Exhaled nitric oxide is reduced upon chronic intermittent hypoxia exposure in well-acclimatized mine workers

2011 ◽  
Vol 175 (2) ◽  
pp. 261-264 ◽  
Author(s):  
Denis Vinnikov ◽  
Nurlan Brimkulov ◽  
Rupert Redding-Jones ◽  
Kalysbubu Jumabaeva
Keyword(s):  
Nitric Oxide ◽  
Intermittent Hypoxia ◽  
Exhaled Nitric Oxide ◽  
Chronic Intermittent Hypoxia ◽  
Hypoxia Exposure ◽  
Mine Workers

scholarly journals Effect of superoxide anion scavenger on rat hearts with chronic intermittent hypoxia

2016 ◽  
Vol 120 (8) ◽  
pp. 982-990 ◽  
Author(s):  
Peiying Pai ◽  
Ching Jung Lai ◽  
Ching-Yuang Lin ◽  
Yi-Fan Liou ◽  
Chih-Yang Huang ◽  
...  
Keyword(s):  
Sleep Apnea ◽  
Superoxide Anion ◽  
Intermittent Hypoxia ◽  
Caspase 3 ◽  
Fas Ligand ◽  
Left Ventricular ◽  
Chronic Intermittent Hypoxia ◽  
Apoptotic Pathway ◽  
Hypoxia Exposure ◽  
Survival Pathway

Only very limited information regarding the protective effects of the superoxide anion scavenger on chronic intermittent hypoxia-induced cardiac apoptosis is available. The purpose of this study is to evaluate the effects of the superoxide anion scavenger on cardiac apoptotic and prosurvival pathways in rats with sleep apnea. Forty-two Sprague-Dawley rats were divided into three groups, rats with normoxic exposure (Control, 21% O2, 1 mo), rats with chronic intermittent hypoxia exposure (Hypoxia, 3-7% O2 vs. 21% O2 per 40 s cycle, 8 h per day, 1 mo), and rats with pretreatment of the superoxide anion scavenger and chronic intermittent hypoxia exposure (Hypoxia-O2−-Scavenger, MnTMPyP pentachloride, 1 mg/kg ip per day; 3–7% O2 vs. 21% O2 per 40 s cycle, 8 h per day, 1 mo) at 5–6 mo of age. After 1 mo, the protein levels and apoptotic cells of excised hearts from three groups were measured by Western blotting and terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assay. The superoxide anion scavenger decreased hypoxia-induced myocardial architecture abnormalities, left ventricular hypertrophy, and TUNEL-positive apoptosis. The superoxide anion scavenger decreased hypoxia-induced Fas ligand, Fas death receptors, Fas-associated death domain (FADD), activated caspase-8, and activated caspase-3 (Fas-dependent apoptotic pathway) as well as Bad, activated caspase-9 and activated caspase-3 (mitochondria-dependent apoptotic pathway), endonuclease G (EndoG), apoptosis-inducing factor (AIF), and TUNEL-positive apoptosis. The superoxide anion scavenger increased IGF-1, IGF-1R, p-PI3k, p-Akt, p-Bad, Bcl-2, and Bcl-xL (survival pathway). Our findings imply that the superoxide anion scavenger might prevent cardiac Fas-mediated and mitochondrial-mediated apoptosis and enhance the IGF-1-related survival pathway in chronic intermittent hypoxia. The superoxide anion scavenger may prevent chronic sleep apnea-enhanced cardiac apoptotic pathways and enhances cardiac survival pathways.

scholarly journals Acclimatization to chronic intermittent hypoxia in mine workers: a challenge to mountain medicine in Chile

2013 ◽  
Vol 46 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Jorge G Farias ◽  
Daniel Jimenez ◽  
Jorge Osorio ◽  
Andrea B Zepeda ◽  
Carolina A Figueroa ◽  
...  
Keyword(s):  
Intermittent Hypoxia ◽  
Chronic Intermittent Hypoxia ◽  
Mine Workers

scholarly journals Nitric Oxide and Superoxide Anion Balance in Rats Exposed to Chronic and Long Term Intermittent Hypoxia

