scholarly journals Optimization of in vitro propagation of Coleonema album, a highly utilized medicinal and ornamental plant

2014 ◽  
Vol 94 ◽  
pp. 9-13 ◽  
Author(s):  
O.O. Fajinmi ◽  
S.O. Amoo ◽  
J.F. Finnie ◽  
J. Van Staden
Keyword(s):  
In Vitro Propagation ◽  
Ornamental Plant

In vitro propagation of medicinal and ornamental plant Lysimachia davurica

2017 ◽  
Vol 82 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Yanni Zhang ◽  
◽  
Dongling Sun ◽  
Shangchun Hu ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Ornamental Plant

scholarly journals Effects of Hormonal and Basal Nutrient Medium on In vitro Regeneration of an Ornamental Plant - Muscari armeniacum Leichtlin. ex Baker

2013 ◽  
Vol 22 (2) ◽  
pp. 113-126 ◽  
Author(s):  
Mustafa Abul Kalam Azad ◽  
Muhammad Nurul Amin
Keyword(s):  
In Vitro Propagation ◽  
Average Length ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Leaf Sheath ◽  
Ornamental Plant ◽  
Garden Soil ◽  
Ex Vitro ◽  
Auxin Concentration

In vitro propagation system has been developed for an important ornamental and medicinal plant, Muscari armeniacum Leichtil. ex Bak. A range of a cytokinin and auxin concentration has been investigated for axillary bulblet proliferation, and direct and indirect adventitious bulblet regeneration from the explants whole bulb, one fourth part of bulb, bulb-scale of ex vitro (field grown mature bulb), and only leaf-sheath explants of in vitro grown bulblet. Axillary bulblet regeneration occurred on MS containing 2.0 - 8.0 ?M BAP or Kn. Direct adventitious bulblets were induced successfully on MS basal medium supplemented with various concentrations of BAP or Kn (1.0 - 4.0 ?M) in combi-nation of either NAA, IBA, or 2,4-D (0.5 - 4.0 ?M). The maximum frequency of adventitious bulblets regeneration occurred from both bulb-scale and leaf-sheath explants on MS with 4.0 ?M BAP and 2.0 ?M NAA, IBA, or 2,4-D. The highest frequency (95.5%) of indirect adventitious bulblets was obtained from in vitro grown leaf-sheathderived callus on MS containing 4.0 ?M BAP with 1.0 ?M 2,4-D whereas, highest number (80.2) and average length (55.5 cm) of bulblets were obtained on MS supplemented with 4.0 ?M BAP and 1.0 ?M NAA. In vitro grown bullets was rooted successfully on MS with 0.5 - 4.0 ?M of IBA, NAA, or IAA. The rooted bulblets were transferred to garden soil and successfully established under ex vitro environment. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14200 Plant Tissue Cult. & Biotech. 22(2): 113-126, 2012 (December)

In vitro propagation of cambuci (Campomanesia phaea): An endangered exotic fruit and ornamental plant from Brazilian Atlantic Forest

2021 ◽  
Author(s):  
Christian Aparecido Demétrio ◽  
Jessica Fernanda de Oliveira Jacob ◽  
Guilherme Bovi Ambrosano ◽  
Ênio Tiago de Oliveira ◽  
Paulo Hercílio Viegas Rodrigues
Keyword(s):  
Atlantic Forest ◽  
In Vitro Propagation ◽  
Ornamental Plant ◽  
Brazilian Atlantic Forest

scholarly journals Effects of Plant Growth Regulators on in vitro Propagation of Economically Important Ornamental Plant Rosa hybrida L.

2020 ◽  
Vol 9 (2) ◽  
pp. 227-233
Author(s):  
Diwakar Aggarwal ◽  
Sushil Kumar Upadhyay ◽  
Kuldeep Kumar ◽  
Nirmala Sehrawat ◽  
Hardeep Singh Tuli ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Ornamental Plant ◽  
Rosa Hybrida

Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application

2021 ◽  
Vol 6 (2) ◽  
pp. 284-294
Author(s):  
Lily Ismaini ◽  
Intani Quarta Lailaty ◽  
Muhammad Efendi
Keyword(s):  
Analysis Of Variance ◽  
In Vitro Propagation ◽  
Growth Response ◽  
Culture Media ◽  
Ornamental Plant ◽  
Botanic Gardens ◽  
Growth Responses ◽  
Significance Level ◽  
Shoot Number

