Short-term light adaptation of a cyanobacterium, Synechocystis sp. PCC 6803, probed by time-resolved fluorescence spectroscopy

2014 ◽  
Vol 81 ◽  
pp. 149-154 ◽  
Author(s):  
Seiji Akimoto ◽  
Makio Yokono ◽  
Erina Yokono ◽  
Shimpei Aikawa ◽  
Akihiko Kondo
Keyword(s):  
Fluorescence Spectroscopy ◽  
Light Adaptation ◽  
Time Resolved Fluorescence ◽  
Short Term ◽  
Time Resolved ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria Thermosynechococcus vulcanus NIES 2134 and Synechocystis sp. PCC 6803

Crystals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1233
Author(s):  
Mingyuan Xie ◽  
Wenjun Li ◽  
Chao Xiao ◽  
Zhanghe Zhen ◽  
Jianfei Ma ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Spectroscopy ◽  
Dynamic Process ◽  
High Efficiency ◽  
Excitation Energy Transfer ◽  
High Rate ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Synechocystis Sp ◽  
Pcc 6803

As the largest light-harvesting complex in cyanobacteria, phycobilisomes (PBSs) show high efficiency and a high rate of energy transfer, owing to an elegant antenna-like assembly. To understand the structural influence on the dynamic process of the energy transfer in PBSs, two cyanobacterium species Thermosynechococcus vulcanus NIES 2134 (T. 2134) and Synechocystis sp. PCC 6803 (S. 6803) with different rod–core-linked assemblies were chosen for this study. The dynamic process of the energy transfer in both PBSs was investigated through time-resolved fluorescence spectroscopy (TRFS) with a time resolution of sub-picosecond. Via the fluorescence decay curves deconvolution, the pathways and related rates of the excitation energy transfer (EET) were determined. Three time components, i.e., 10, 80, and 1250 ps, were identified in the EET in the PBSs of T. 2134 and three, i.e., 9, 115, and 1680 ps, in the EET in the PBSs of S. 6803. In addition, a comparison of the dynamic process of the energy transfer between the two cyanobacteria revealed how the PBS assembly affects the energy transfer in PBSs. The findings will provide insight into future time-resolved crystallography.

Time-resolved analysis of short term metabolic adaptation at dark transition in Synechocystis sp. PCC 6803

2019 ◽  
Vol 128 (4) ◽  
pp. 424-428 ◽  
Author(s):  
Masaharu Maruyama ◽  
Hiroki Nishiguchi ◽  
Masakazu Toyoshima ◽  
Nobuyuki Okahashi ◽  
Fumio Matsuda ◽  
...  
Keyword(s):  
Metabolic Adaptation ◽  
Short Term ◽  
Time Resolved ◽  
Synechocystis Sp ◽  
Dark Transition ◽  
Pcc 6803

scholarly journals Drug/protein interactions studied by time-resolved fluorescence spectroscopy

2014 ◽  
Author(s):  
Thomas Gustavsson ◽  
Dimitra Markovitsi ◽  
Ignacio Vayá ◽  
Paula Bonancía ◽  
M. C. Jiménez ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Protein Interactions ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Mechanism of charge transfer in the molecular DPQ complex studied by time-resolved fluorescence spectroscopy

1991 ◽  
Vol 95 (17) ◽  
pp. 6437-6440 ◽  
Author(s):  
V. V. Borovkov ◽  
R. P. Evstigneeva ◽  
I. A. Struganova ◽  
V. F. Kamalov ◽  
B. N. Toleutaev
Keyword(s):  
Charge Transfer ◽  
Fluorescence Spectroscopy ◽  
Time Resolved Fluorescence ◽  
Time Resolved
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