Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application

2021 ◽  
Vol 6 (2) ◽  
pp. 284-294
Author(s):  
Lily Ismaini ◽  
Intani Quarta Lailaty ◽  
Muhammad Efendi
Keyword(s):  
Analysis Of Variance ◽  
In Vitro Propagation ◽  
Growth Response ◽  
Culture Media ◽  
Ornamental Plant ◽  
Botanic Gardens ◽  
Growth Responses ◽  
Significance Level ◽  
Shoot Number

Three species of Begonias endemic to Java and Sumatra, namely Begonia leuserensis, Begonia atricha and Begonia scottii, were conserved in Cibodas Botanic Gardens as sources of germplasm for ornamental plant and/or medicines. However, the information on efficient hormones concentration and their culture media application through an in vitro propagation effort is still limited. Therefore, this study aimed to explain the growth response of three species of Begonias using various hormones concentrations and culture media through in vitro propagation. The culture media using Murashige & Skoog (MS) media that combinedwith 6-Benzyladenine (BA) dan Thidiazuron (TDZ) hormones in different concentrations i.e. 0.5 mg/L, 1 mg/L, 2 mg/L, and 3 mg/L. Observation parameter included shoot number, plantlets height, and leaves number. The data were analyzed using analysis of variance (ANOVA) with the F test at a 5% significance level. The results showed that three species of Begonias were observed to have different growth responses in the combination of MS+BA and MS+TDZ media. The combination of MS+TDZ media produces more shoots number, while the combination of MS+BA media influenced higher in leaves number. A concentration of 0.5 mg/L of hormone showed a good regeneration, therefore were recommended for in vitro propagation of Begonia species.

scholarly journals In Vitro Propagation of Alexandrian Laurel (Danae racemosa L. Moench), a Valuable Ornamental Plant

HortScience ◽  
2013 ◽  
Vol 48 (10) ◽  
pp. 1301-1303
Author(s):  
Xiuli Shen ◽  
Guochen Yang ◽  
Zhongge (Cindy) Lu
Keyword(s):  
Morphological Variation ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Ornamental Plant ◽  
Efficient Production ◽  
Ms Medium ◽  
Culture Initiation ◽  
Shoot Number ◽  
Alternative Means

To overcome the limitations of traditional propagation, this research was initiated to develop an alternative means for efficient production of Alexandrian laurel (Danae racemosa L. Moench). An in vitro propagation protocol has been developed for Danae racemosa L. Moench using seeds as a source of material for culture initiation. Seedlings were produced after seeds were cultured for 3 month on MS (Murashige and Skoog, 1962) medium. Shoot multiplication occurred on MS medium with or without 6-benzylaminopurine (BAP) with 100% multiplication percentage. However, shoot number was significantly increased from an average of 2.8 to more than six with the addition of 5 or 25 μM BAP. Among two indole-3-butyric acid (IBA) treatments tested for rooting of seedlings, incorporation of 5 μM IBA in MS medium significantly increased rooting percentage to 86.4% compared with 71.2% without IBA. The greatest number of roots (three) was produced by 5-minute IBA pulse. However, both IBA treatments significantly reduced root length. The longest root (12.8 mm) was observed on MS medium without any IBA treatment and the shortest (6.1 mm) was produced by IBA pulse. In vitro-propagated plantlets grew well after transfer to a substrate of peat and pine bark (1:1) in the greenhouse. No morphological variation was observed.

In vitro propagation of medicinal and ornamental plant Lysimachia davurica

2017 ◽  
Vol 82 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Yanni Zhang ◽  
◽  
Dongling Sun ◽  
Shangchun Hu ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Ornamental Plant

scholarly journals Micropropagation of Bacopa monnieri using Cyanobacterial Liquid Medium

1970 ◽  
Vol 20 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Meenakshi Banerjee ◽  
Priyanka Modi
Keyword(s):  
Plant Tissue ◽  
In Vitro Propagation ◽  
Liquid Culture ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Axillary Node ◽  
Bacopa Monnieri ◽  
Shoot Initiation ◽  
Aulosira Fertilissima

Hot extract of Aulosira fertilissima (cyanobacterium) added in different proportions to MS as a liquid culture media for the in vitro propagation of Bacopa monnieri (L.) Pennell. Maximum numbers of shoots were induced from axillary node in MS media (40 ml) + Aulosira extract (60 ml) and maximum shoot multiplication was observed when Kn (1.0 mg/l) was added in the shoot initiation media (mentioned above). Surprisingly rooting was also found to be best in the same combination of MS + cyanobacterial extract that was used for initiation and multiplication of shoots. On an average within a period of three subcultures (2 - 3 months) the nodal explants generated 400 shoots.  Rooted plantlets were successfully transferred to the field, after acclimation in the net house.   Key words: Baccopa monnieri, Cyanobacterial extract, Regeneration, Acclimation   D.O.I. 10.3329/ptcb.v20i2.6917   Plant Tissue Cult. & Biotech. 20(2): 225-231, 2010 (December)

Effect of Temperature on the Growth Response of Salmonella Enteritidis Inoculated onto the Vitelline Membranes of Fresh Eggs

