The content and distribution of organic and inorganic carbon along the profile in the soils of the Rostov agglomeration are considered. The results obtained on a TOC-L CPN Shimadzu carbon analyzer are evaluated using Student's t-test. The groups of some horizons of native soils AU rz, AU, BCA, C, as well as their buried analogues [AU], [BCA], [C] were compared. The analysis of the urbic horizons was carried out taking into account their particle size distribution, previously dividing into horizons clusters - heavy URh and light URl. In the [AU] horizon of soils buried under the anthropogenic stratum, a statistically significant decrease in the organic carbon content is observed, compared to the AU horizon of natural analogues. The middle part of the profile - the BCA and [BCA] horizons - is characterized by the greatest similarity in carbon content, both organic and inorganic, which suggests the lowest anthropogenic impact on these horizons. Significant differences in the inorganic carbon content in the comparison pair - horizons C and [C] are shown, and its content is higher in natural analogues (horizons C). The data analysis of the urbic diagnostic horizons did not reveal any significant regularities because of the high degree of variation of this indicator due to the genesis peculiarities of the anthropogenically transformed stratum.