Surface Plasmon Resonance-Based Immunosensor for Igm detection with Gold Nanoparticles

In this work, a surface plasmon resonance (SPR) based immunosensor was prepared by the immobilization of the amine-functionalized gold nanoparticles (N-AuNPs) on the sensing surface to sense immunoglobulin M (IgM) antibodies in the aqueous solution and artificial plasma. The characterization studies of SPR based immunosensor for IgM detection were performed with scanning electron microscope (SEM), contact angle measurements, and ellipsometry. Kinetic studies for the IgM immunosensor were carried out in the range of 1.0 to 200 ng/mL IgM concentrations in an aqueous solution. The total IgM analysis time including adsorption, desorption, and regeneration cycles was nearly 10 min for the prepared immunosensor. The limit of detection (LOD) and limit of quantification (LOQ) were found as 0.08 and 0.26 ng/mL, respectively. The reusability of the proposed immunosensor was tested with 6 consecutive adsorption-desorption, and regeneration cycles. Also, enzyme-linked immunosorbent assay (ELISA) method was utilized in the validation of the immunosensor.

Ionospheric disturbance caused by artificial plasma clouds under different release conditions

The generation and evolution of artificial plasma clouds is a complicated process that is strongly dependent on the background environment and release conditions. In this paper, based on a three-dimensional two-species fluid model, the evolution characteristics of artificial plasma clouds under various release conditions were analyzed numerically. In particular, the effect of ionospheric density gradient and ambient horizontal wind field was taken into account in our simulation. The results show that an asymmetric plasma cloud structure occurs in the vertical direction when a nonuniform ionosphere is assumed. The density, volume, and expansion velocity of the artificial plasma cloud vary with the release altitude, mass, and initial ionization rate. The initial release velocity can change the cloud's movement and overall distribution. With an initial velocity perpendicular to the magnetic field, an O+ density cavity and two bumps exist. When there is an initial velocity parallel to the magnetic field, the generated plasma cloud is bulb-shaped, and only one O+ density cavity and one density bump are created. Compared to the cesium case, barium clouds expand more rapidly. Moreover, Cs+ clouds have a higher density than Ba+ clouds, and the snowplow effect of Cs+ is also stronger.

Selected Physicochemical Properties of Diamond Like Carbon (DLC) Coating on Ti-13Nb-13Zr Alloy Used for Blood Contacting Implants

Despite high interest in the issues of hemocompatibility of titanium implants, particularly those made of the Ti-13Nb-13Zr alloy, the applied methods of surface modification still do not always guarantee the physicochemical properties required for their safe operation. The factors that reduce the efficiency of the application of titanium alloys in the treatment of conditions of the cardiovascular system include blood coagulation and fibrous proliferation within the vessel’s internal walls. They result from their surfaces’ physicochemical properties not being fully adapted to the specifics of the circulatory system. Until now, the generation and development mechanics of these adverse processes are not fully known. Thus, the fundamental problem in this work is to determine the correlation between the physicochemical properties of the diamond like carbon (DLC) coating (shaped by the technological conditions of the process) applied onto the Ti-13Nb-13Zr alloy designed for contact with blood and its hemocompatibility. In the paper, microscopic metallographic, surface roughness, wettability, free surface energy, hardness, coating adhesion to the substrate, impendence, and potentiodynamic studies in artificial plasma were carried out. The surface layer with the DLC coating ensures the required surface roughness and hydrophobic character and sufficient pitting corrosion resistance in artificial plasma. On the other hand, the proposed CrN interlayer results in better adhesion of the coating to the Ti-13Nb-13Zr alloy. This type of coating is an alternative to the modification of titanium alloy surfaces using various elements to improve the blood environment’s hemocompatibility.

