Effect of whole cigarette smoke exposure on MUC5AC mucin hyperproduction in primary cultures of human bronchial epithelial cells at air-liquid interface of COPD, smokers and control patients

2014 ◽  
Vol 31 (7) ◽  
pp. 652
Author(s):  
J. Charriot ◽  
I. Vachier ◽  
A. Fort-Petit ◽  
A. Bourdin
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Primary Cultures ◽  
Bronchial Epithelial Cells ◽  
Liquid Interface ◽  
Cigarette Smoke Exposure ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Air Liquid Interface ◽  
And Control

scholarly journals Sub-ohm vaping increases the levels of carbonyls, is cytotoxic, and alters gene expression in human bronchial epithelial cells exposed at the air–liquid interface

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Alexandra Noël ◽  
Ekhtear Hossain ◽  
Zakia Perveen ◽  
Hasan Zaman ◽  
Arthur L. Penn
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Liquid Interface ◽  
Third Generation ◽  
Human Bronchial Epithelial Cells ◽  
Aerosol Composition ◽  
Exposure System ◽  
Bronchial Epithelial ◽  
Air Liquid Interface

Abstract Background Exposure to electronic-cigarette (e-cig) aerosols induces potentially fatal e-cig or vaping-associated lung injury (EVALI). The cellular and molecular mechanisms underlying these effects, however, are unknown. We used an air–liquid interface (ALI) in vitro model to determine the influence of two design characteristics of third-generation tank-style e-cig devices—resistance and voltage—on (1) e-cig aerosol composition and (2) cellular toxicity. Methods Human bronchial epithelial cells (H292) were exposed to either butter-flavored or cinnamon-flavored e-cig aerosols at the ALI in a Vitrocell exposure system connected to a third-generation e-cig device. Exposures were conducted following a standard vaping topography profile for 2 h per day, for 1 or 3 consecutive days. 24 h after ALI exposures cellular and molecular outcomes were assessed. Results We found that butter-flavored e-cig aerosol produced under ‘sub-ohm’ conditions (< 0.5 Ω) contains high levels of carbonyls (7–15 μg/puff), including formaldehyde, acetaldehyde and acrolein. E-cig aerosol produced under regular vaping conditions (resistance > 1 Ω and voltage > 4.5 V), contains lower carbonyl levels (< 2 μg/puff). We also found that the levels of carbonyls produced in the cinnamon-flavored e-cig aerosols were much lower than that of the butter-flavored aerosols. H292 cells exposed to butter-flavored or cinnamon-flavored e-cig aerosol at the ALI under ‘sub-ohm’ conditions for 1 or 3 days displayed significant cytotoxicity, decreased tight junction integrity, increased reactive oxygen species production, and dysregulated gene expression related to biotransformation, inflammation and oxidative stress (OS). Additionally, the cinnamon-flavored e-cig aerosol induced pro-oxidant effects as evidenced by increases in 8-hydroxy-2-deoxyguanosine protein levels. Moreover, we confirmed the involvement of OS as a toxicity process for cinnamon-flavored e-cig aerosol by pre-treating the cells with N-acetyl cysteine (NAC), an antioxidant that prevented the cells from the OS-mediated damage induced by the e-cig aerosol. Conclusion The production of high levels of carbonyls may be flavor specific. Overall, inhaling e-cig aerosols produced under ‘sub-ohm’ conditions is detrimental to lung epithelial cells, potentially via mechanisms associated with OS. This information could help policymakers take the necessary steps to prevent the manufacturing of sub-ohm atomizers for e-cig devices.

scholarly journals Short- and Long- Term Effects of Cigarette Smoke Exposure on Glutathione Homeostasis in Human Bronchial Epithelial Cells

2013 ◽  
Vol 32 (7) ◽  
pp. 129-145 ◽  
Author(s):  
Claudia Bazzini ◽  
Valeria Rossetti ◽  
Davide Antonio Civello ◽  
Francesca Sassone ◽  
Valeria Vezzoli ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Bronchial Epithelial Cells ◽  
Cigarette Smoke Exposure ◽  
Long Term Effects ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Short And Long Term ◽  
Glutathione Homeostasis

scholarly journals Growth of human bronchial epithelial cells at an air-liquid interface alters the response to particle exposure

2013 ◽  
Vol 10 (1) ◽  
pp. 25 ◽  
Author(s):  
Andrew J Ghio ◽  
Lisa A Dailey ◽  
Joleen M Soukup ◽  
Jacqueline Stonehuerner ◽  
Judy H Richards ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Liquid Interface ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Particle Exposure ◽  
Air Liquid Interface
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