Identification and characterization of a novel secreted antigen 1 of Babesia microti and evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test

2011 ◽  
Vol 60 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Yuzi Luo ◽  
Honglin Jia ◽  
M. Alaa Terkawi ◽  
Youn-Kyoung Goo ◽  
Suguru Kawano ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Babesia Microti ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Identification And Characterization ◽  
Potential Use

A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model

2012 ◽  
Vol 130 (2) ◽  
pp. 178-182 ◽  
Author(s):  
Yuzi Luo ◽  
Mohamad Alaa Terkawi ◽  
Honglin Jia ◽  
Gabriel Oluga Aboge ◽  
Youn-Kyoung Goo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Babesia Microti ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hamster Model ◽  
Double Antibody

Production and Characterization of a Monoclonal Antibody to Campylobacter jejuni

2009 ◽  
Vol 72 (4) ◽  
pp. 870-875 ◽  
Author(s):  
S. A. HEO ◽  
R. NANNAPANENI ◽  
M. G. JOHNSON ◽  
J. S. PARK ◽  
K. H. SEO
Keyword(s):  
Monoclonal Antibody ◽  
Campylobacter Jejuni ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Campylobacter Coli ◽  
Carbonate Buffer ◽  
Horse Blood ◽  
Microaerobic Conditions ◽  
Immunoglobulin Subclass

Campylobacter species are a group of spiral-shaped bacteria that can cause disease in humans and animals. We developed a high-affinity monoclonal antibody (MAb) probe that recognizes Campylobacter jejuni cells. Cell suspensions grown under microaerobic conditions at 42°C for 20 h on Bolton agar plates with lysed horse blood were used as live and heat-killed preparations, centrifuged at 8,000 × g for 20 min, and resuspended in carbonate buffer (pH 9.6) for coating on the enzyme-linked immunosorbent assay plates. BALB/c mice were immunized with C. jejuni sonicated cells at 107 CFU/ml to generate MAb-producing hybridoma clones. Of about 500 initial hybridoma clones, MAb 33D2, which reacted with C. jejuni and Campylobacter coli, was selected for further evaluation. MAb 33D2 is in the immunoglobulin subclass G2a and had relatively weaker reactivity with the C. coli strains tested. MAb 33D2 did not show any cross-reactions with the nine non-Campylobacter bacteria tested in the enzyme-linked immunosorbent assay and had a stronger affinity for C. jejuni as live versus heat-killed cells. In Western blot assays, MAb 33D2 recognized two major antigens of 62 and 43 kDa in extracts from C. jejuni cells but only one antigen of 62 kDa in extracts from C. coli cells.

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