Effects of viral promoters, the Woodchuck hepatitis post-transcriptional regulatory element, and weakened antibiotic resistance markers on transgene expression in Chinese hamster ovary cells

2020 ◽  
Vol 94 ◽  
pp. 258-265
Author(s):  
Wen Wang ◽  
Xiao Guo ◽  
Si-Jia Chen ◽  
Tian-Yun Wang ◽  
Fang Wang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Chinese Hamster Ovary ◽  
Transgene Expression ◽  
Regulatory Element ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Transcriptional Regulatory ◽  
Viral Promoters ◽  
Resistance Markers

scholarly journals Linkage of the MBG locus to another functionally hemizygous gene locus (IDH2) on chromosome Z3 in Chinese hamster ovary cells.

1985 ◽  
Vol 5 (1) ◽  
pp. 109-113 ◽  
Author(s):  
G M Adair ◽  
M J Siciliano
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Gene Locus ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Chromosome 8 ◽  
Chromosome 2 ◽  
Ovary Cells ◽  
Resistance Markers ◽  
Linkage Relationships

To gain insight into the nature of hemizygosity in Chinese hamster ovary (CHO) cells and the mechanisms by which it has arisen, we are attempting to map and determine linkage relationships for as many hemizygous loci as possible. In this study, we have shown by segregation analysis of intraspecific somatic cell hybrids that the hemizygous gene locus associated with resistance to methylglyoxalbisguanyl hydrazone (MBG) in CHO cells is linked to the hemizygous IDH2 locus on chromosome Z3. Nine of the ten autosomal hemizygous gene loci that have been mapped to date in CHO cells are clustered on three chromosomes, with five such markers on chromosome 2, two on chromosome 8, and now two on the Z3 chromosome. With the mapping of MBG to the Z3 chromosome, selectable drug resistance markers are now available on eight different CHO chromosomes.

Distance effect of matrix attachment regions on transgene expression in stably transfected Chinese hamster ovary cells

2014 ◽  
Vol 36 (10) ◽  
pp. 1937-1943 ◽  
Author(s):  
Jun-He Zhang ◽  
Xiao-Yin Wang ◽  
Tian-Yun Wang ◽  
Fang Wang ◽  
Wei-Hua Dong ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Transgene Expression ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Distance Effect ◽  
Matrix Attachment Regions ◽  
Ovary Cells

Identification of a novel endogenous regulatory element in Chinese hamster ovary cells by promoter trap

2013 ◽  
Vol 167 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Janice Chen ◽  
Jonathon Haverty ◽  
Liang Deng ◽  
Guanghua Li ◽  
Ping Qiu ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Regulatory Element ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Promoter Trap
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