scholarly journals Purification and characterization of native human elongation factor 2

2019 ◽  
Vol 158 ◽  
pp. 15-19
Author(s):  
Rasmus Kock Flygaard ◽  
Beatrice Malacrida ◽  
Patrick Kiely ◽  
Lasse Bohl Jenner
Keyword(s):  
Elongation Factor ◽  
Purification And Characterization ◽  
Elongation Factor 2 ◽  
Human Elongation Factor

scholarly journals Purification and characterization of tagless recombinant human elongation factor 2 kinase (eEF-2K) expressed in Escherichia coli

2011 ◽  
Vol 79 (2) ◽  
pp. 237-244 ◽  
Author(s):  
Olga Abramczyk ◽  
Clint D.J. Tavares ◽  
Ashwini K. Devkota ◽  
Alexey G. Ryazanov ◽  
Benjamin E. Turk ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Elongation Factor ◽  
Purification And Characterization ◽  
Elongation Factor 2 ◽  
Human Elongation Factor

scholarly journals Purification and characterization of the protein kinase eEF-2 isolated from rat liver cells.

1994 ◽  
Vol 41 (4) ◽  
pp. 421-427
Author(s):  
A Gajko ◽  
W Gałasiński ◽  
A Gindzieński
Keyword(s):  
Protein Kinase ◽  
Rat Liver ◽  
Elongation Factor ◽  
Liver Cells ◽  
Relative Molecular Mass ◽  
Purification And Characterization ◽  
Elongation Factor 2 ◽  
Inactive Form ◽  
Rat Liver Cells

The elongation factor 2 (eEF-2) protein kinase was isolated from rat liver cells, purified and partly characterized. It was found that the enzyme exists in an inactive form in the homogenate of rat liver. The active fraction of kinase eEF-2 was obtained after removal of the inhibitory substance by hydroxyapatite column chromatography. The purified enzyme is an electrophoretically homogeneous protein with relative molecular mass of approximately 90,000 and isoelectric point, pI = 5.9. The enzyme specifically phosphorylates the elongation factor eEF-2 in the presence of calmodulin and Ca2+.

scholarly journals Saccharomyces cerevisiae elongation factor 2. Genetic cloning, characterization of expression, and G-domain modeling.

1992 ◽  
Vol 267 (2) ◽  
pp. 1190-1197 ◽  
Author(s):  
J P Perentesis ◽  
L D Phan ◽  
W B Gleason ◽  
D C LaPorte ◽  
D M Livingston ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Elongation Factor ◽  
Domain Modeling ◽  
Elongation Factor 2

scholarly journals Chromosomal assignment of the gene for human elongation factor 2.

1984 ◽  
Vol 81 (10) ◽  
pp. 3158-3162 ◽  
Author(s):  
Y. Kaneda ◽  
M. C. Yoshida ◽  
K. Kohno ◽  
T. Uchida ◽  
Y. Okada
Keyword(s):  
Elongation Factor ◽  
Chromosomal Assignment ◽  
Elongation Factor 2 ◽  
Human Elongation Factor

Construction of a Plasmid Containing the Complete Coding Region of Human Elongation Factor 2

1992 ◽  
Vol 373 (1) ◽  
pp. 201-204 ◽  
Author(s):  
Jozef HANES ◽  
Johannes FREUDENSTEIN ◽  
Georg RAPP ◽  
Karl Heinz SCHEIT
Keyword(s):  
Elongation Factor ◽  
Coding Region ◽  
Elongation Factor 2 ◽  
Human Elongation Factor
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