Retraction notice to “High-level expression and purification of recombinant huGM-CSF(9–127)/IL-6(29–184) fusion protein in Escherichia coli” [Protein Express. Purif. 42 (2005) 278–285]

2007 ◽  
Vol 52 (2) ◽  
pp. 486
Author(s):  
Qiang-ming Sun ◽  
Hong-chao Jiang ◽  
Wei-ming Xu ◽  
Xin Liu ◽  
Chang-bai Dai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Expression And Purification ◽  
High Level Expression ◽  
Retraction Notice ◽  
High Level

High Level Expression and Purification of King Cobra Cathelicidin OH-CATH30 in Escherichia coli as a Thioredoxin Fusion Protein

2014 ◽  
Vol 926-930 ◽  
pp. 997-1000
Author(s):  
Tong Yi Sun
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Therapeutic Potential ◽  
Expression System ◽  
Expression And Purification ◽  
High Level Expression ◽  
High Level ◽  
Systemic Infections ◽  
King Cobra

The Cathelicidin OH-CATH30 may have therapeutic potential against the systemic infections. However, it is a great challenge to obtain abundant OH-CATH30 by Escherichia coli expression system. The OH-CATH30 coding sequence was optimized and subcloned into vector pET-32a, allowing the peptide to be expressed as a thioredoxin fusion protein. The highest protein expression level obtained was 100 mg/L of bacterial culture. Before being cleaved with enterokinase, the released recombinant OH-CATH30 exhibited a in vitro strong antibacterial activity against the control strains.

scholarly journals High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli

Molecules ◽  
2018 ◽  
Vol 23 (4) ◽  
pp. 800 ◽  
Author(s):  
Kuang-Ting Cheng ◽  
Chih-Lung Wu ◽  
Bak-Sau Yip ◽  
Hui-Yuan Yu ◽  
Hsi-Tsung Cheng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Peptide ◽  
Expression And Purification ◽  
High Level Expression ◽  
High Level

Elastin-like polypeptide fusions for high-level expression and purification of human IFN-γ in Escherichia coli

2019 ◽  
Vol 585 ◽  
pp. 113401 ◽  
Author(s):  
Reza Heidari-Japelaghi ◽  
Raheem Haddad ◽  
Mostafa Valizadeh ◽  
Ebrahim Dorani-Uliaie ◽  
Mokhtar Jalali-Javaran
Keyword(s):  
Escherichia Coli ◽  
Expression And Purification ◽  
High Level Expression ◽  
Ifn Γ ◽  
High Level

High-level expression and purification of mature HIV-1 protease in Escherichia coli under control of the araBAD promoter

1992 ◽  
Vol 37 (2) ◽  
Author(s):  
Alison Taylor ◽  
DavidP. Brown ◽  
Sunil Kadam ◽  
Mary Maus ◽  
WilliamE. Kohlbrenner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Expression And Purification ◽  
High Level Expression ◽  
High Level ◽  
Hiv 1

High-level expression of tetanus toxin fragment C‒thioredoxin fusion protein in Escherichia coli

2000 ◽  
Vol 31 (2) ◽  
pp. 91 ◽  
Author(s):  
Adriana V. Ribas ◽  
Paulo L. Ho ◽  
Martha M. Tanizaki ◽  
Isaias Raw ◽  
Ana L.T.O. Nascimento
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Tetanus Toxin ◽  
High Level Expression ◽  
High Level
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