scholarly journals New Real-Time PCR-Based Method for Kingella kingae DNA Detection: Application to Samples Collected from 89 Children with Acute Arthritis

2009 ◽  
Vol 47 (9) ◽  
pp. 3071-3071
Author(s):  
B. Ilharreborde ◽  
P. Bidet ◽  
M. Lorrot ◽  
J. Even ◽  
P. Mariani-Kurkdjian ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Detection ◽  
Kingella Kingae ◽  
Acute Arthritis

scholarly journals New Real-Time PCR-Based Method for Kingella kingae DNA Detection: Application to Samples Collected from 89 Children with Acute Arthritis

2009 ◽  
Vol 47 (6) ◽  
pp. 1837-1841 ◽  
Author(s):  
B. Ilharreborde ◽  
P. Bidet ◽  
M. Lorrot ◽  
J. Even ◽  
P. Mariani-Kurkdjian ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Detection ◽  
Kingella Kingae ◽  
Acute Arthritis

The problem of bovine histophilosis and Histophilus somni DNA detection by real-time PCR

2020 ◽  
Vol 23 (11) ◽  
pp. 28-33
Author(s):  
S.P. Yatsentyuk ◽  
◽  
D.A. Rudnyayev ◽  
Yu.I. Pobolelova ◽  
M.S. Krasnikova ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Detection ◽  
Histophilus Somni

scholarly journals Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay

2009 ◽  
Vol 58 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Abdessalam Cherkaoui ◽  
Dimitri Ceroni ◽  
Stéphane Emonet ◽  
Yan Lefevre ◽  
Jacques Schrenzel
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Cell Types ◽  
Cross Reactivity ◽  
Toxin Gene ◽  
Rrna Gene ◽  
Putative Virulence Factor ◽  
Pcr Assay ◽  
Kingella Kingae ◽  
Rtx Toxin

Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens.

scholarly journals High-throughput HTLV-1 proviral DNA detection system using a nucleic acid extraction robot and real-time PCR detection.

2001 ◽  
Vol 47 (3) ◽  
pp. 378-383 ◽  
Author(s):  
Chieko Matsumoto ◽  
Rieko Shiozawa ◽  
Shigeki Mitsunaga ◽  
Akiko Ichikawa ◽  
Rika Ishiwatari ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Detection System ◽  
Dna Detection ◽  
Pcr Detection ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Proviral Dna

Performance of two real-time PCR assays for hepatitis B virus DNA detection and quantitation

2014 ◽  
Vol 201 ◽  
pp. 24-30 ◽  
Author(s):  
Dramane Kania ◽  
Laure Ottomani ◽  
Nicolas Meda ◽  
Marianne Peries ◽  
Pierre Dujols ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Detection ◽  
Hepatitis B Virus Dna ◽  
B Virus ◽  
Pcr Assays

Evaluation of dual target-specific real-time PCR for the detection of Kingella kingae in a Danish paediatric population

2017 ◽  
Vol 50 (3) ◽  
pp. 200-206 ◽  
Author(s):  
Victoria Elizabeth de Knegt ◽  
Gitte Qvist Kristiansen ◽  
Kristian Schønning
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Paediatric Population ◽  
Kingella Kingae ◽  
Dual Target
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