2003 ◽  
Vol 66 (8) ◽  
pp. 1368-1373 ◽  
Author(s):  
G. J. FLEISCHMAN ◽  
C. L. NAPIER ◽  
D. STEWART ◽  
S. A. PALUMBO
Keyword(s):  
Salmonella Enteritidis ◽  
Growth Response ◽  
Effect Of Temperature ◽  
Vitelline Membrane ◽  
Growth Responses ◽  
In Ovo ◽  
Maximum Increase ◽  
Comparison Of The Results ◽  
As If

The growth response of Salmonella Enteritidis (SE) on the vitelline membrane in vitro was studied with the use of a special tube devised specifically for the inoculation of SE onto the vitelline membrane and for the sampling of the yolk near the inoculation site. This latter ability allowed the detection of the movement of SE into the yolk. The growth of SE on the membrane was compared with that of SE inoculated into yolk and albumen in vitro and in ovo in fresh in-shell eggs. The incubation time was 2 days, and the incubation temperatures were 4, 8, 15, 27, and 37°C. Comparison of the results obtained for in vitro growth showed that at 4, 8, and 15°C, SE behaved as if it were in the albumen, with its numbers decreasing over time. At 27 and 37°C, SE grew as if it were in yolk, with a maximum increase of 4.5 log CFU after 2 days at 37°C. In no experiments involving growth on the vitelline membrane did SE appear in the yolk. Comparisons between in vitro and in ovo growth responses of SE in yolk and albumen indicate that SE growth on the membrane parallels that in the in-shell egg.

scholarly journals The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

2020 ◽  
Vol 23 (1) ◽  
pp. 178-190
Author(s):  
Jeillan Hussein ◽  
Diaa ibraheam
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Nutritional Value ◽  
Culture Media ◽  
Shoot Length ◽  
Ms Medium ◽  
Surface Sterilization

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

scholarly journals In Vitro Propagation of Lilium longiflorum Bulbs Using NAA and BAP Plant Growth Regulator Treatment

2020 ◽  
Author(s):  
Ni Wayan Deswiniyanti ◽  
Ni Kadek Dwipayani Lestari
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Lilium Longiflorum ◽  
Herbaceous Plant ◽  
Perennial Herbaceous Plant ◽  
Plant Parts ◽  
Lily Bulb

Lily (Lilium longiflorum) is a perennial herbaceous plant with white trumpet-shaped flowers, fragrant and bulbous. In vitro culture through bulbs is one of way propagation of lily plants, but it requires a long time and only produces limited plants. In vitro propagation is a very promising technique for plant propagation because it can produce a lot of plant seeds in a short time. Bulbs are one of the fastest explants for growing shoots in lilies, but it is not known for certain which cuts of explants from bulb scales are best for multiplying in vitro. This study aims to determine the effect of lily bulb explants and the concentration of NAA and BAP growth regulators on the growth of lily bulb explants. The best results were obtained on the base and middle cuts explant of bulb scales compared to the tip cuts explant ones. The best results of the growing percentage, the number of shoots and the best growing time are shown in the combination treatment of growth regulator 1 mg L−1 NAA and 1 mg L−1 BAP. The optimum results on the number of micro bulbs were found in the treatment of growth regulators 0.5 mg L−1 NAA and 1 mg L−1 BAP. The best results of the average time formed micro bulb was in the treatment of 1 mg L−1 NAA and BAP with middle explant cuts, and treatment concentrations of 0.5 mg L−1 NAA and BAP in the base explant section. The base and middle bulb explants are able to regenerate or grow higher shoots. This is caused by the presence of endogenous natural auxin and the spread of auxin in plant parts not in the same amount. Therefore when added to the exogenous growth regulator such as auxin or cytokines to culture media will further trigger the formation of micro tubers more quickly,. It can increase the concentration of endogenous growth regulators in cells, help growing process and developing tissue.   Keywords: Bulb, lily, micro bulbs, in vitro, shoots

scholarly journals Propagação in vitro de Zingiber spectabile

2017 ◽  
Vol 23 (3) ◽  
pp. 270
Author(s):  
Michele Valquíria Dos Reis ◽  
Fernanda Carlota Nery ◽  
Débora De Oliveira Prudente ◽  
Patricia Duarte de Oliveira Paiva ◽  
Renato Paiva ◽  
...  
Keyword(s):  
Plant Growth ◽  
Activated Charcoal ◽  
In Vitro Propagation ◽  
Culture Media ◽  
In Vitro Germination ◽  
Phase Pattern ◽  
High Germination ◽  
Length Width ◽  
Ornamental Species

