scholarly journals Deteksi Gen Ketahanan Terhadap Gsb-4 (Gummy Stem Blight) pada Tanaman Melon (Cucumis Melo L.)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Ganies Riza Aristya Aisha Rizky Rahmawati Budi Setiadi Daryono
Keyword(s):  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Sequence Repeat ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Cucumis Melo L ◽  
Simple Sequence

Perakitan melon tahan penyakit gummy stem blight (Gsb-4) merupakan upaya yang dapat dilakukan untuk menanggulangi penyakit gummy stem blight. Untuk itu harus diketahui ada tidaknya gen ketahanan terhadap gummy stem blight. Tujuan penelitian ini adalah mendeteksi adanya gen ketahanan terhadap gummy stem blight pada 18 kultivar melon dan 1 kultivar mentimun. Hasil amplifikasi menggunakan penanda molekular Simple Sequence Repeat (SSR) dengan primer spesifik yaitu CMTA170a menunjukkan pita berukuran 120 bp sedangkan dengan primer CMCT160a+b menunjukkan pita berukuran 212 bp. Hasil yang diuji menunjukkan bahwa semua kultivar melon memiliki gen ketahanan terhadap gummy stem blight baik homozigot maupun heterozigot.

Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.)

2005 ◽  
Vol 110 (5) ◽  
pp. 802-811 ◽  
Author(s):  
M. J. Gonzalo ◽  
M. Oliver ◽  
J. Garcia-Mas ◽  
A. Monfort ◽  
R. Dolcet-Sanjuan ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Linkage Maps ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Cucumis Melo L ◽  
Simple Sequence

scholarly journals Genetic Diversity and Population Structure of Traditional Greek and Cypriot Melon Cultigens (Cucumis melo L.) Based on Simple Sequence Repeat Variability

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 1820-1824 ◽  
Author(s):  
Emmanouil N. Tzitzikas ◽  
Antonio J. Monforte ◽  
Abdelhak Fatihi ◽  
Zacharias Kypriotakis ◽  
Tefkros A. Iacovides ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variability ◽  
Ssr Markers ◽  
Structure Analysis ◽  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Sequence Repeat ◽  
Population Structure Analysis ◽  
Simple Sequence

Seventeen simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure among traditional Greek and Cypriot melon cultigens (Cucumis melo L.). All SSR markers were polymorphic with a total number of 81 alleles, whereas all cultigens could be distinguished with at least one SSR, except cultigens 43 and 41. Reference accessions showed larger genetic variability with an average of four alleles per locus and 0.65 gene of diversity compared with an average of 2.47 alleles per locus and 0.30 of gene diversity for the Greek/Cypriot cultigens. Observed heterozygosity was very low, indicating a lack of outcrossing, at least in recent times. Unrooted neighbor-joining tree analysis and population structure analysis clustered the cultigens and the reference genotypes into five groups. All cultigens could be distinguished; the Cypriot cultigens were more closely related to the inodorus ‘Piel de Sapo’, whereas the Greek cultigens were located in an intermediate position between the inodorus ‘Piel de Sapo’ and the cantalupensis ‘Védrantais’. The cultigen ‘Kokkini’ was the most divergent among the Greek and Cypriot cultigens. This association between geographic origin and genetic similarity among Greek and Cypriot cultigens indicates geographic isolation. Most of the cultivars from the same cultivar group (i.e., inodorus, cantalupensis) clustered together, but some exceptions were found, suggesting that former inodorus landraces would have been transformed to cantalupensis as a result of intercrossing and further selection by farmers. Results of population structure analysis support mixing between cantalupensis and inodorus. ‘Agiou Basileiou’, an inodorus cultigen, was assigned to the subpopulation IV/II of which II is a pure cantalupensis subpopulation. Greek and Cypriot melon cultigens were developed from a broader germplasm base than western Mediterranean cultivars and exhibited useful for melon breeding programs genetic variability.

scholarly journals The Determination of the Genetic Distance of Various Snake Melon Cucumis melo var. flexuosus Cultivars Using Inter Simple Sequence Repeat Technique (ISSR)

2021 ◽  
Vol 34 (1) ◽  
pp. 111-123
Author(s):  
Haidar A. K. Al Khazraji ◽  
Abdulkareem M. Abd ◽  
Abdulla A. Abdulla
Keyword(s):  
Cluster Analysis ◽  
Genetic Distance ◽  
Ethidium Bromide ◽  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Inter Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence

The experiment have been done on the winter farming season 2020 in one of the farms that belongs to Faris company in Basrah governorate, the technique ISSR have been used to study the genetic distance for twenty one isolates of snake melon Cucumis melo var. flexuosus species. The variations between amplified samples have been revealed after running them on a gel of agarose which have been previously stained by ethidium bromide. Five primers which gave varied product on the agarose have been selected. Those five primers produced 713 bands, both primers UBC 813 and UBC 815 showed the higher numbers of bands reached to 177 while the primer UBC862 showed the least numbers of bands (100) and the bands which showed multi-variations showed (46) bands, and the results primers amplification unique bands their number reached to (14) and five of those bands belong to the primer UBC842 while the primer UBC862 produced three bands while the primer UBC807 did not produced any bands. While it has shown 100% polymorphisms with the primers 813, 815, 842 and 862, and the least polymorphism percentage have shown with the primer UBC807 reached 75%. According to the efficiency primers, the highest efficiency percentage shown with the primer UBC813 and 815 reached to 24.82% and the least percentage shown 14.02% by the primer UBC862. Cluster analysis showed the effect on the variance of the studied cultivars.

scholarly journals Monogenic Dominant Resistance to Gummy Stem Blight in Two Melon (Cucumis melo) Accessions

Plant Disease ◽  
1999 ◽  
Vol 83 (12) ◽  
pp. 1105-1107 ◽  
Author(s):  
T. L. Zuniga ◽  
J. P. Jantz ◽  
T. A. Zitter ◽  
M. K. Jahn
Keyword(s):  
Cucumis Melo ◽  
Dominant Gene ◽  
Plant Introduction ◽  
Didymella Bryoniae ◽  
Susceptible Parent ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Backcross Populations ◽  
Relationship Of ◽  
Cucumis Melo L

Two melon (Cucumis melo L.) accessions, plant introduction (PI) 157082 and PI 511890, reported to be resistant to gummy stem blight, a disease incited by the fungus Didymella bryoniae, were crossed with a susceptible parent to determine the inheritance of resistance. Resistance in both accessions is due to a single dominant gene, based on analysis of F1, F2, and backcross populations. Additionally, PI 157082 was crossed with PI 140471, the other source of resistance identified to date, to examine the genetic relationship of resistance found in these two sources. The frequency of susceptible individuals from the (PI 157082 × 140471) F2 population was consistent with a 15:1 resistant:susceptible ratio, indicating that PIs 140471 and 157082 possess different resistance genes.

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