In vitro insecticidal effect of commercial fatty acids, ß-sitosterol and rutin against the sugarcane aphid, Melanaphis sacchari Zehntner (Hemiptera: Aphididae)

The sugarcane aphid, Melanaphis sacchari Zehntner (Hemiptera: Aphididae), is the main pest of sorghum, Sorghum bicolor L. Moench (Poaceae), in Mexico. To control this insect, farmers currently use synthetic chemical insecticides, which are toxic to humans and biodiversity. However, natural products are a promising potential source of safer alternative means to control different agricultural pests. The main objective of this study was to evaluate the insecticidal effect of contact by fumigation of pure molecules of four commercial fatty acids (palmitic, stearic, pentadecanoic and linoleic acids), the phytosterol ß -sitosterol, and the flavonoid rutin. The results showed that fatty acids were the most effective against M. sacchari ; the highest mortality rate (85%) was produced by linoleic acid and the LC 50 was 1,181 ppm, followed by stearic and palmitic acids with mortality percentages of 74 and 63%, respectively, at a concentration of 2,500 ppm at 72 h. The positive control, imidacloprid, had 100% mortality in 24 h and the tween 20 negative control exhibited 4% mortality in 72 h. Our results show that commercial fatty acids are effective against adults of M. sacchari , and can be considered an environmentally friendly alternative to the frequent use of synthetic chemical insecticides.

Stress-Induced Despair Behavior Develops Independently of the AHR-RORgt Axis in CD4+ cells

Current treatments for major depressive disorder are limited to neuropharmacological approaches and are ineffective for large numbers of patients. Recently, alternative means have been explored to understand the etiology of depression. Specifically, changes in the microbiome and immune system have been observed in both clinical settings and in mouse models. As such, microbial supplements and probiotics have become a target for potential therapeutics. A current hypothesis for the mechanism of action of these supplements is via the aryl hydrocarbon receptor’s (AHR) modulation of the T helper 17 cell (Th17) and T regulatory cell axis. As inflammatory RORgt+ CD4+ Th17 T cells and their primary cytokine IL-17 have been implicated in the development of stress-induced depression, the connection between stress, the AHR, Th17s and depression remains critical to disease understanding. Here, we utilize genetic knockouts to examine the role of the microbial sensor AHR in the development of stress induced despair behavior. We observe an AHR-independent increase in gut-associated Th17s in stressed mice, indicating that AHR is not responsible for this communication. Further, we utilized a CD4-specific Rorc knockout line to disrupt the production of Th17s. Mice lacking Rorc induced IL-17 did not show any differences in behavior from controls before or after stress. Finally, we utilize an unsupervised machine learning system to examine minute differences in behavior that could not be observed in traditional behavioral assays. Our data demonstrate that neither CD4 specific Ahr nor Rorc are necessary for the development of stress-induced anxiety-or depressive-like behaviors. These data suggest that research approaches should focus on other sources or sites of IL-17 production in stress-induced depression.

Implementing the E-Family Expert Model through a Legal Framework for Online Dispute Resolution

With the protracted Covid-19 pandemic ravaging the whole world with emergence of new virulent variants, there has been a massive backlash on the family institutions, particularly Muslim families across the world. Some of the effects of the protracted pandemic include domestic violence, increase in the rate of divorce and death of spouses which have triggered some unique nature of family disputes. With the restrictions in movement and physical distancing measures in place, one would wonder whether there could be an alternative means to resolve such budding family-related disputes. To address this issue, this paper examines the potential of the E-family Expert Model earlier designed by the authors in addressing such family-related disputes under the legal framework for online dispute resolution (ODR). The study adopts an analytical approach through qualitative research method to discuss the relevant issues with a view to finding a lasting solution to the dearth of avenues for family dispute resolution. One of the major findings of the study relates to the relevance of the Fourth Industrial Revolution in implementing the E-Family Expert model where the usual boundaries between the physical, digital and biological aspects of life are strategically blurred with the implementation of a family ODR platform. The study concludes that the E-family expert model could help in a great deal in addressing multitude of family-related disputes through an ODR platform while complying with the national legislative framework. This could be a veritable tool for the member states of the Organization of Islamic Cooperation (OIC) in addressing the increasing number of family disputes through a more formalized framework for adoption by its member states.

