Rhizopus oryzae IFO 4697 whole cell catalyzed methanolysis of crude and acidified rapeseed oils for biodiesel production in tert-butanol system

Whole cell biocatalysts for biodiesel production have garnered significant attention in recent years, as they can help avoid the complex procedures of isolation, purification, and immobilization of extracellular lipase. Because of its renewability and biodegradability, loofah (Luffa cylindrica) sponge is an advantageous substitute for traditional biomass carriers in whole cell immobilization. Rhizopus oryzae mycelia can spontaneously attach onto loofah sponge particles (LSPs) during cell cultivation. The highest immobilized R. oryzae cells concentration can reach up to 1.40 g/1 g of LSPs. The effects of biocatalyst addition and water content on methanolysis for biodiesel production were investigated in this paper. The operational stability of glutaraldehyde-treated biocatalyst at 35 °C, using a 1:1 oil-to-methanol ratio, was assayed, revealing a 3.4-fold increase in half-life compared with the untreated biocatalyst. Under optimized conditions, the yield of methyl esters in the reaction mixture reached 82.2% to 92.2% in each cycle. These results suggested that loofah sponge is a potential fungi carrier for an immobilized whole-cell biocatalyst.

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Study on factors influencing stability of whole cell during biodiesel production in solvent-free and tert-butanol system

Biochemical Engineering Journal ◽

10.1016/j.bej.2008.04.003 ◽

2008 ◽

Vol 41 (2) ◽

pp. 111-115 ◽

Cited By ~ 33

Author(s):

Wei Li ◽

Wei Du ◽

Dehua Liu ◽

Yuan Yao

Keyword(s):

Solvent Free ◽

Biodiesel Production ◽

Whole Cell ◽

Tert Butanol ◽

Factors Influencing

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Improved catalytic performance of GA cross-linking treated Rhizopus oryzae IFO 4697 whole cell for biodiesel production

Process Biochemistry ◽

10.1016/j.procbio.2010.03.037 ◽

2010 ◽

Vol 45 (7) ◽

pp. 1192-1195 ◽

Cited By ~ 11

Author(s):

Ting Sun ◽

Wei Du ◽

Dehua Liu ◽

Lingmei Dai

Keyword(s):

Rhizopus Oryzae ◽

Catalytic Performance ◽

Biodiesel Production ◽

Cross Linking ◽

Whole Cell

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Codisplay of Rhizopus oryzae and Candida rugosa Lipases for Biodiesel Production

Catalysts ◽

10.3390/catal11040421 ◽

2021 ◽

Vol 11 (4) ◽

pp. 421

Author(s):

Xiaoxu Yang ◽

Yan Zhang ◽

Huimin Pang ◽

Sheng Yuan ◽

Xuxia Wang ◽

...

Keyword(s):

Rhizopus Oryzae ◽

Candida Rugosa ◽

Candida Rugosa Lipase ◽

Maximum Activity ◽

Biodiesel Production ◽

Rhizopus Oryzae Lipase ◽

Antibiotic Resistant ◽

Whole Cell ◽

Production Strategy ◽

Resistant Strains

In this study, we overcame the limitations of single-enzyme system catalysis by codisplaying Candida rugosa lipase 1 (CRL1) and Rhizopus oryzae lipase (ROL) on the cell surfaces of the whole-cell catalyst Pichia pastoris to produce biodiesel from tallow seed oil. We screened double antibiotic-resistant strains on tributyrin plates, performed second electroporation based on single-displayed ROL on GS115/KpRS recombinants and single-displayed CRL1 on GS115/ZCS recombinants and obtained an ROL/CRL1 codisplay on P. pastoris GS115 surfaces. The maximum activity of the codisplaying GS115/pRCS recombinant was 470.59 U/g dried cells, which was 3.9-fold and 1.3-fold higher than that of single-displayed ROL and CRL1, respectively. When self-immobilized lipases were used as whole-cell catalysts, the rate of methyl ester production from GS115/pRCS harboring ROL and CRL1 was 1.4-fold higher than that obtained with single-displayed ROL. Therefore, biodiesel catalysis by synergetic codisplayed enzymes is an alternative biodiesel production strategy.

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