Construction of chromosome segment substitution lines of Dongxiang common wild rice (Oryza rufipogon Griff.) in the background of the japonica rice cultivar Nipponbare (Oryza sativa L.)

2019 ◽  
Vol 144 ◽  
pp. 274-282 ◽  
Author(s):  
Xiaoding Ma ◽  
Bing Han ◽  
Jianghong Tang ◽  
Jiani Zhang ◽  
Di Cui ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Wild Rice ◽  
Oryza Sativa L ◽  
Rice Cultivar ◽  
Oryza Rufipogon ◽  
Japonica Rice ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Oryza Rufipogon Griff ◽  
Segment Substitution

scholarly journals Development of Chromosome Segment Substitution Lines (CSSLs) Derived from Guangxi Wild Rice (Oryza rufipogon Griff.) under Rice (Oryza sativa L.) Background and the Identification of QTLs for Plant Architecture, Agronomic Traits and Cold Tolerance

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 980
Author(s):  
Ruizhi Yuan ◽  
Neng Zhao ◽  
Babar Usman ◽  
Liang Luo ◽  
Shanyue Liao ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Fine Mapping ◽  
Wild Rice ◽  
Oryza Rufipogon ◽  
Chromosome Segment ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Genome Wide ◽  
Oryza Rufipogon Griff ◽  
Segment Substitution

Common wild rice contains valuable resources of novel alleles for rice improvement. It is well known that genetic populations provide the basis for a wide range of genetic and genomic studies. In particular, chromosome segment substitution lines (CSSLs) ais a powerful tool for fine mapping of quantitative traits, new gene discovery and marker-assisted breeding. In this study, 132 CSSLs were developed from a cultivated rice (Oryza sativa) cultivar (93-11) and common wild rice (Oryza rufipogon Griff. DP30) by selfing-crossing, backcrossing and marker-assisted selection (MAS). Based on the high-throughput sequencing of the 93-11 and DP30, 285 pairs of Insertion-deletions (InDel) markers were selected with an average distance of 1.23 Mb. The length of this DP30-CSSLs library was 536.4 cM. The coverage rate of substitution lines cumulatively overlapping the whole genome of DP30 was about 91.55%. DP30-CSSLs were used to analyze the variation for 17 traits leading to the detection of 36 quantitative trait loci (QTLs) with significant phenotypic effects. A cold-tolerant line (RZ) was selected to construct a secondary mapping F2 population, which revealed that qCT2.1 is in the 1.7 Mb region of chromosome 2. These CSSLs may, therefore, provide powerful tools for genome wide large-scale gene discovery in wild rice. This research will also facilitate fine mapping and cloning of QTLs and genome-wide study of wild rice. Moreover, these CSSLs will provide a foundation for rice variety improvement.

Salt-responsive mechanisms in chromosome segment substitution lines of rice (Oryza sativa L. cv. KDML105)

2016 ◽  
Vol 103 ◽  
pp. 96-105 ◽  
Author(s):  
Noppawan Nounjan ◽  
Jonaliza L. Siangliw ◽  
Theerayut Toojinda ◽  
Supachitra Chadchawan ◽  
Piyada Theerakulpisut
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Chromosome Segment ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Segment Substitution
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