Effects of Indigenous and Non-Indigenous Arbuscular Mycorrhizal Fungi on Growth and Plant Nutrient Uptake by Terung Asam (Solanum lasiocarpum Dunal)

Terung asam (Solanum lasiocarpum Dunal) is a native fruit vegetable that is gaining interest as a commercial crop in Sarawak and Malaysia. Malaysia is covered by soils that are highly weathered, acidic, and low in fertility and depend on chemical fertilisers to promote good plant growth. Alternative means to reduce dependency on chemical fertiliser, for example arbuscular mycorrhiza fungi (AMF), must be sourced. Very few research on terung asam has been documented particularly on nutrients uptake. The objectives of this research were to investigate the effect of indigenous and non-indigenous AMF on nutrient uptake by terung asam. A greenhouse experiment was conducted consisting of three treatments namely control (T1), indigenous AMF (T2), non-indigenous AMF (T3). The treatments were arranged in a complete randomised design with four subsamples and four replicates. Thirty-day-old seedlings were transplanted and measured for their heights and stem diameters for 90 days. Fresh and dry shoot and root weights were taken during harvesting. Plant nutrient analyses were conducted using Kjeldahl method for total N, single ashing for P and single ashing and double acid for K, Ca and Mg. The results revealed the addition of AMF spores at 200 spores per pot, increased plant height by 13 to 33% and stem diameter by 5 to 25% and more leaves were retained by T3 plants at harvesting. T3 recorded higher fresh shoot (11.27%) and dry shoot (14.98%) as well as fresh root (23.67%) and dry root (22.77%) weights than T1 plants. Addition of AMF in treatments T2 and T3 promoted better nutrient uptake by aboveground and belowground biomasses particularly for K, Ca and Mg. T3 was superior in terms of the nutrient uptake for most nutrients. AMF used in T3 showed better results as the AMF spores were proven effective in promoting plant growth while AMF used in T2 were obtained from the field and untested. The findings of this study showed the potential of indigenous and non-indigenous AMF in promoting growth and nutrient uptake by terung asam plants.

A Prospective Study on the Efficacy and Safety of Kangaroo Mother Care as an Alternative Means of Transport of Preterm and Term Small-for-Gestational Age Infants

Background. The unavailability of transport incubators in resource-limited areas increases the risk for hypothermia in low birthweight neonates requiring transfer to another hospital. The kangaroo mother care (KMC) position may be a better alternative than swaddling the neonates during transport. Objective. To determine the safety and efficacy of KMC as an alternative means of transport of preterm and term small-for-gestational age (SGA) infants who need to be transferred to a higher level of care. Specifically, it aims to establish if KMC is safe and efficacious in terms of thermoregulation during inter-facility transfers. It also aims to determine the impact of transport distance from the referring hospital, age of gestation, sex, birthweight, and Apgar score on the efficacy of KMC in preventing hypothermia. Methods. We did a prospective, single-blinded, parallel-randomized controlled trial from September 2016 to October 2017 from a community-based primary care facility to a tertiary government hospital. We included newborn preterm infants and term SGA infants weighing 1200–1800 grams, delivered at health centers, district and provincial hospitals who needed to be transferred for a higher level of care. Outcomes included physiologic variables such as temperature, heart rate, respiratory rate. We conducted statistical analysis using t-test, risk ratio, and multiple regression analysis. Results. Thirty-one neonates were randomized to KMC transport (n=15) and conventional transport (swaddled) (n=16). Fifty percent of the swaddled infants developed hypothermia against none in the KMC infants. The risk of hypothermia was reduced by 93.75% in the neonates transported in KMC. The gestational age, birthweight, sex, Apgar scores and distance travelled had no confounding effect on the neonates’ temperature during transport. Conclusions. Kangaroo mother care transport is a safe, effective, and low-cost alternative in inter-facility neonatal transport especially in limited-resource areas.

