Outbreak of acute bovine viral diarrhea in Brazilian beef cattle: Clinicopathological findings and molecular characterization of a wild-type BVDV strain subtype 1b

AbstractIn 2019, diarrhea cases occurred on cattle farms in Qionglai and Guang'an, Sichuan Province. Two out of 20 (10%) serum and nasal swab samples were positive when tested using a bovine viral diarrhea virus (BVDV) antigen-capture ELISA kit. Two non-cytopathic strains of BVDV were isolated and named QL1903 and GA190608, respectively. The nucleotide sequences of the genomes of the two isolates were 89.52% identical. Phylogenetic analysis based on the 5'-UTR sequence revealed that the BVDV isolate QL1903 belonged to BVDV subtype 1b, whereas isolate GA190608 clustered with strains HN1814, EN-19, and BJ09_26 in a separate branch, which has tentatively been classified as a new genetic subtype, "1v".

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Molecular Characterization of the CytochromebGene andIn VitroAtovaquone Susceptibility of Plasmodium falciparum Isolates from Kenya

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.03956-14 ◽

2015 ◽

Vol 59 (3) ◽

pp. 1818-1821 ◽

Cited By ~ 6

Author(s):

Luicer A. Ingasia ◽

Hoseah M. Akala ◽

Mabel O. Imbuga ◽

Benjamin H. Opot ◽

Fredrick L. Eyase ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Genetic Polymorphism ◽

Molecular Characterization ◽

Mutant Allele ◽

Inhibitory Concentration ◽

Wild Type Allele ◽

Wild Type ◽

Western Kenya ◽

Content Type

ABSTRACTThe prevalence of a genetic polymorphism(s) at codon 268 in the cytochromebgene, which is associated with failure of atovaquone-proguanil treatment, was analyzed in 227Plasmodium falciparumparasites from western Kenya. The prevalence of the wild-type allele was 63%, and that of the Y268S (denoting a Y-to-S change at position 268) mutant allele was 2%. There were no pure Y268C or Y268N mutant alleles, only mixtures of a mutant allele(s) with the wild type. There was a correlation between parasite 50% inhibitory concentration (IC50) and parasite genetic polymorphism; mutant alleles had higher IC50s than the wild type.

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Trans-splicing as a Novel Mechanism to Explain Interallelic Complementation in Drosophila

Genetics ◽

10.1093/genetics/160.4.1481 ◽

2002 ◽

Vol 160 (4) ◽

pp. 1481-1487 ◽

Cited By ~ 2

Author(s):

Fabien Mongelard ◽

Mariano Labrador ◽

Ellen M Baxter ◽

Tatiana I Gerasimova ◽

Victor G Corces

Keyword(s):

Molecular Characterization ◽

Wild Type ◽

Functional Protein ◽

Gypsy Insulator ◽

Type Function ◽

Homologous Chromosomes ◽

Trans Splicing ◽

Interallelic Complementation

AbstractTwo mutant alleles of the same gene, each located in one of the two homologous chromosomes, may in some instances restore the wild-type function of the gene. This is the case with certain combinations of mutant alleles in the mod(mdg4) gene. This gene encodes several different proteins, including Mod(mdg4)2.2, a component of the gypsy insulator. This protein is encoded by two separate transcription units that can be combined in a trans-splicing reaction to form the mature Mod(mdg4)2.2-encoding RNA. Molecular characterization of complementing alleles shows that they affect the two different transcription units. Flies homozygous for each allele are missing the Mod(mdg4)2.2 protein, whereas wild-type trans-heterozygotes are able to synthesize almost normal levels of the Mod(mdg4)2.2 product. This protein is functional as judged by its ability to form a functional insulator complex. The results suggest that the interallelic complementation in the mod(mdg4) gene is a consequence of trans-splicing between two different mutant transcripts. A conclusion from this observation is that the trans-splicing reaction that takes place between transcripts produced on two different mutant chromosomes ensures wild-type levels of functional protein.

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