Screening novel β-galactosidases from a sequence-based metagenome and characterization of an alkaline β-galactosidase for the enzymatic synthesis of galactooligosaccharides

2019 ◽  
Vol 155 ◽  
pp. 104-111 ◽  
Author(s):  
Pei Liu ◽  
Wei Wang ◽  
Jian Zhao ◽  
Dongzhi Wei
Keyword(s):  
Enzymatic Synthesis

Characterization of an isozyme of S-adenosylmethionine synthetase from rat liver

1984 ◽  
Vol 62 (5) ◽  
pp. 276-279 ◽  
Author(s):  
C. H. Lin ◽  
W. Chung ◽  
K. P. Strickland ◽  
A. J. Hudson
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Amino Acid ◽  
Enzymatic Synthesis ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Aromatic Amino Acids ◽  
Adenosylmethionine Synthetase ◽  
Cellulose Column

An isozyme of S-adenosylmethionine synthetase has been purified to homogeneity by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and gel filtration on a Sephadex G-200 column. The purified enzyme is very unstable and has a molecular weight of 120 000 consisting of two identical subunits. Amino acid analysis on the purified enzyme showed glycine, glutamate, and aspartate to be the most abundant and the aromatic amino acids to be the least abundant. It possesses tripolyphosphatase activity which can be stimulated five to six times by S-adenosylmethionine (20–40 μM). The findings support the conclusion that an enzyme-bound tripolyphosphate is an obligatory intermediate in the enzymatic synthesis of S-adenosylmethionine from ATP and methionine.

scholarly journals Enzymatic synthesis and characterization of muconic acid‐based unsaturated polymer systems

2020 ◽  
Author(s):  
Dina Maniar ◽  
Csaba Fodor ◽  
Indra Karno Adi ◽  
Albert JJ Woortman ◽  
Jur Dijken ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Synthesis And Characterization ◽  
Muconic Acid ◽  
Polymer Systems ◽  
Unsaturated Polymer

scholarly journals Enzymatic Synthesis and Characterization of Thermosensitive Polyester with Pendent Ketoprofen

Polymers ◽  
2013 ◽  
Vol 5 (3) ◽  
pp. 1158-1168 ◽  
Author(s):  
Wan-Xia Wu ◽  
Hai-Yang Wang ◽  
Na Wang ◽  
Wei-Wei Zhang ◽  
Han Lai ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Synthesis And Characterization
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