Dejudicialization and Proceduralism Based on the Nature of Conflicts and According to the Brazilian Constitution

The objective of this paper is to show that Access to Justice is a broader concept than Access to the Judiciary. Apart from the movements for access to justice, it is the intention to prove that the Alternative Means of Dispute Resolution and the studies of Conflict Perspective are equally relevant, having in mind that they both defend a plurality of conflict processing institutions (state or not), based on the hypothesis that dejudicialization is an important way to strengthen institutions and promote economic and social development. Therefore, the deductive approach method was used in conjunction with the propositional-juridical method to demonstrate that the exhaustion of the state-owned model in solving conflicts shows that it is possible (and necessary) to develop the Proceduralism beyond the scope of the Judiciary, in order to institutionally expand forms of conflict resolution in civil society. From this, the concept of Proceduralism arises, interconnected with the due process and which is also suitable for the out-of-court ways of dispute resolution, in order to achieve adequate, effective and due process protection, so that pacification is carried out along the lines of constitutional guarantees, with constitutional procedurality also acting on the unjudicialized means of resolving conflicts.

Management of Oroantral Fistulae and Communications: Our Recommendations for Routine Practice

Oroantral communication (OAC) is one of the main complications of extracting antral or sinus teeth. OAC is a gap between the maxillary sinus and the oral cavity. When left untreated, it causes maxillary sinusitis and dramatically impairs the patient’s quality of life. Numerous surgical treatment techniques have been described, from mucosal techniques to using bone substitutes or very conservative alternative means. Many cases of recurrence after treatment have been reported, and the choice of the method adapted to the clinical situation remains challenging. Therefore, it is necessary to establish a correct diagnosis and choose the surgical technique best adapted to the case. This work is aimed at reviewing several techniques for the treatment of OAC and at describing our recommendations for use in daily practice for each of them through four clinical cases.

ForestScanner: A mobile application for measuring and mapping trees with LiDAR-equipped iPhone and iPad

Terrestrial laser scanning (TLS) is becoming increasingly popular as an alternative means to conventional forest inventory methods. By gauging the distances to multiple points on the surrounding object surfaces, TLS acquires 3D point clouds from which tree sizes and spatial distributions can be rapidly estimated. However, the high cost and specialized skills required for TLS have put it out of reach for many potential users. We here introduce ForestScanner, a free, mobile application that allows TLS-based forest inventories by means of iPhone or iPad with a built-in LiDAR sensor. ForestScanner does not require any manual analysis of 3D point clouds. As the user scans trees with an iPhone/iPad, ForestScanner estimates the stem diameters and spatial coordinates based on real-time instance segmentation and circle fitting. The users can visualize, check, and share the results of scanning in situ. By using ForestScanner, we measured the stem diameters and spatial coordinates of 672 trees within a 1 ha plot in 1 h 39 min with an iPhone and in 1 h 38 min with an iPad (diameter ≥5 cm; detection rate = 100%). ForestScanner reduced the person-hours required for measuring diameters to 25.7%, mapping trees to 9.3%, and doing both to 6.8% of the person-hours taken using a dimeter tape and a conventional surveying method. The diameters measured by ForestScanner and diameter tape were in good agreement; R2=0.963 for iPhone and R2=0.961 for iPad. ForestScanner and the conventional surveying system showed almost identical results for tree mapping (assessed by the spatial distances among trees within 0.04 ha subplots); Mantel R2=0.999 for both iPhone and iPad. Our results indicate that ForestScanner enables cost-, labor-, and time-efficient forest inventories. The application can increase the accessibility to TLS for people beyond specialists and enhance resource assessments and biodiversity monitoring in forests worldwide.