Expression of alternative oxidase inhibits programmed cell death-like phenomenon in bloodstream form of Trypanosoma brucei rhodesiense

2005 ◽  
Vol 54 (4) ◽  
pp. 243-251 ◽  
Author(s):  
Akiko Tsuda ◽  
William H. Witola ◽  
Kazuhiko Ohashi ◽  
Misao Onuma
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Trypanosoma Brucei ◽  
Alternative Oxidase ◽  
Bloodstream Form ◽  
Trypanosoma Brucei Rhodesiense

Prostaglandin D2 induces programmed cell death in Trypanosoma brucei bloodstream form

2005 ◽  
Vol 12 (4) ◽  
pp. 335-346 ◽  
Author(s):  
K Figarella ◽  
M Rawer ◽  
N L Uzcategui ◽  
B K Kubata ◽  
K Lauber ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Trypanosoma Brucei ◽  
Bloodstream Form ◽  
Prostaglandin D2

scholarly journals Persistent ER Stress Induces the Spliced Leader RNA Silencing Pathway (SLS), Leading to Programmed Cell Death in Trypanosoma brucei

2010 ◽  
Vol 6 (1) ◽  
pp. e1000731 ◽  
Author(s):  
Hanoch Goldshmidt ◽  
Devorah Matas ◽  
Anat Kabi ◽  
Shai Carmi ◽  
Ronen Hope ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Er Stress ◽  
Trypanosoma Brucei ◽  
Rna Silencing ◽  
Spliced Leader ◽  
Spliced Leader Rna

scholarly journals The inhibition of pyruvate transport across the plasma membrane of the bloodstream form of Trypanosoma brucei and its metabolic implications

1995 ◽  
Vol 312 (2) ◽  
pp. 479-484 ◽  
Author(s):  
E A C Wiemer ◽  
P A M Michels ◽  
F R Opperdoes
Keyword(s):  
Cell Death ◽  
Plasma Membrane ◽  
Trypanosoma Brucei ◽  
Physiological State ◽  
Bloodstream Form ◽  
Eukaryotic Cells ◽  
Monocarboxylate Transporters ◽  
Facilitated Diffusion ◽  
Selective Inhibitors

The pyruvate produced by glycolysis in the bloodstream form of the trypanosome is excreted into the host bloodstream by a facilitated diffusion carrier. The sensitivity of pyruvate transport for alpha-cyano-4-hydroxycinnamate and the compound UK5099 [alpha-cyano-beta-(1-phenylindol-3-yl)acrylate], which are known to be selective inhibitors of pyruvate (monocarboxylate) transporters present in mitochondria and the plasma membrane of eukaryotic cells, was examined. The trypanosomal pyruvate carrier was found to be rather insensitive to inhibition by alpha-cyano-4-hydroxycinnamate (Ki = 17 mM) but could be completely blocked by UK5099 (Ki = 49 microM). Inhibition of pyruvate transport resulted in the retention, and concomitant accumulation, of pyruvate within the trypanosomes, causing acidification of the cytosol and osmotic destabilization of the cells. Our results indicate that this physiological state has serious metabolic consequences and ultimately leads to cell death; thereby identifying the pyruvate carrier as a possible target for chemotherapeutic intervention.

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