RAPD analysis and sequencing of ITS1/5.8S rRNA/ITS2 and Fe-hydrogenase as tools for genetic classification of potentially pathogenic isolates of Trichomonas gallinae

2016 ◽  
Vol 107 ◽  
pp. 182-189 ◽  
Author(s):  
José Sansano-Maestre ◽  
María del Carmen Martínez-Herrero ◽  
María Magdalena Garijo-Toledo ◽  
María Teresa Gómez-Muñoz
2021 ◽  
Author(s):  
Sonoko Matsumoto ◽  
Kenta Watanabe ◽  
Akiko Imamura ◽  
Masato Tachibana ◽  
Takashi Shimizu ◽  
...  

Abstract Paramecium spp. is types of free-living protists that live in freshwater environments. They are ciliates with high motility and phagocytosis and have been used to analyze cell motility and as a host model for pathogens. Besides such biological characteristics, apart from the usual morphological and genetic classification of species, the existence of taxonomies (such as syngens) and mating types related to Paramecium’s unique reproduction is known. In this study, we attempted to develop a simple method to identify Paramecium strains, which are difficult to distinguish morphologically, using random amplified polymorphic DNA (RAPD) analysis. Consequently, we can observe strain-specific band patterns. We also confirm that the presence of endosymbiotic Chlorella cells affects the band pattern of P. bursaria. Furthermore, the results of the RAPD analysis using several P. caudatum strains with different syngens show that it is possible to detect a band specific to a certain syngen. By improving the reaction conditions and random primers, based on the results of this study, RAPD analysis can be applied to the identification of Paramecium strains and their syngen confirmation tests.


2021 ◽  
Author(s):  
Yoshikage Inoue ◽  
Nobuyuki Kakiuchi ◽  
Kenichi Yoshida ◽  
Yasuhito Nanya ◽  
Yusuke Shiozawa ◽  
...  

Author(s):  

Rice is a world-famous cereal food divided into pigmented and non-pigmented rice. Pigmented rice is popular as healthier food than non-pigmented rice due to its potency as an antioxidant. Nevertheless, the potential of pigmented rice has not been widely studied. Indonesian selected pigmented rice protein’s antioxidant potential and the non-protein compound were in-vitro studied. The antioxidant potencies were evaluated by extracting fresh seeds of nine pigmented rice (Aek Sibundong, Beureum Taleus, Gogo Niti-2, Lamongan-1, Merah SP, Merah Wangi, Mota, Ketan Hitam-2, and Super Manggis) and non-pigmented rice (IR-64) as control. Various free radical scavenging methods to determine the antioxidant activity (ABTS•+, DPPH•, OH• and O2-) were conducted. Meanwhile, the genetic classification was performed by a simple sequence repeat (SSR) marker to determine the relationship between varieties. The results showed that protein of Ketan Hitam-2 had the highest ABTS•+ radical scavenging (98.06%), followed by Beureum Taleus (42.54%). Ketan Hitam-2 protein also showed the highest OH• and O2- activities (43.49% and 6.02%, respectively). The highest DPPH• potency of the non-protein compounds also shown by Ketan Hitam-2 (32.23%) with the activity of OH• and O2- (20.63% and 14.56%, respectively). These results showed that Ketan Hitam-2 has the highest potency as an antioxidant, which could be recommended as a nutraceuticals compound.


2005 ◽  
Vol 4 (4) ◽  
pp. 305-309 ◽  
Author(s):  
Kassinee Sitthiwong . ◽  
Toshiyuki Matsui . ◽  
Sutevee Sukprakarn .

2018 ◽  
Vol 20 (suppl_5) ◽  
pp. v346-v346
Author(s):  
Laurent James Livermore ◽  
Martin Isabelle ◽  
Ian Bell ◽  
Puneet Plaha ◽  
Claire Vallance ◽  
...  

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