Biological control activity of Trichoderma asperelloides PSU-P1 against gummy stem blight in muskmelon (Cucumis melo)

Deteksi Gen Ketahanan Terhadap Gsb-4 (Gummy Stem Blight) pada Tanaman Melon (Cucumis Melo L.)

Journal of Biota ◽

10.24002/biota.v1i2.993 ◽

2017 ◽

Vol 1 (2) ◽

Author(s):

Ganies Riza Aristya Aisha Rizky Rahmawati Budi Setiadi Daryono

Keyword(s):

Simple Sequence Repeat ◽

Cucumis Melo ◽

Sequence Repeat ◽

Stem Blight ◽

Gummy Stem Blight ◽

Cucumis Melo L ◽

Simple Sequence

Perakitan melon tahan penyakit gummy stem blight (Gsb-4) merupakan upaya yang dapat dilakukan untuk menanggulangi penyakit gummy stem blight. Untuk itu harus diketahui ada tidaknya gen ketahanan terhadap gummy stem blight. Tujuan penelitian ini adalah mendeteksi adanya gen ketahanan terhadap gummy stem blight pada 18 kultivar melon dan 1 kultivar mentimun. Hasil amplifikasi menggunakan penanda molekular Simple Sequence Repeat (SSR) dengan primer spesifik yaitu CMTA170a menunjukkan pita berukuran 120 bp sedangkan dengan primer CMCT160a+b menunjukkan pita berukuran 212 bp. Hasil yang diuji menunjukkan bahwa semua kultivar melon memiliki gen ketahanan terhadap gummy stem blight baik homozigot maupun heterozigot.

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Resistance to Gummy Stem Blight in Muskmelon

HortScience ◽

10.21273/hortsci.28.9.930 ◽

1993 ◽

Vol 28 (9) ◽

pp. 930-931 ◽

Cited By ~ 13

Author(s):

D.J. McGrath ◽

L. Vawdrey ◽

I.O. Walker

Keyword(s):

Cucumis Melo ◽

Didymella Bryoniae ◽

Disease Reaction ◽

Stem Blight ◽

Gummy Stem Blight ◽

Alternative Sources

Resistance to gummy stem blight [Didymella bryoniae (Auersw.) Rehm] was evaluated in two accessions of Cucumis melo L., PI 266935 and PI 266934. Based on disease reaction scores and dry weights, PI 266934 possessed much greater resistance than PI 266935. The quality of resistance of PI 266934 was unaffected by the ranges of seedling ages and inoculum concentrations used. No melon cultivar, to our knowledge, is highly resistant to gummy stem blight in the field, and alternative sources of superior resistance are potentially useful for breeding.

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Screening Melon (Cucumis melo) for Resistance to Gummy Stem Blight in the Greenhouse and Field

HortScience ◽

10.21273/hortsci.32.1.117 ◽

1997 ◽

Vol 32 (1) ◽

pp. 117-121 ◽

Cited By ~ 14

Author(s):

Yiping Zhang ◽

Molly Kyle ◽

Konstantinos Anagnostou ◽

Thomas A. Zitter

Keyword(s):

Cucumis Melo ◽

Field Tests ◽

Plant Introduction ◽

Didymella Bryoniae ◽

Stem Blight ◽

Gummy Stem Blight ◽

High Level ◽

Different Origins ◽

Chinese Group ◽

Onondaga County

Greenhouse and field evaluations of melon (Cucumis melo L.) for resistance to gummy stem blight, caused by the fungus Didymella bryoniae (Auersw.) Rehm, were conducted on 798 U.S. Dept. of Agriculture Plant Introduction (PI) accessions and 24 related Cucumis species. Plants were inoculated at the three to four true-leaf stage with a virulent isolate of D. bryoniae collected from Onondaga County, N.Y., and disease indices were calculated based on foliar and stem symptoms. In greenhouse screens, 43 C. melo accessions showed a high level of resistance. Results were consistent between the optimized greenhouse screening procedure described and inoculated replicated field tests. Of these accessions, a Chinese group, PIs 157076, 157080, 157081, 157082, 157084; another group from Zimbabwe, PIs 482393, 482398, 482399, 482402, 482403, 482408; and some others from different origins, PI 255478 (Korea) and PI 511890 (Mexico), showed high levels of resistance, at least equal to that in PI 140471, the leading source of resistance to date.

