Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis

2015 ◽  
Vol 112 ◽  
pp. 15-20 ◽  
Author(s):  
Antje Lindae ◽  
Raphael J. Eberle ◽  
Icaro P. Caruso ◽  
Monika A. Coronado ◽  
Fabio R. de Moraes ◽  
...  
Keyword(s):  
Cold Shock ◽  
Protein A ◽  
Cold Shock Protein ◽  
Corynebacterium Pseudotuberculosis ◽  
Purification And Characterization

scholarly journals Purification and Characterization of a Novel Cold Shock Protein-Like Bacteriocin Synthesized by Bacillus thuringiensis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Tianpei Huang ◽  
Xiaojuan Zhang ◽  
Jieru Pan ◽  
Xiaoyu Su ◽  
Xin Jin ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Cold Shock ◽  
Food Preservation ◽  
Proteinase K ◽  
Cold Shock Protein ◽  
Purification And Characterization ◽  
Large Conformational Change ◽  
Proteinase K Treatment ◽  
Ph Range

Abstract Bacillus thuringiensis (Bt), one of the most successful biopesticides, may expand its potential by producing bacteriocins (thuricins). The aim of this study was to investigate the antimicrobial potential of a novel Bt bacteriocin, thuricin BtCspB, produced by Bt BRC-ZYR2. The results showed that this bacteriocin has a high similarity with cold-shock protein B (CspB). BtCspB lost its activity after proteinase K treatment; however it was active at 60 °C for 30 min and was stable in the pH range 5–7. The partial loss of activity after the treatments of lipase II and catalase were likely due to the change in BtCspB structure and the partial degradation of BtCspB, respectively. The loss of activity at high temperatures and the activity variation at different pHs were not due to degradation or large conformational change. BtCspB did not inhibit four probiotics. It was only active against B. cereus strains 0938 and ATCC 10987 with MIC values of 3.125 μg/mL and 0.781 μg/mL, and MBC values of 12.5 μg/mL and 6.25 μg/mL, respectively. Taken together, these results provide new insights into a novel cold shock protein-like bacteriocin, BtCspB, which displayed promise for its use in food preservation and treatment of B. cereus-associated diseases.

Characterization of Cold-Shock Protein A of Antarctic Streptomyces sp. AA8321

2007 ◽  
Vol 26 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Min-Jung Kim ◽  
Yoo Kyung Lee ◽  
Hong Kum Lee ◽  
Hana Im
Keyword(s):  
Cold Shock ◽  
Protein A ◽  
Cold Shock Protein ◽  
Streptomyces Sp

Dynamical properties of cold shock protein A from Mycobacterium tuberculosis

2010 ◽  
Vol 402 (4) ◽  
pp. 693-698 ◽  
Author(s):  
Gabriella D’Auria ◽  
Carla Esposito ◽  
Lucia Falcigno ◽  
Luisa Calvanese ◽  
Emanuela Iaccarino ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cold Shock ◽  
Protein A ◽  
Cold Shock Protein ◽  
Dynamical Properties

scholarly journals Characterization of an unusual cold shock protein from Staphylococcus aureus

2010 ◽  
Vol 50 (6) ◽  
pp. 519-526 ◽  
Author(s):  
Palas K. Chanda ◽  
Amitava Bandhu ◽  
Biswanath Jana ◽  
Rajkrishna Mondal ◽  
Tridib Ganguly ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cold Shock ◽  
Cold Shock Protein
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