Evaluation of vitamin D receptor genetic polymorphisms with dental caries and developmental defects of enamel in Brazilian children

Purpose: Recent studies have suggested that disruptions in the RANKL/RANK/OPG system might be involved in enamel conditions. The aim of this study was to test whether genetic polymorphisms in RANK, RANKL and OPG are associated with dental caries, developmental defects of enamel (DDE) and enamel microhardness. Study design: Saliva samples were collected from two subsets for the purpose of genomic DNA extraction. In the first subset, composed of 248 children, dental caries and DDE were evaluated during their clinical examination. In the second subset, composed of 72 children, enamel samples from the buccal surface of primary teeth were used for enamel microhardness analysis. Genetic polymorphisms in RANK, RANKL and OPG were genotyped by real-time polymerase chain reactions in all samples from both populations. The chi-square test was used for dental caries and DDE analysis while, one-way ANOVA with Tukey's post-test was used for microhardness analysis. Hardy-Weinberg equilibrium was also calculated. The established alpha was 5%. Results: Caries experience analysis demonstrated a statistically-significant difference for OPG allele distribution in primary dentition (p=0.033). The studied polymorphisms in RANK, RANKL and OPG were not associated with DDE or enamel microhardness (p>0.05). Conclusion: The genetic polymorphism rs2073618 in OPG is associated with dental caries experience in primary dentition.

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MMP13 Contributes to Dental Caries Associated with Developmental Defects of Enamel

Caries Research ◽

10.1159/000496372 ◽

2019 ◽

Vol 53 (4) ◽

pp. 441-446 ◽

Cited By ~ 6

Author(s):

Katia Regina Vasconcelos ◽

Juliana Arid ◽

Silvane Evangelista ◽

Sara Oliveira ◽

André Luiz Dutra ◽

...

Keyword(s):

Genetic Polymorphism ◽

Dental Caries ◽

Genetic Polymorphisms ◽

Clinical Examination ◽

Genomic Dna ◽

Amazon Region ◽

Caries Experience ◽

Exact Tests ◽

Developmental Defects ◽

Developmental Defects Of Enamel

The aim of this study was to investigate the association between genetic polymorphisms in MMP8, MMP13, and MMP20 with caries experience and developmental defects of enamel (DDE) in children from the Amazon region of Brazil. Den tal caries and DDE data were collected through clinical examination from 216 children. Genomic DNA was extracted from saliva, and genotyping of selected polymorphisms in MMP8 (rs17099443 and rs3765620), MMP13 (rs478927 and rs2252070), and MMP20 (rs1784418) was performed using TaqMan chemistry and endpoint analysis. χ2 or Fisher’s exact tests were used to compare allele and genotype distributions between children with caries experience and caries-free children and between DDE-affected and -unaffected children with an established alpha of 5%. The polymorphism rs478927 in MMP13 was associated with caries experience and DDE (p < 0.05). The analysis performed comparing children with both conditions (caries experience plus DDE) and children with neither of the conditions (caries-free chil dren without DDE) demonstrated that children carrying the MMP13 rs478927 TT genotype were more likely to have concomitant occurrence of these two conditions (OR = 5.8, 95% CI 2.1–15.8; p = 0.0003). In conclusion, the genetic polymorphism rs478927 in MMP13 was associated with caries experience and DDE.

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Vitamin D receptor rs2228570 and rs1544410 genetic polymorphisms frequency in Iraqi thalassemia patients compared to other ethnic populations

Gene Reports ◽

10.1016/j.genrep.2021.101131 ◽

2021 ◽

Vol 23 ◽

pp. 101131

Author(s):

Ali Hafedh Abbas ◽

Dunya Fareed Salloom ◽

Khawla Ibrahim Misha'al ◽

Estabraq Abdulhadi Taqi

Keyword(s):

Vitamin D ◽

Genetic Polymorphisms ◽

Vitamin D Receptor ◽

Ethnic Populations

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Risk of Dental Caries in Primary Teeth with Developmental Defects of Enamel: A Longitudinal Study with a Multilevel Approach

Caries Research ◽

10.1159/000501029 ◽

2019 ◽

Vol 53 (6) ◽

pp. 667-674

Author(s):

Suzane Paixão-Gonçalves ◽

Patrícia Corrêa-Faria ◽

Fernanda Morais Ferreira ◽

Maria Letícia Ramos-Jorge ◽

Saul Martins Paiva ◽

...

Keyword(s):

Dental Caries ◽

Primary Teeth ◽

Primary Dentition ◽

Enamel Hypoplasia ◽

Developmental Defects ◽

Posterior Teeth ◽

Multilevel Approach ◽

Two Stages ◽

Developmental Defects Of Enamel

The risk of dental caries seems to be greater in the presence of developmental defects of enamel (DDE). The aim was to determine whether the presence of DDE in the primary teeth of preschool children increases the risk of dental caries in the primary dentition after a period of approximately 2 years. This study was conducted in two stages: baseline (T0) and follow-up (T1). At T0, examinations were conducted for the diagnosis of enamel defects using the DDE index (FDI, 1992), dental caries, and oral hygiene. The participants were allocated to two groups according to the presence (affected) or absence (unaffected) of DDE. At the second evaluation (T1), examinations were performed for the diagnosis of dental caries. Poisson regression analysis with a multilevel approach was used to determine the association between DDE and dental caries. The two levels of the analysis were tooth and child. Among the 339 children (113 affected and 226 unaffected) examined at baseline (T0), 325 were re-examined at follow-up (T1). According to the multilevel analysis, teeth with enamel hypoplasia had a greater risk of having dental caries (RR: 1.99; 95% CI: 1.19–3.33). The risk of caries was greater on posterior teeth (RR: 2.59; 95% CI: 2.18–3.07) and maxillary teeth (RR: 1.48; 95% CI: 1.26–1.75) that had DDE at T0. On the child level, dental caries at T1 was associated with having dental caries at T0 (RR: 1.38; 95% CI: 1.32–1.46). In conclusion, enamel hypoplasia and previous dental caries are risk factors for carious lesions in the primary dentition.

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