Biomedical Applications of Hemicellulose-Based Hydrogels

Background: Hydrogel has a three-dimensional network structure that is able to absorb a large amount of water/liquid and maintain its original structure. Hemicellulose (HC) is the second most abundant polysaccharide after cellulose in plants and a heterogeneous polysaccharide consisting of various saccharide units. The unique physical and chemical properties of hemicellulose make it a promising material for hydrogels. Methods: This review first summarizes the three research hotspots on the hemicellulose-based hydrogels: intelligence, biodegradability and biocompatibility. It also overviews the progress in the fabrication and applications of hemicellulose hydrogels in the drug delivery system and tissue engineering (articular cartilage, cell immobilization, and wound dressing). Results: Hemicellulose-based hydrogels have many unique properties, such as stimuliresponsibility, biodegradability and biocompatibility. Interpenetrating networking can endow appropriate mechanical properties to hydrogels. These properties make the hemicellulose-based hydrogels promising materials in biomedical applications such as drug delivery systems and tissue engineering (articular cartilage, cell immobilization, and wound dressing). Conclusion: Hydrogels have been widely used in biomedicine and tissue engineering areas, such as tissue fillers, drug release agents, enzyme encapsulation, protein electrophoresis, contact lenses, artificial plasma, artificial skin, and tissue engineering scaffold materials. This article reviews the recent progress in the fabrication and applications of hemicellulose-based hydrogels in the biomedical field.

Development of Artificial Plasma Membranes Derived Nanovesicles Suitable for Drugs Encapsulation

Extracellular vesicles (EVs) are considered as promising nanoparticle theranostic tools in many pathological contexts. The increasing clinical employment of therapeutic nanoparticles is contributing to the development of a new research area related to the design of artificial EVs. To this aim, different approaches have been described to develop mimetic biologically functional nanovescicles. In this paper, we suggest a simplified procedure to generate plasma membrane-derived nanovesicles with the possibility to efficiently encapsulate different drugs during their spontaneously assembly. After physical and molecular characterization by Tunable Resistive Pulse Sensing (TRPS) technology, transmission electron microscopy, and flow cytometry, as a proof of principle, we have loaded into mimetic EVs the isoquinoline alkaloid Berberine chloride and the chemotherapy compounds Temozolomide or Givinostat. We demonstrated the fully functionality of these nanoparticles in drug encapsulation and cell delivery, showing, in particular, a similar cytotoxic effect of direct cell culture administration of the anticancer drugs. In conclusion, we have documented the possibility to easily generate scalable nanovesicles with specific therapeutic cargo modifications useful in different drug delivery contexts.

Modern heating facility for research into the mid-latitude ionosphere

The development of new devices for research in physics of the upper atmosphere and near-Earth space, which can be used to carry out controlled experiments on the modification of the ionosphere by powerful short-wave radiation, is an urgent task of modern solar-terrestrial physics, space weather, operation of satellite constellations in near-Earth space, radio communications, and radar. The paper describes a modern heating facility, created within the framework of the National Heliogeophysical Complex of the Russian Academy of Sciences. We review the tasks facing the heater, discuss its main technical characteristics, and describe the capability of the observational infrastructure surrounding the heating facility. The paper justifies the long-term benefits of the development of a heating facility at middle latitudes of Eastern Siberia, which can radiate in a frequency range 2.5–6.0 MHz with an effective power of the order of several hundred megawatts. It is important that the heater will be surrounded by such multifunctional instruments as the modern incoherent scatter radar, mesostratospheric lidar, observational systems that can provide a wide range of possibilities for diagnosing artificial plasma disturbances and artificial airglow structures.

Modern heating facility for research into the mid-latitude ionosphere

The development of new devices for research in physics of the upper atmosphere and near-Earth space, which can be used to carry out controlled experiments on the modification of the ionosphere by powerful short-wave radiation, is an urgent task of modern solar-terrestrial physics, space weather, operation of satellite constellations in near-Earth space, radio communications, and radar. The paper describes a modern heating facility, created within the framework of the National Heliogeophysical Complex of the Russian Academy of Sciences. We review the tasks facing the heater, discuss its main technical characteristics, and describe the capability of the observational infrastructure surrounding the heating facility. The paper justifies the long-term benefits of the development of a heating facility at middle latitudes of Eastern Siberia, which can radiate in a frequency range 2.5–6.0 MHz with an effective power of the order of several hundred megawatts. It is important that the heater will be surrounded by such multifunctional instruments as the modern incoherent scatter radar, mesostratospheric lidar, observational systems that can provide a wide range of possibilities for diagnosing artificial plasma disturbances and artificial airglow structures.