Zingiber spectabile is a tropical ornamental species with difficulties to obtain efficient propagation system. Thus, this study aimed to assess the in vitro propagation of Zingiber spectabile. Seed characterization was determined by measuring length, width and thickness, the weight of 1000 seeds and imbibition curve. In vitro germination of seeds was at constant (25 °C) or alternating temperatures (20-30 ºC). For optimization of in vitro multiplication, different concentrations of activated charcoal (0.0, 0.1 and 0.3%) and sucrose (0.0, 0.1, 0.3, 0.5 and 0.7 M) were evaluated. Plantlets were inoculated in flasks with different sealing systems (PVC covers with or without filters at the center) and culture media (MS or WPM). The plants were acclimatized in Plantmax® substrate. Seeds were of 6.06 mm length, 3.22 mm wide and 2.83 mm thick. The weight of 1,000 seeds corresponded to 46.4 g. The seed imbibition curve approaches to a tree phase pattern. Alternating temperatures induced high germination rates (68%). The addition of 0.3% activated charcoal provided higher root growth and plants with smaller number of senescent leaves. The best plant growth was obtained by the use of 0.1 M sucrose. All acclimatized plants survived (100%). The results demonstrate that Z. spectabile respond well to in vitro propagation.

scholarly journals Surface Roughness of Bulk Fill Composite after Simulated Toothbrushing with Different Dentifrices

2020 ◽  
Vol 8 (D) ◽  
pp. 166-172
Author(s):  
Ahmed Mohamed Elmarakby ◽  
Mahmoud Darwish ◽  
Yasser R. Souror ◽  
Ahmed Waly
Keyword(s):  
Surface Roughness ◽  
Chemical Composition ◽  
Analysis Of Variance ◽  
Composite Resin ◽  
In Vitro Study ◽  
Resin Composites ◽  
Composite Surface ◽  
Significance Level ◽  
Composite Resin Restorations

AIM: The aim of the current in vitro study was to evaluate the changes in surface roughness of bulk fill composites after simulated toothbrushing with different dentifrices. MATERIALS AND METHODS: Three types of bulk fill resin composites were used in this study; 27 specimens of each composite resin were randomly divided into three main groups (n = 9). Each main group was further subdivided into three subgroups (n = 3). Each group was subjected to simulated toothbrushing with three different dentifrices. One-way analysis of variance was used to evaluate the effect of brushing using dentifrices on the surface roughness of each type of composite resin, followed by Tukey’s test at a significance level of p ≤ 0.5%. RESULTS: Results revealed that different effects on composite surface roughness were detected after simulating toothbrushing with different dentifrices. Lacalut toothpaste abrades more with Filtek Bulk Fill, Tetric N-Ceram then Bulk Fill SDR. Crest 3D toothpaste abrades more with Tetric N-Ceram, Bulk Fill SDR then Filtek Bulk Fill. BlanX toothpaste abrades more with Tetric N-Ceram, Bulk Fill SDR then Filtek Bulk Fill. CONCLUSION: Chemical composition of both resin composites and dentifrices plays an important role in influencing the degree of surface roughness of bulk fill composite resin restorations.

scholarly journals Challenges and Opportunity of in vitro Propagation of Paulownia tomentosa Steud for Commercial Production in Nepal

2016 ◽  
Vol 4 (2) ◽  
pp. 155-160
Author(s):  
Lila Bahadur Magar ◽  
Nisha Shrestha ◽  
Saraswoti Khadka ◽  
Jay Raj Joshi ◽  
Jibaraj Acharya ◽  
...  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Royalty Rate ◽  
Paulownia Tomentosa ◽  
Shoot Bud ◽  
Plastic Bags

Paulownia tomentosa Steud is a fast growing multipurpose tree. In vitro propagation using nodal explants of Paulownia tomentosa was performed by manipulating amount of cytokinin and auxin in culture media. Shoot bud proliferation from explants were assessed in Murashige and Skoog (MS) medium supplemented with various amounts of hormones such as a) 0.5-2.5 mg/l benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA), b) 0.5-2.5 mg/l BAP and 0.5-2.5 mg/l kinetin (KN) and c) 0.5- 2.5 mg/l BAP and 0.1 mg/l indole-3-acetic acid (IAA). In the present study, we found that hormone combination of BAP and NAA gave optimum growth results. MS medium enriched with 2.0 mg/l BAP and 0.1 mg/l IAA resulted a similar outcome but took 3-4 weeks with respect to the same medium enriched with 1.0 mg/ml BAP and 0.1 mg/ml NAA, which showed response within 2-3 weeks. Shoot length of 2.5-3.5 cm with 3-4 nodes and 8-12 leaves were obtained on MS medium supplemented with 1.0 mg/l BAP and 0.1 mg/l NAA. The acclimatization of explants was done in a polyhouse at 20±5oC for 2-6 weeks. Rooting has been induced in nonsterile sand. Rooted plants were transferred to plastic bags containing mixture of soil, sand and compost in the ratio of 1:1:1.Besides aforementioned issues, there are several other challenges associated with in vitro propagation of P. tomentosa. The plants were established (90%) on MS medium enriched with BAP and NAA and adapted ex vitro with surviving up to 80%. People received an opportunity with this plant because it grows fast and can generate income in 10 years in comparison with others, but at the same time people also have been facing the challenges for plantation of P. tomentosa as government of Nepal does not formulate necessary national policies, legislations and regulatory frameworks in its favor. Thus, system should be developed to set royalty rate of P. tomentosa recognizing its lifetime value.Int J Appl Sci Biotechnol, Vol 4(2): 155-160

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