Expression of feruloyl esterase A from Aspergillus terreus and its application in biomass degradation

2015 ◽  
Vol 115 ◽  
pp. 153-157 ◽  
Author(s):  
Shuai-Bing Zhang ◽  
Le Wang ◽  
Yan Liu ◽  
Huan-Chen Zhai ◽  
Jing-Ping Cai ◽  
...  
Author(s):  
Camila L. Corrêa ◽  
Glaucia E. O. Midorikawa ◽  
Edivaldo Ximenes Ferreira Filho ◽  
Eliane Ferreira Noronha ◽  
Gabriel S. C. Alves ◽  
...  

2016 ◽  
Vol 100 (21) ◽  
pp. 9133-9144 ◽  
Author(s):  
Gabriela Leal Vitcosque ◽  
Liliane Fraga Costa Ribeiro ◽  
Rosymar Coutinho de Lucas ◽  
Tony Marcio da Silva ◽  
Lucas Ferreira Ribeiro ◽  
...  

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1229
Author(s):  
Xiaoli Duan ◽  
Yiwei Dai ◽  
Tao Zhang

Feruloyl esterase (FAE; EC 3.1.1.73) catalyzes the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl group in an esterified sugar to assist in waste biomass degradation or to release ferulic acid (FA). An FAE-producing strain was isolated from humus soil samples and identified as Bacillus pumilus SK52.001. The BpFAE gene from B. pumilus SK52.001 was speculated and heterogeneously expressed in Bacillus subtilis WB800 for the first time. The enzyme exists as a monomer with 303 amino acids and a molecular mass of 33.6 kDa. Its specific activity was 377.9 ± 10.3 U/ (mg protein), using methyl ferulate as a substrate. It displays an optimal alkaline pH of 9.0, an optimal temperature of 50 °C, and half-lives of 1434, 327, 235, and 68 min at 50, 55, 60, and 65 °C, respectively. Moreover, the purified BpFAE released 4.98% FA of the alkali-acidic extractable FA from de-starched wheat bran (DSWB). When the DSWB was enzymatically degraded by the synergistic effect of the BpFAE and commercial xylanase, the FA amount reached 49.47%. It suggested that the alkaline BpFAE from B. pumilus SK52.001, which was heterologously expressed in B. subtilis WB800, possesses great potential for biomass degradation and achieving high-added value FA production from food by-products.


Author(s):  
Ana Daniela Vega-Rodríguez ◽  
Mariana Antonieta Armendáriz-Ruiz ◽  
Daniel Alberto Grajales-Hernández ◽  
Jorge Alberto Rodríguez-González ◽  
Alí Asaff-Torres ◽  
...  

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
WY Liao ◽  
CN Shen ◽  
LH Lin ◽  
YL Yang ◽  
HY Han ◽  
...  

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