Multiple methods for varietal resistance assessment of durum wheat cultivars against Fusarium culmorum the causal agent of Fusarium Head Blight and Crown Rot in Algeria

Abstract Background Durum wheat (Triticum durum Desf.) is one of the most important cereals in the world. Unfortunately, the wheat plant is a target of several species of the genus Fusarium. This genus causes two serious diseases: fusarium crown rot (FCR) and fusarium head blight (FHB). The search for new indigenous strains of Trichoderma with a high potential for biocontrol against these two diseases was the purpose of this study. Results Biocontrol potential of 15 isolates of Trichoderma (T1 to T15), isolated from different rhizosphere soils and Algerian ecosystems, was evaluated against 4 strains of Fusarium culmorum (FC11, FC2, FC4, and FC20); the main causative agent of FCR and FHB. The efficacy of biological control by Trichoderma spp., evaluated by in vitro tests (direct and indirect confrontation), was confirmed by in vivo bioassays. The in vitro results showed a significant inhibition of mycelial growth of F. culmorum species than the control. The highest percentages of inhibition were obtained by T9, T12, and T14 isolates causing a maximum inhibition percentage of 81.81, 77.27, and 80.68%, respectively. T14 was selected for biocontrol in in vivo testing. A tube and pot experiments for FCR against F. culmorum showed that T14 decreased the disease severity with 50 and 63.63% reduction, respectively. FHB infection was significantly reduced by T14 in all durum wheat cultivars tested, where %AUDPC (area under the disease progress curve) reduction was 49.77, 43.43, 48.25, and 74.60% for Simeto, Waha, Bousselem, and Setifis genotypes, respectively. Yields also increased significantly for almost all cultivars. The antagonistic T14 was characterized based on molecular tools, using translation elongation factor1-alpha (TEF1-α) and internal transcribed spacers rDNA (ITS1). The results identified T14 as T. afroharzianum with accession numbers attributed by NCBI GenBank as MW171248 and MW159753. Conclusions Trichoderma afroharzianum, evaluated for the first time in Algeria as biocontrol agent, is a promising biocontrol approach against FCR and FHB.

Download Full-text

FcRav2 , a gene with a ROGDI domain involved in Fusarium head blight and crown rot on durum wheat caused by Fusarium culmorum

Molecular Plant Pathology ◽

10.1111/mpp.12551 ◽

2017 ◽

Vol 19 (3) ◽

pp. 677-688 ◽

Cited By ~ 3

Author(s):

Francesca Spanu ◽

Barbara Scherm ◽

Irene Camboni ◽

Virgilio Balmas ◽

Giovanna Pani ◽

...

Keyword(s):

Durum Wheat ◽

Fusarium Head Blight ◽

Fusarium Culmorum ◽

Crown Rot ◽

Head Blight

Download Full-text

Co-occurrence of Fusarium head blight and crown rot on several wheat cultivars in the South of Iraq Basra Province

10.1063/5.0000108 ◽

2020 ◽

Author(s):

Mohammed Hussein Minati ◽

Mohanned Khalaf Mohammed-Ameen

Keyword(s):

Fusarium Head Blight ◽

Crown Rot ◽

Wheat Cultivars ◽

The South ◽

Head Blight ◽

South Of Iraq

Download Full-text

Inheritances of Fusarium Head Blight Resistance in a Cross Involving Local and Exotic Durum Wheat Cultivars

Crop Science ◽

10.2135/cropsci2011.03.0140 ◽

2011 ◽

Vol 51 (6) ◽

pp. 2517-2524 ◽

Cited By ~ 14

Author(s):

M. M. Fakhfakh ◽

A. Yahyaoui ◽

S. Rezgui ◽

E. M. Elias ◽

A. Daaloul

Keyword(s):

Durum Wheat ◽

Fusarium Head Blight ◽

Fusarium Head Blight Resistance ◽

Blight Resistance ◽

Wheat Cultivars ◽

Head Blight

Download Full-text

Deoxynivalenol Detoxification in Transgenic Wheat Confers Resistance to Fusarium Head Blight and Crown Rot Diseases

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-06-18-0155-r ◽

2019 ◽

Vol 32 (5) ◽

pp. 583-592 ◽

Cited By ~ 12

Author(s):

Giulia Mandalà ◽

Silvio Tundo ◽

Sara Francesconi ◽

Federica Gevi ◽

Lello Zolla ◽

...

