Shear stress promotes nitric oxide production in endothelial cells by sub-cellular delocalization of eNOS: A basis for shear stress mediated angiogenesis

Nitric Oxide ◽  
2010 ◽  
Vol 22 (4) ◽  
pp. 304-315 ◽  
Author(s):  
Gopi Krishna Kolluru ◽  
Swaraj Sinha ◽  
Syamantak Majumder ◽  
Ajit Muley ◽  
Jamila H. Siamwala ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals In Silico Modeling of Shear-Stress-Induced Nitric Oxide Production in Endothelial Cells through Systems Biology

2013 ◽  
Vol 104 (10) ◽  
pp. 2295-2306 ◽  
Author(s):  
Andrew Koo ◽  
David Nordsletten ◽  
Renato Umeton ◽  
Beracah Yankama ◽  
Shiva Ayyadurai ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Systems Biology ◽  
In Silico ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
In Silico Modeling

Gene Expression of Endothelial Cells under Pulsatile Non-Reversing vs. Steady Shear Stress; Comparison of Nitric Oxide Production

2008 ◽  
Vol 36 (4) ◽  
pp. 571-579 ◽  
Author(s):  
Andrew Yee ◽  
Kami A. Bosworth ◽  
Daniel E. Conway ◽  
Suzanne G. Eskin ◽  
Larry V. McIntire
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Steady Shear ◽  
Nitric Oxide Production ◽  
Oxide Production

Fluid shear stress-induced nitric oxide production in human cavernosal endothelial cells: inhibition by hyperglycaemia

2006 ◽  
Vol 97 (5) ◽  
pp. 1047-1052 ◽  
Author(s):  
HUNTER WESSELLS ◽  
THOMAS H. TEAL ◽  
KAREN ENGEL ◽  
CHRISTOPHER J. SULLIVAN ◽  
BYRON GALLIS ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Fluid Shear Stress ◽  
Nitric Oxide Production ◽  
Fluid Shear ◽  
Oxide Production

scholarly journals Altered nitric oxide production mediates matrix-specific PAK2 and NF-κB activation by flow

2013 ◽  
Vol 24 (3) ◽  
pp. 398-408 ◽  
Author(s):  
Arif Yurdagul ◽  
Jie Chen ◽  
Steven Daniel Funk ◽  
Patrick Albert ◽  
Christopher G. Kevil ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Protein Kinase ◽  
Membrane Proteins ◽  
Basement Membrane ◽  
Protein Kinase A ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Kinase A

Shear stress generated by distinct blood flow patterns modulates endothelial cell phenotype to spatially restrict atherosclerotic plaque development. Signaling through p21-activated kinase (PAK) mediates several of the deleterious effects of shear stress, including enhanced NF-κB activation and proinflammatory gene expression. Whereas shear stress activates PAK in endothelial cells on a fibronectin matrix, basement membrane proteins limit shear-induced PAK activation and inflammation through a protein kinase A–dependent pathway; however, the mechanisms underlying this regulation were unknown. We show that basement membrane proteins limit membrane recruitment of PAK2, the dominant isoform in endothelial cells, by blocking its interaction with the adaptor protein Nck. This uncoupling response requires protein kinase A–dependent nitric oxide production and subsequent PAK2 phosphorylation on Ser-20 in the Nck-binding domain. Of importance, shear stress does not stimulate nitric oxide production in endothelial cells on fibronectin, resulting in enhanced PAK activation, NF-κB phosphorylation, ICAM-1 expression, and monocyte adhesion. These data demonstrate that differential flow–induced nitric oxide production regulates matrix-specific PAK signaling and describe a novel mechanism of nitric oxide–dependent NF-κB inhibition.

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