Role of organic anion transporter 4 on the transport of olmesartan across the basal plasma membrane of human placental syncytiotrophoblast

Placenta ◽  
2016 ◽  
Vol 45 ◽  
pp. 133
Author(s):  
Masatoshi Tomi ◽  
Saki Noguchi ◽  
Ayasa Fujibayashi ◽  
Tetsuo Maruyama ◽  
Emi Nakashima ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Anion Transporter ◽  
Basal Plasma Membrane ◽  
Basal Plasma

scholarly journals Organic Anion Transporter 4-Mediated Transport of Olmesartan at Basal Plasma Membrane of Human Placental Barrier

2015 ◽  
Vol 104 (9) ◽  
pp. 3128-3135 ◽  
Author(s):  
Saki Noguchi ◽  
Tomohiro Nishimura ◽  
Ayasa Fujibayashi ◽  
Tetsuo Maruyama ◽  
Masatoshi Tomi ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Placental Barrier ◽  
Anion Transporter ◽  
Basal Plasma Membrane ◽  
Basal Plasma

scholarly journals Role of OAT4 in Uptake of Estriol Precursor 16α-Hydroxydehydroepiandrosterone Sulfate Into Human Placental Syncytiotrophoblasts From Fetus

Endocrinology ◽  
2015 ◽  
Vol 156 (7) ◽  
pp. 2704-2712 ◽  
Author(s):  
Masatoshi Tomi ◽  
Hiromi Eguchi ◽  
Mayuko Ozaki ◽  
Tomohiro Tawara ◽  
Sachika Nishimura ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Organic Anion ◽  
Dehydroepiandrosterone Sulfate ◽  
Cellular Level ◽  
Organic Anion Transporter ◽  
Organic Anion Transporting Polypeptide ◽  
Anion Transporter ◽  
Basal Plasma ◽  
Good Agreement

Estriol biosynthesis in human placenta requires the uptake of a fetal liver-derived estriol precursor, 16α-hydroxydehydroepiandrosterone sulfate (16α-OH DHEAS), by placental syncytiotrophoblasts at their basal plasma membrane (BM), which faces the fetal circulation. The aim of this work is to identify the transporter(s) mediating 16α-OH DHEAS uptake at the fetal side of syncytiotrophoblasts by using human placental BM-enriched vesicles and to examine the contribution of the putative transporter to estriol synthesis at the cellular level, using choriocarcinoma JEG-3 cells. Organic anion transporter (OAT)-4 and organic anion transporting polypeptide 2B1 proteins were enriched in human placental BM vesicles compared with crude membrane fraction. Uptake of [3H]16α-OH DHEAS by BM vesicles was partially inhibited in the absence of sodium but was significantly increased in the absence of chloride and after preloading glutarate. Uptake of [3H]16α-OH DHEAS by BM vesicles was significantly inhibited by OAT4 substrates such as dehydroepiandrosterone sulfate, estrone-3-sulfate, and bromosulfophthalein but not by cyclosporin A, tetraethylammonium, p-aminohippuric acid, or cimetidine. These characteristics of vesicular [3H]16α-OH DHEAS uptake are in good agreement with those of human OAT4-transfected COS-7 cells as well as forskolin-differentiated JEG-3 cells. Estriol secretion from differentiated JEG-3 cells was detected when the cells were incubated with 16α-OH DHEAS for 8 hours but was inhibited in the presence of 50 μM bromosulfophthalein. Our results indicate that OAT4 at the BM of human placental syncytiotrophoblasts plays a predominant role in the uptake of 16α-OH DHEAS for placental estriol synthesis.

scholarly journals SLCO4A1-AS1 Facilitates the Malignant Phenotype via miR-149-5p/STAT3 Axis in Gastric Cancer Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Qing Li ◽  
Dachuan Zhang ◽  
Hui Wang ◽  
Jun Xie ◽  
Lei Peng ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Gastric Cancer Cells ◽  
Anion Transporter ◽  
Transporter Family ◽  
Cancer Tissues ◽  
Development Of Gastric Cancer

Solute carrier organic anion transporter family member 4A1 (SLCO4A1-AS1), a newly discovered lncRNA, may exert effects in tumors. Since its role in gastric cancer remains obscure, we sought to explore the mechanism of SLCO4A1-AS1 in gastric cancer. The relationship among SLCO4A1-AS1, miR-149-5p, and STAT3 was detected by bioinformatics, dual luciferase analysis, and Pearson’s test, and the expressions of these genes were determined by quantitative real-time PCR and Western blot. Moreover, CCK-8, flow cytometry, wound healing assay, and Transwell analysis were performed to verify the function of SLCO4A1-AS1 in gastric cancer. Rescue experiments were used to detect the role of miR-149-5p. The expressions of SLCO4A1-AS1 and STAT3 were increased, while the expression of miR-149-5p was suppressed in gastric cancer tissues and cell lines. In addition, STAT3 expression was negatively correlated with miR-149-5p expression but was positively correlated with SLCO4A1-AS1 expression. Overexpression of SLCO4A1-AS1 promoted cell viability, migration, invasion, and STAT3 expression but suppressed apoptosis, while knockdown of SLCO4A1-AS1 had the opposite effect. SLCO4A1-AS1 bound to miR-149-5p and targeted STAT3. Moreover, miR-149-5p mimic inhibited the malignant development of gastric cancer cells and obviously reversed the function of SLCO4A1-AS1 overexpression. Our research reveals that abnormally increased SLCO4A1-AS1 expression may be an important molecular mechanism in the development of gastric cancer.

scholarly journals Role of human organic anion transporter 4 in the transport of ochratoxin A

2002 ◽  
Vol 1590 (1-3) ◽  
pp. 64-75 ◽  
Author(s):  
Ellappan Babu ◽  
Michio Takeda ◽  
Shinichi Narikawa ◽  
Yukari Kobayashi ◽  
Atsushi Enomoto ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Anion Transporter

Role of organic anion transporter 3 (Oat3) in the renal transport of glutathione (GSH)

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Lawrence H. Lash ◽  
David A. Putt ◽  
Feng Xu ◽  
Larry H. Matherly
Keyword(s):  
Organic Anion ◽  
Organic Anion Transporter ◽  
Renal Transport ◽  
Anion Transporter ◽  
Organic Anion Transporter 3
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