Keyword(s):

Transgenic Plants ◽

Durum Wheat ◽

Fusarium Head Blight ◽

Bread Wheat ◽

Virulence Factor ◽

Fusarium Species ◽

Crown Rot ◽

Uridine Diphosphate ◽

Head Blight ◽

Principal Mechanism

Fusarium diseases, including Fusarium head blight (FHB) and Fusarium crown rot (FCR), reduce crop yield and grain quality and are major agricultural problems worldwide. These diseases also affect food safety through fungal production of hazardous mycotoxins. Among these, deoxynivalenol (DON) acts as a virulence factor during pathogenesis on wheat. The principal mechanism underlying plant tolerance to DON is glycosylation by specific uridine diphosphate–dependent glucosyltransferases (UGTs), through which DON-3-β-d-glucoside (D3G) is produced. In this work, we tested whether DON detoxification by UGT could confer to wheat a broad-spectrum resistance against Fusarium graminearum and F. culmorum. These widespread Fusarium species affect different plant organs and developmental stages in the course of FHB and FCR. To assess DON-detoxification potential, we produced transgenic durum wheat plants constitutively expressing the barley HvUGT13248 and bread wheat plants expressing the same transgene in flower tissues. When challenged with F. graminearum, FHB symptoms were reduced in both types of transgenic plants, particularly during early to mid-infection stages of the infection progress. The transgenic durum wheat displayed much greater DON-to-D3G conversion ability and a considerable decrease of total DON+D3G content in flour extracts. The transgenic bread wheat exhibited a UGT dose–dependent efficacy of DON detoxification. In addition, we showed, for the first time, that DON detoxification limits FCR caused by F. culmorum. FCR symptoms were reduced throughout the experiment by nearly 50% in seedlings of transgenic plants constitutively expressing HvUGT13248. Our results demonstrate that limiting the effect of the virulence factor DON via in planta glycosylation restrains FHB and FCR development. Therefore, ability for DON detoxification can be a trait of interest for wheat breeding targeting FHB and FCR resistance.

Download Full-text

Fusarium Head Blight of Common Polish Winter Wheat Cultivars - Comparison of Effects of Fusarium avenaceum and Fusarium culmorum on Yield Components

Journal of Phytopathology ◽

10.1046/j.1439-0434.2002.00723.x ◽

2002 ◽

Vol 150 (3) ◽

pp. 135-141 ◽

Cited By ~ 19

Author(s):

P. GOLINSKI ◽

Z. KACZMAREK ◽

I. KIECANA ◽

H. WISNIEWSKA ◽

P. KAPTUR ◽

...

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Yield Components ◽

Fusarium Culmorum ◽

Fusarium Avenaceum ◽

Wheat Cultivars ◽

Head Blight ◽

Winter Wheat Cultivars

Download Full-text

A valuable QTL for Fusarium head blight resistance from Triticum turgidum L. ssp. dicoccoides has a stable expression in durum wheat cultivars

Cereal Research Communications ◽

10.1556/0806.45.2017.007 ◽

2017 ◽

Vol 45 (2) ◽

pp. 234-247 ◽

Cited By ~ 1

Author(s):

D. Soresi ◽

D. Zappacosta ◽

A. Garayalde ◽

I. Irigoyen ◽

J. Basualdo ◽

...

Keyword(s):

Durum Wheat ◽

Fusarium Head Blight ◽

Triticum Turgidum ◽

Fusarium Head Blight Resistance ◽

Stable Expression ◽

Blight Resistance ◽

Wheat Cultivars ◽

Head Blight

Download Full-text

Progression of Deoxynivalenol Concentrations in Spikes and Kernels of Winter Wheat Cultivars after Inoculation with Fusarium culmorum

Czech Journal of Genetics and Plant Breeding ◽

10.17221/3644-cjgpb ◽

2011 ◽

Vol 42 (No. 4) ◽

pp. 137-141 ◽

Cited By ~ 1

Author(s):

J. Chrpová ◽

V. Šíp ◽

E. Matějová ◽

S. Sýkorová

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Crucial Role ◽

Early Stage ◽

Fusarium Culmorum ◽

Wheat Cultivars ◽

Head Blight ◽

Winter Wheat Cultivars ◽

High Concentrations

Progression of deoxynivalenol (DON) concentrations in spikes and kernels was studied in relation to Fusarium head blight (FHB) symptoms in five winter wheat cultivars, differing in resistance to FHB, after single floret inoculation with an aggressive isolate of Fusarium culmorum. After inoculation in field conditions the spikes were detached from the plant and kept in the greenhouse under controlled conditions. High concentrations of DON were detected in susceptible cultivars at an early stage of pathogenesis (7 days after inoculation). Over the whole examined 21-day period and also at maturity spikes contained more DON than kernels. While differences between cultivars in the accumulation of DON were highly expressed already 7 days after inoculation, differences in symptomatic reactions were not clear until day 21. Owing to the reported crucial role of DON at early stages of pathogenesis, the importance of appropriate timing of fungicide application is highly stressed.  

Download Full-text