Odor representation in the olfactory bulb under different brain states revealed by intrinsic optical signals imaging

Neuroscience ◽  
2013 ◽  
Vol 243 ◽  
pp. 54-63 ◽  
Author(s):  
J. Lang ◽  
A. Li ◽  
W. Luo ◽  
R. Wu ◽  
P. Li ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Optical Signals ◽  
Brain States ◽  
Odor Representation

scholarly journals Active Sampling State Dynamically Enhances Olfactory Bulb Odor Representation

Neuron ◽  
2018 ◽  
Vol 98 (6) ◽  
pp. 1214-1228.e5 ◽  
Author(s):  
Rebecca Jordan ◽  
Izumi Fukunaga ◽  
Mihaly Kollo ◽  
Andreas T. Schaefer
Keyword(s):  
Olfactory Bulb ◽  
Active Sampling ◽  
Odor Representation

scholarly journals Optical Imaging of Odor Preference Memory in the Rat Olfactory Bulb

2002 ◽  
Vol 87 (6) ◽  
pp. 3156-3159 ◽  
Author(s):  
Qi Yuan ◽  
Carolyn W. Harley ◽  
John H. McLean ◽  
Thomas Knöpfel
Keyword(s):  
Olfactory Bulb ◽  
Optical Imaging ◽  
Imaging Techniques ◽  
Training Session ◽  
Preference Learning ◽  
Excitatory Postsynaptic Potential ◽  
Odor Preference ◽  
Optical Signals ◽  
Olfactory Preference ◽  
Rat Pups

Early olfactory preference learning in rat pups occurs when novel odors are paired with reinforcing tactile stimulation that activate the noradrenergic locus coeruleus. Pairing of odor and a noradrenergic agonist in the olfactory bulb is both necessary and sufficient for odor preference learning. This suggests the memory change occurs in the olfactory bulb. Previous electrophysiological experiments demonstrated that odor preference training induces an increase in the field excitatory postsynaptic potential to olfactory nerve input and an alteration, after training, in glomerular [14C]2- deoxyglucose uptake and in single-unit responses of principal cells. We investigate here whether, 24 h after olfactory preference training, there is an alteration in intrinsic optical signals at the glomerular level. Six-day-old rat pups were trained, as previously, for a peppermint odor preference. Trained pups and control littermates were subjected to imaging of odor-induced intrinsic optical signals 1 day after the training session. Trained pups exhibited significantly larger responses to the peppermint compared with untrained littermates previously exposed to the same odor. The response of trained pups to a control odor (amyl acetate) was, however, not significantly different from that of untrained littermates. These observations demonstrate that odor preference memory can be read-out by optical imaging techniques.

scholarly journals Complementary Postsynaptic Activity Patterns Elicited in Olfactory Bulb by Stimulation of Mitral/Tufted and Centrifugal Fiber Inputs to Granule Cells

2007 ◽  
Vol 97 (1) ◽  
pp. 296-306 ◽  
Author(s):  
Nora Laaris ◽  
Adam Puche ◽  
Matthew Ennis
Keyword(s):  
Olfactory Bulb ◽  
Ampa Receptor ◽  
Granule Cell ◽  
Granule Cells ◽  
Activity Patterns ◽  
Evoked Responses ◽  
Field Potentials ◽  
Optical Signals ◽  
Postsynaptic Activity ◽  
Stimulation Of

Main olfactory bulb (MOB) granule cells receive spatially segregated glutamatergic synaptic inputs from the dendrites of mitral/tufted cells as well as from the axons of centrifugal fibers (CFFs) originating in olfactory cortical areas. Dendrodendritic synapses from mitral/tufted cells occur on granule cell distal dendrites in the external plexiform layer (EPL), whereas CFFs preferentially target the somata/proximal dendrites of granule cells in the granule cell layer (GCL). In the present study, tract tracing, and recordings of field potentials and voltage-sensitive dye optical signals were used to map activity patterns elicited by activation of these two inputs to granule cells in mouse olfactory bulb slices. Stimulation of the lateral olfactory tract (LOT) produced a negative field potential in the EPL and a positivity in the GCL. CFF stimulation produced field potentials of opposite polarity in the EPL and GCL to those elicited by LOT. LOT-evoked optical signals appeared in the EPL and spread subsequently to deeper layers, whereas CFF-evoked responses appeared in the GCL and then spread superficially. Evoked responses were reduced by N-methyl-d-aspartate (NMDA) receptor antagonists and completely suppressed by AMPA receptor antagonists. Reduction of extracellular Mg2+ enhanced the strength and spatiotemporal extent of the evoked responses. These and additional findings indicate that LOT- and CFF-evoked field potentials and optical signals reflect postsynaptic activity in granule cells, with moderate NMDA and dominant AMPA receptor components. Taken together, these results demonstrate that LOT and CFF stimulation in MOB slices selectively activate glutamatergic inputs to the distal dendrites versus somata/proximal dendrites of granule cells.

scholarly journals Sensory-Evoked Intrinsic Optical Signals in the Olfactory Bulb Are Coupled to Glutamate Release and Uptake

Neuron ◽  
2006 ◽  
Vol 52 (2) ◽  
pp. 335-345 ◽  
Author(s):  
Hirac Gurden ◽  
Naoshige Uchida ◽  
Zachary F. Mainen
Keyword(s):  
Olfactory Bulb ◽  
Glutamate Release ◽  
Optical Signals ◽  
Sensory Evoked

scholarly journals Spontaneous activity in the piriform cortex extends the dynamic range of cortical odor coding

2017 ◽  
Vol 114 (9) ◽  
pp. 2407-2412 ◽  
Author(s):  
Malinda L. S. Tantirigama ◽  
Helena H.-Y. Huang ◽  
John M. Bekkers
Keyword(s):  
Olfactory Bulb ◽  
Spontaneous Activity ◽  
Dynamic Range ◽  
Piriform Cortex ◽  
Spontaneous Firing ◽  
Odor Coding ◽  
Layer 2 ◽  
Olfactory Stimuli ◽  
External Stimuli ◽  
Odor Representation

Neurons in the neocortex exhibit spontaneous spiking activity in the absence of external stimuli, but the origin and functions of this activity remain uncertain. Here, we show that spontaneous spiking is also prominent in a sensory paleocortex, the primary olfactory (piriform) cortex of mice. In the absence of applied odors, piriform neurons exhibit spontaneous firing at mean rates that vary systematically among neuronal classes. This activity requires the participation of NMDA receptors and is entirely driven by bottom-up spontaneous input from the olfactory bulb. Odor stimulation produces two types of spatially dispersed, odor-distinctive patterns of responses in piriform cortex layer 2 principal cells: Approximately 15% of cells are excited by odor, and another approximately 15% have their spontaneous activity suppressed. Our results show that, by allowing odor-evoked suppression as well as excitation, the responsiveness of piriform neurons is at least twofold less sparse than currently believed. Hence, by enabling bidirectional changes in spiking around an elevated baseline, spontaneous activity in the piriform cortex extends the dynamic range of odor representation and enriches the coding space for the representation of complex olfactory stimuli.

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