Clinical features of septic arthritis with and without Kingella kingae Detection by real-time PCR

Pathology ◽  
2021 ◽  
Vol 53 ◽  
pp. S52
Author(s):  
Abhinav Rajkumar ◽  
Gayana P.S. Gunaratna ◽  
Manori Samarayanake ◽  
Kin-Chuen Leung ◽  
Ameneh Khatami ◽  
...  
Keyword(s):  
Septic Arthritis ◽  
Real Time ◽  
Clinical Features ◽  
Real Time Pcr ◽  
Kingella Kingae

Real-Time PCR Assay for Detection of Kingella kingae in Children

2018 ◽  
Vol 13 (03) ◽  
pp. 216-223
Author(s):  
Theresa Madigan ◽  
Scott Cunningham ◽  
Poornima Ramanan ◽  
Micah Bhatti ◽  
Robin Patel
Keyword(s):  
Synovial Fluid ◽  
Septic Arthritis ◽  
Real Time ◽  
Real Time Pcr ◽  
Limit Of Detection ◽  
Toxin Gene ◽  
Pcr Assay ◽  
Kingella Kingae ◽  
The Real ◽  
Osteoarticular Infections

Background Kingella kingae is a known cause of osteoarticular infections in children younger than 4 years of age, but it is not always recoverable in culture. Molecular methods are increasingly used for diagnosis. Methods To facilitate diagnosis of K. kingae septic arthritis, we developed a real-time polymerase chain reaction (PCR) assay for the detection of K. kingae that targets the repeat-in-toxin gene (rtxB). Results We present three pediatric patients with K. kingae septic arthritis at our institution who were diagnosed using the real-time PCR assay. All underwent arthrotomy with irrigation and debridement and were symptom-free after 3 weeks of therapy with β-lactam antibiotics. Cultures of synovial fluid or tissue grew K. kingae in two of three; K. kingae real-time PCR was positive in all three patients. In addition, 11 cases of K. kingae osteoarticular infection were diagnosed through Mayo Medical Laboratories using this assay. The limit of detection of the real-time PCR assay was 73.7 colony-forming unit (CFU)/µL for tissue and 1.3 CFU/µL for synovial fluid. Conclusions PCR-based detection methods are faster and more sensitive than conventional culture-based methods for the diagnosis of K. kingae osteoarticular infections in children.

scholarly journals Circular RNA sequencing indicates circ-IQGAP2 and circ-ZC3H6 as noninvasive biomarkers of primary Sjögren’s syndrome

Rheumatology ◽  
2020 ◽  
Vol 59 (9) ◽  
pp. 2603-2615 ◽  
Author(s):  
Fengxia Li ◽  
Zhenwei Liu ◽  
Bing Zhang ◽  
Shan Jiang ◽  
Qiongdan Wang ◽  
...  
Keyword(s):  
Receiver Operating Characteristic ◽  
Real Time ◽  
Rna Sequencing ◽  
Clinical Features ◽  
Real Time Pcr ◽  
Operating Characteristic ◽  
Receiver Operating Characteristic Analysis ◽  
Quantitative Real Time Pcr ◽  
Characteristic Analysis ◽  
Noninvasive Biomarkers

Abstract Objectives This study aims to characterize the expression profiles of circRNAs in primary Sjogren’s Syndrome (pSS) and examine the potential of noninvasive circular RNAs (circRNAs) as biomarkers of pSS. Methods We performed RNA sequencing of minor salivary gland (MSG) biopsies from four pSS and four non-pSS individuals (subjects undergoing MSG biopsies but not meeting 2012 or 2016 ACR classification criteria for SS). Differentially expressed circRNAs were identified by DESeq2, and confirmed by quantitative real-time PCR in the MSGs as well as in plasma exosomes in 37 pSS and 14 non-pSS subjects. Discriminatory capacity testing using receiver operating characteristic analysis was used to evaluate the performance of circRNAs as diagnostic biomarkers for pSS. Results Circ-IQGAP2 and circ-ZC3H6 had significantly upregulated expression in the MSGs of pSS patients, and this elevated expression was confirmed by quantitative real-time PCR of plasma exosome RNA. The expression of these circRNAs also showed significant correlation with both clinical features, serum IgG level and MSG focus scores. Receiver operating characteristic analysis showed that the indices comprised of both the two circRNAs and clinical features were better able to distinguish pSS from non-pSS subjects with high mean areas under the curve of 0.93 in the MSGs and 0.92 in the plasma exosomes. Conclusion This study indicated the potential roles of circ-IQGAP2 and circ-ZC3H6 as noninvasive biomarkers for the diagnosis of pSS.

scholarly journals New Real-Time PCR-Based Method for Kingella kingae DNA Detection: Application to Samples Collected from 89 Children with Acute Arthritis

2009 ◽  
Vol 47 (9) ◽  
pp. 3071-3071
Author(s):  
B. Ilharreborde ◽  
P. Bidet ◽  
M. Lorrot ◽  
J. Even ◽  
P. Mariani-Kurkdjian ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Detection ◽  
Kingella Kingae ◽  
Acute Arthritis

scholarly journals Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay

2009 ◽  
Vol 58 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Abdessalam Cherkaoui ◽  
Dimitri Ceroni ◽  
Stéphane Emonet ◽  
Yan Lefevre ◽  
Jacques Schrenzel
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Cell Types ◽  
Cross Reactivity ◽  
Toxin Gene ◽  
Rrna Gene ◽  
Putative Virulence Factor ◽  
Pcr Assay ◽  
Kingella Kingae ◽  
Rtx Toxin

Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens.

scholarly journals New Real-Time PCR-Based Method for Kingella kingae DNA Detection: Application to Samples Collected from 89 Children with Acute Arthritis

2009 ◽  
Vol 47 (6) ◽  
pp. 1837-1841 ◽  
Author(s):  
B. Ilharreborde ◽  
P. Bidet ◽  
M. Lorrot ◽  
J. Even ◽  
P. Mariani-Kurkdjian ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Detection ◽  
Kingella Kingae ◽  
Acute Arthritis

Evaluation of dual target-specific real-time PCR for the detection of Kingella kingae in a Danish paediatric population

2017 ◽  
Vol 50 (3) ◽  
pp. 200-206 ◽  
Author(s):  
Victoria Elizabeth de Knegt ◽  
Gitte Qvist Kristiansen ◽  
Kristian Schønning
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Paediatric Population ◽  
Kingella Kingae ◽  
Dual Target
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