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Evaluation of fungicides and cultivars for control of gummy stem blight of rockmelon caused by Didymella bryoniae

Australian Journal of Experimental Agriculture ◽

10.1071/ea9941191 ◽

1994 ◽

Vol 34 (8) ◽

pp. 1191 ◽

Cited By ~ 7

Author(s):

LL Vawdrey

Keyword(s):

Cucumis Melo ◽

The United States ◽

United States Department ◽

Didymella Bryoniae ◽

Department Of Agriculture ◽

States Department ◽

Stem Blight ◽

Gummy Stem Blight ◽

Commercial Cultivars ◽

Marketable Fruit

Ten fungicides were evaluated in 2 experiments with rockmelons for control of gummy stem blight caused by Didymella bryoniae. The fungicides (g a.i./ha) tebuconazole (100 and 160), fentin hydroxide (226), prochloraz Mn (231 and 370), benomyl (400), benomyl (400, 500) plus white oil (2 L/ha), propiconazole (100), mancozeb (1600), mancozeb plus phosphonic acid (1600 + 1000), myclobutanil (237), and chlorothalonil (1600) significantly (P<0.05) reduced the incidence and severity of gummy stem blight compared with an unsprayed treatment. Total weight of fruit and number and weight of marketable fruit in plots treated with fungicides were increased (P<0.05) by as much as 55% compared with untreated plots. Premature fruit ripening was reduced where there was effective disease control. Tebuconazole was the superior chemical but was phytotoxic when applied at 160 g a.i./ha. Fentin hydroxide caused burn on old foliage. Two accessions of Cucumis melo obtained from the United States Department of Agriculture (PI 266934, PI 266935) and 5 commercial cultivars were evaluated for resistance to gummy stem blight in a field experiment. PI 266934 and PI 266935 were highly resistant and the commercial cultivars were susceptible.

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First Report of Pycnidial Stage of Didymella bryoniae, the Causal Fungus of Gummy Stem Blight on Cantaloupe (Cucumis melo var cantalupensis) in Egypt

Egyptian Journal of Phytopathology ◽

10.21608/ejp.2016.92386 ◽

2016 ◽

Vol 44 (1) ◽

pp. 231-232

Author(s):

Abdel-Fattah El-Wakil ◽

Amal Khalil

Keyword(s):

Cucumis Melo ◽

Didymella Bryoniae ◽

First Report ◽

Stem Blight ◽

Gummy Stem Blight ◽

Causal Fungus

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Resistance to Gummy Stem Blight in Melon (Cucumis melo L.) Germplasm and Inheritance of Resistance from Plant Introductions 157076, 420145, and 323498

HortScience ◽

10.21273/hortsci.42.2.215 ◽

2007 ◽

Vol 42 (2) ◽

pp. 215-221 ◽

Cited By ~ 13

Author(s):

Joseph N. Wolukau ◽

Xiao-Hui Zhou ◽

Ying Li ◽

Yong-Bin Zhang ◽

Jin-Feng Chen

Keyword(s):

