Polypyrrole-BaFe2O4 sensing layer for detection of strontium ion in aqueous solution using surface plasmon resonance curves fitting

2021 ◽  
Vol 140 ◽  
pp. 106970
Author(s):  
Amir Reza Sadrolhosseini ◽  
Suhaidi Shafie ◽  
Hassan Soleimani ◽  
Mohd. Adzir Mahdi
2012 ◽  
Author(s):  
Amir R. Sadrolhosseini ◽  
A. S. M. Noor ◽  
Mohd Maarof Moksin ◽  
Mahnaz M. Abdi ◽  
Hassan Soleimani ◽  
...  

2017 ◽  
Vol 10 (02) ◽  
pp. 1650040 ◽  
Author(s):  
Tao Ma ◽  
Xiaoxia Chen ◽  
Qing Peng ◽  
Pengfei Zhang ◽  
Yonghong He

Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR) sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis. These operations are all performed under flow condition in the fluidic system, and have the advantages of easy implementation, less time consuming, and low cost, because the reagents and devices used in the operations are routinely applied in most laboratories. In this study, the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.


Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1092
Author(s):  
Nilay Bereli ◽  
Monireh Bakhshpour ◽  
Aykut Arif Topcu ◽  
Adil Denizli

In this work, a surface plasmon resonance (SPR) based immunosensor was prepared by the immobilization of the amine-functionalized gold nanoparticles (N-AuNPs) on the sensing surface to sense immunoglobulin M (IgM) antibodies in the aqueous solution and artificial plasma. The characterization studies of SPR based immunosensor for IgM detection were performed with scanning electron microscope (SEM), contact angle measurements, and ellipsometry. Kinetic studies for the IgM immunosensor were carried out in the range of 1.0 to 200 ng/mL IgM concentrations in an aqueous solution. The total IgM analysis time including adsorption, desorption, and regeneration cycles was nearly 10 min for the prepared immunosensor. The limit of detection (LOD) and limit of quantification (LOQ) were found as 0.08 and 0.26 ng/mL, respectively. The reusability of the proposed immunosensor was tested with 6 consecutive adsorption-desorption, and regeneration cycles. Also, enzyme-linked immunosorbent assay (ELISA) method was utilized in the validation of the immunosensor.


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