The association between Mycobacterium avium subsp. paratuberculosis fecal shedding or clinical Johne's disease and lactation performance on two Minnesota, USA dairy farms

ABSTRACT Mycobacterium avium subsp. paratuberculosis, the causative agent of Johne's disease in cattle, was identified in settled-dust samples of Dutch commercial dairy farms, both in the dairy barn and in the young stock housing. Bioaerosols may play a role in within-farm M. avium subsp. paratuberculosis transmission.

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Use of enoyl coenzyme A hydratase of Mycobacterium avium subsp. paratuberculosis for the serological diagnosis of Johne's disease

Veterinary Immunology and Immunopathology ◽

10.1016/j.vetimm.2013.06.020 ◽

2013 ◽

Vol 155 (4) ◽

pp. 253-258 ◽

Cited By ~ 5

Author(s):

Reiko Nagata ◽

Satoko Kawaji ◽

Yasuyuki Mori

Keyword(s):

Mycobacterium Avium ◽

Coenzyme A ◽

Johne's Disease ◽

Johne’S Disease ◽

Serological Diagnosis ◽

Mycobacterium Avium Subsp ◽

Mycobacterium Avium Subsp Paratuberculosis

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Experimental animal infection models for Johne’s disease, an infectious enteropathy caused by Mycobacterium avium subsp. paratuberculosis

The Veterinary Journal ◽

10.1016/j.tvjl.2007.02.022 ◽

2008 ◽

Vol 176 (2) ◽

pp. 129-145 ◽

Cited By ~ 54

Author(s):

Douglas J. Begg ◽

Richard J. Whittington

Keyword(s):

Experimental Animal ◽

Mycobacterium Avium ◽

Johne's Disease ◽

Johne’S Disease ◽

Mycobacterium Avium Subsp ◽

Infection Models ◽

Animal Infection ◽

Mycobacterium Avium Subsp Paratuberculosis

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The collection of lymphatic fluid from the bovine udder and its use for the detection of Mycobacterium avium subsp. paratuberculosis in the cow

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638711425943 ◽

2011 ◽

Vol 24 (1) ◽

pp. 23-31 ◽

Cited By ~ 5

Author(s):

Johannes L. Khol ◽

Pablo J. Pinedo ◽

Claus D. Buergelt ◽

Laura M. Neumann ◽

Walter Baumgartner ◽

...

Keyword(s):

Mycobacterium Avium ◽

Fluid Collection ◽

Johne's Disease ◽

Johne’S Disease ◽

Mycobacterium Avium Subsp ◽

Enzyme Linked Immunosorbent Assay ◽

Lymph Fluid ◽

Mycobacterium Avium Subsp Paratuberculosis ◽

Lymphatic Fluid ◽

Elisa Result

The objective of the current study was to evaluate the feasibility of lymph collection from the bovine udder and to investigate if the lymphatic fluid might be of diagnostic value in cows infected with Mycobacterium avium subsp. paratuberculosis (MAP), the etiologic agent of paratuberculosis. Lymph fluid collection was attempted from 58 cows, and the reactions of the cows as well as the level of difficulty of the procedure were recorded in 56 animals. Lymph samples (51 in total) were tested for the presence of MAP by nested polymerase chain reaction. Collection of the lymphatic fluid caused no or mild signs of discomfort in 94.6% of the cows; in 51.8% of cows, lymphatic fluid was attained on the first attempt, while sample collection was unsuccessful in 12.1%. Mycobacterium avium subsp. paratuberculosis was detected in 43.1% of all lymph samples. The bacterium was present in 66.7% of cows with clinical Johne’s disease, in 42.8% of asymptomatic cows with a positive or suspicious enzyme-linked immunosorbent assay (ELISA) result in blood, and in 38.7% of cows with a negative ELISA result in blood. The present study shows that the procedure was well tolerated by most cows and can easily be performed on farm. The current report of the isolation of MAP from lymph fluid suggests that the present approach could be used for the early detection of Johne’s disease in cattle.

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