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Patricia Siques ◽  
Ángel Luis López de Pablo ◽  
Julio Brito ◽  
Silvia M. Arribas ◽  
Karen Flores ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Pulmonary Artery ◽  
Nadph Oxidase ◽  
Superoxide Anion ◽  
Intermittent Hypoxia ◽  
Ventricular Hypertrophy ◽  
Chronic Intermittent Hypoxia ◽  
Enos Expression ◽  
Fluorescent Indicators

Work at high altitude in shifts exposes humans to a new form of chronic intermittent hypoxia, with still unknown health consequences. We have established a rat model resembling this situation, which develops a milder form of right ventricular hypertrophy and pulmonary artery remodelling compared to continuous chronic exposure. We aimed to compare the alterations in pulmonary artery nitric oxide (NO) availability induced by these forms of hypoxia and the mechanisms implicated. Rats were exposed for 46 days to normoxia or hypobaric hypoxia, either continuous (CH) or intermittent (2 day shifts, CIH2x2), and assessed: NO and superoxide anion availability (fluorescent indicators and confocal microscopy); expression of phosphorylated endothelial NO synthase (eNOS), NADPH-oxidase (p22phox), and 3-nitrotyrosine (western blotting); and NADPH-oxidase location (immunohistochemistry). Compared to normoxia, (1) NO availability was reduced and superoxide anion was increased in both hypoxic groups, with a larger effect in CH, (2) eNOS expression was only reduced in CH, (3) NADPH-oxidase was similarly increased in both hypoxic groups, and (4) 3-nitrotyrosine was increased to a larger extent in CH. In conclusion, intermittent hypoxia reduces NO availability through superoxide anion destruction, without reducing its synthesis, while continuous hypoxia affects both, producing larger nitrosative damage which could be related to the more severe cardiovascular alterations.

scholarly journals Alpha Lipoic Acid Improves Endothelial Function and Oxidative Stress in Mice Exposed to Chronic Intermittent Hypoxia

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Mohammad Badran ◽  
Bisher Abuyassin ◽  
Saeid Golbidi ◽  
Najib Ayas ◽  
Ismail Laher
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Endothelial Dysfunction ◽  
Endothelial Function ◽  
Intermittent Hypoxia ◽  
Chronic Intermittent Hypoxia ◽  
Alpha Lipoic Acid ◽  
Systemic Oxidative Stress ◽  
Enos Uncoupling ◽  
And Oxidative Stress

Objective. Obstructive sleep apnea (OSA) is characterized by recurrent airway collapse that causes chronic intermittent hypoxia (CIH). OSA is associated with systemic inflammation and oxidative stress resulting in endothelial dysfunction and cardiovascular disease (CVD). Alpha lipoic acid (ALA) is a potent antioxidant with anti-inflammatory properties. We hypothesized that dietary ALA can improve endothelial function of mice exposed to CIH. Methods. Mice were exposed to either CIH or intermittent air (IA) and treated with dietary ALA (0.2% w/w) or a regular chow diet for 8 weeks. Endothelial function, endothelial nitric oxide (eNOS) uncoupling, systemic oxidative stress, systemic inflammation, aortic expression of inflammatory cytokines, and antioxidant enzymes were measured after 8 weeks. Results. Mice exposed to CIH exhibited endothelial dysfunction accompanied by systemic oxidative stress and inflammation as well as increased aortic expression of inflammatory cytokines. Furthermore, CIH led to eNOS uncoupling. Treatment with dietary ALA reversed endothelial dysfunction in mice exposed to CIH, lowered systemic oxidative stress and inflammation, prevented the increases of inflammatory cytokine gene expression, increased the expression of antioxidant enzymes, and preserved eNOS in a coupled state. Conclusion. ALA attenuates endothelial dysfunction by preventing oxidative stress and inflammation and restoring nitric oxide bioavailability in mice exposed to CIH. Our data suggests the potential beneficial use of ALA as adjunctive therapy in OSA.

scholarly journals Potential Role of mRNAs and LncRNAs in Chronic Intermittent Hypoxia Exposure-Aggravated Atherosclerosis

2020 ◽  
Vol 11 ◽  
Author(s):  
Jing Zhang ◽  
Chaowei Hu ◽  
Xiaolu Jiao ◽  
Yunyun Yang ◽  
Juan Li ◽  
...  
Keyword(s):  
Intermittent Hypoxia ◽  
Potential Role ◽  
Chronic Intermittent Hypoxia ◽  
Hypoxia Exposure
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