Cucumis Melo ◽

Dominant Gene ◽

Plant Introduction ◽

Didymella Bryoniae ◽

Genetics Of Resistance ◽

Sources Of Resistance ◽

Resistant Parent ◽

Plant Introductions ◽

Stem Blight ◽

Gummy Stem Blight

Gummy stem blight incited by the fungus Didymella bryoniae is a major disease of melons worldwide. The objectives of the present study were to critically evaluate melon (Cucumis melo L.) germplasm for resistance to D. bryoniae and to characterize the genetics of resistance in the resistant accessions. Two hundred sources of germplasm (plant introduction accessions, cultivars, breeding lines, landraces, and wild relatives) were screened against a single highly virulent isolate (IS25) of D. bryoniae in a plastic tunnel. The genetics of resistance to D. bryoniae was studied in three crosses between plant introductions 157076, 420145, and 323498, resistant parents that were fairly adapted (flowering, fruiting, powdery mildew tolerance) to Nanjing conditions, and plant introductions 268227, 136170, and NSL 30032 susceptible parents, respectively. Six populations of each cross (susceptible parent, resistant parent, F1, F2, the two reciprocal backcrosses) were analyzed for their responses to D. bryoniae. Seedlings in both studies were inoculated with a spore suspension (5 × 105 spores/mL−1) of D. bryoniae at the four to six true-leaf stages and assessed for leaf and stem damage at 7, 14, and 21 d postinoculation. Results of germplasm screening indicated most germplasms reported as resistant elsewhere were confirmed resistant under our conditions. However, some plant introductions identified as highly resistant elsewhere were susceptible under our conditions, the most interesting being plant introduction 482399. This plant introduction that was considered resistant was highly susceptible in our study. We also identified other sources of resistance not reported previously, for example, JF1; a wild Cucumis from the highlands of Kenya was rated highly resistant. Analysis of segregation of F1, F2, and backcross generations of the three crosses indicated that each of the three plant introductions carry a single dominant gene for resistance to the D. bryoniae.

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Five independent loci each control monogenic resistance to gummy stem blight in melon (Cucumis melo L.)

Theoretical and Applied Genetics ◽

10.1007/s00122-003-1519-2 ◽

2003 ◽

Vol 108 (6) ◽

pp. 1033-1038 ◽

Cited By ~ 16

Author(s):

J. D. Frantz ◽

M. M. Jahn

Keyword(s):

Cucumis Melo ◽

Stem Blight ◽

Gummy Stem Blight ◽

Monogenic Resistance ◽

Cucumis Melo L

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Differential Susceptibility of Nine Cucurbit Species to the Foliar Blight and Crown Canker Phases of Gummy Stem Blight

Plant Disease ◽

10.1094/pdis-05-13-0510-re ◽

2014 ◽

Vol 98 (2) ◽

pp. 247-254 ◽

Cited By ~ 15

Author(s):

Anthony P. Keinath

Keyword(s):

Cucumis Melo ◽

Citrullus Lanatus ◽

Differential Susceptibility ◽

Cucurbita Moschata ◽

Didymella Bryoniae ◽

Stem Blight ◽

Gummy Stem Blight ◽

Foliar Blight ◽

Diseased Leaf Area ◽

Stem Cankers

Eighteen cucurbit cultivars representing five genera, nine species, and 17 horticultural types were inoculated with Didymella bryoniae in field plots in Charleston, SC, in autumn 2008, autumn 2009, and spring 2011 to determine susceptibility to gummy stem blight. In each year, gummy stem blight cankers occurred more frequently on crowns or main stems of ‘Athena’ muskmelon (Cucumis melo) and ‘Green Striped Cushaw’ squash (Cucurbita argyrosperma) than on all other cultivars except ‘Rocio’ honeydew (Cucumis melo) in 2009, and bottle gourd (Lagenaria siceraria) and Ojakkyo citron (Citrullus lanatus var. citroides) in 2011. Cucurbita moschata was highly resistant to stem cankers. Severity of gummy stem blight on foliage was moderate to severe in autumn 2009, mild to severe in autumn 2008, and very mild in spring 2011 (due to unusually dry weather). Watermelon (Citrullus lanatus var. lanatus) and melon were the most susceptible to foliar blight in 2008 and 2009. In all 3 years, ‘Cheese’ and ‘Bugle’ squash (both Cucurbita moschata), and ‘Judgment III’ and ‘Lioness’ summer squash (C. pepo) were among the cultivars with the least diseased leaf area. C. moschata, C. pepo, C. maxima, and Luffa cylindrica (smooth loofah) were significantly less susceptible to foliar blight than Cucumis melo and three Citrullus lanatus cultivars in 2008 and 2009. This study also is the first documentation of susceptibility of Cucurbita argyrosperma to gummy stem blight.

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