Three species of Begonias endemic to Java and Sumatra, namely Begonia leuserensis, Begonia atricha and Begonia scottii, were conserved in Cibodas Botanic Gardens as sources of germplasm for ornamental plant and/or medicines. However, the information on efficient hormones concentration and their culture media application through an in vitro propagation effort is still limited. Therefore, this study aimed to explain the growth response of three species of Begonias using various hormones concentrations and culture media through in vitro propagation. The culture media using Murashige & Skoog (MS) media that combinedwith 6-Benzyladenine (BA) dan Thidiazuron (TDZ) hormones in different concentrations i.e. 0.5 mg/L, 1 mg/L, 2 mg/L, and 3 mg/L. Observation parameter included shoot number, plantlets height, and leaves number. The data were analyzed using analysis of variance (ANOVA) with the F test at a 5% significance level. The results showed that three species of Begonias were observed to have different growth responses in the combination of MS+BA and MS+TDZ media. The combination of MS+TDZ media produces more shoots number, while the combination of MS+BA media influenced higher in leaves number. A concentration of 0.5 mg/L of hormone showed a good regeneration, therefore were recommended for in vitro propagation of Begonia species.

scholarly journals Rapid In Vitro Propagation of Aruncus `Misty Lace', a New Heat-tolerant, Dwarf, Hybrid Goatsbeard

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 848A-848
Author(s):  
Hazel Y. Wetzstein* ◽  
Allan M. Armitage ◽  
Gwen N. Hirsch ◽  
Stephanie L. Anderson
Keyword(s):  
Plant Material ◽  
In Vitro Propagation ◽  
Shoot Organogenesis ◽  
Ornamental Plant ◽  
Naphthalene Acetic Acid ◽  
Ex Vitro ◽  
Regenerated Plants ◽  
Half Strength ◽  
Propagation Methods

Tissue culture is a useful means to clonally propagate new ornamental plant selections, particularly when plant material is limited and/or conventional propagation methods are ineffective. An efficient in vitro multiplication protocol was established to propagate a new goatsbeard hybrid (Aruncus dioicus, × A. aethusifolia). The hybrid is of interest because it exhibits a dwarf habit, delicate white flower panicles and fern-like leaves, yet is tolerant to heat and humidity. Experiments were conducted to evaluate explant type (nodes, stems, leaves, and floral parts), disinfestation procedures, and media formulations including varying concentrations of 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA). Rapid plant regeneration was obtained with a shoot organogenesis system using a half strength Murashige and Skoog medium supplemented with 4.4 μmol BAP, 0.54 μmol NAA, 30 g·L-1 sucrose, and 3.0 g·L-1 GelGro. Studies compared the performance and yield of plants rooted using different in vitro and ex vitro methods. Ex vitro rooting of shoots during greenhouse acclimatization under mist was most effective. Regenerated plants exhibited uniform and rapid growth, and performed well in greenhouse and field evaluations.

scholarly journals In Vitro Propagation of Alexandrian Laurel (Danae racemosa L. Moench), a Valuable Ornamental Plant

HortScience ◽  
2013 ◽  
Vol 48 (10) ◽  
pp. 1301-1303
Author(s):  
Xiuli Shen ◽  
Guochen Yang ◽  
Zhongge (Cindy) Lu
Keyword(s):  
Morphological Variation ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Ornamental Plant ◽  
Efficient Production ◽  
Ms Medium ◽  
Culture Initiation ◽  
Shoot Number ◽  
Alternative Means

To overcome the limitations of traditional propagation, this research was initiated to develop an alternative means for efficient production of Alexandrian laurel (Danae racemosa L. Moench). An in vitro propagation protocol has been developed for Danae racemosa L. Moench using seeds as a source of material for culture initiation. Seedlings were produced after seeds were cultured for 3 month on MS (Murashige and Skoog, 1962) medium. Shoot multiplication occurred on MS medium with or without 6-benzylaminopurine (BAP) with 100% multiplication percentage. However, shoot number was significantly increased from an average of 2.8 to more than six with the addition of 5 or 25 μM BAP. Among two indole-3-butyric acid (IBA) treatments tested for rooting of seedlings, incorporation of 5 μM IBA in MS medium significantly increased rooting percentage to 86.4% compared with 71.2% without IBA. The greatest number of roots (three) was produced by 5-minute IBA pulse. However, both IBA treatments significantly reduced root length. The longest root (12.8 mm) was observed on MS medium without any IBA treatment and the shortest (6.1 mm) was produced by IBA pulse. In vitro-propagated plantlets grew well after transfer to a substrate of peat and pine bark (1:1) in the greenhouse. No morphological variation was observed.

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