells lacking individual isoforms. We show that particularly Munc13-2 plays a fundamental role in LDCV exocytosis, but in contrast to synapses lacking Munc13s, the corresponding chromaffin cells do not exhibit a vesicle docking defect. We further demonstrate that ubMunc13-2 and Munc13-1 confer Ca2+-dependent LDCV priming with similar affinities, but distinct kinetics. Using a mathematical model, we identify an early LDCV priming step that is strongly dependent upon Munc13s. Our data demonstrate that the molecular steps of SV and LDCV priming are very similar while SV and LDCV docking mechanisms are distinct.

Large Dense-Core Vesicle Exocytosis in PC12 Cells

Methods ◽  
1998 ◽  
Vol 16 (2) ◽  
pp. 204-208 ◽  
Author(s):  
Vadim A. Klenchin ◽  
Judith A. Kowalchyk ◽  
Thomas F.J. Martin
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals Author response: Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis

2015 ◽  
Author(s):  
Kwun Nok M Man ◽  
Cordelia Imig ◽  
Alexander M Walter ◽  
Paulo S Pinheiro ◽  
David R Stevens ◽  
...  
Keyword(s):  
Chromaffin Cell ◽  
Dense Core ◽  
Author Response ◽  
Dense Core Vesicle ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis
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