In Vitro Propagation of Alexandrian Laurel (Danae racemosa L. Moench), a Valuable Ornamental Plant

To overcome the limitations of traditional propagation, this research was initiated to develop an alternative means for efficient production of Alexandrian laurel (Danae racemosa L. Moench). An in vitro propagation protocol has been developed for Danae racemosa L. Moench using seeds as a source of material for culture initiation. Seedlings were produced after seeds were cultured for 3 month on MS (Murashige and Skoog, 1962) medium. Shoot multiplication occurred on MS medium with or without 6-benzylaminopurine (BAP) with 100% multiplication percentage. However, shoot number was significantly increased from an average of 2.8 to more than six with the addition of 5 or 25 μM BAP. Among two indole-3-butyric acid (IBA) treatments tested for rooting of seedlings, incorporation of 5 μM IBA in MS medium significantly increased rooting percentage to 86.4% compared with 71.2% without IBA. The greatest number of roots (three) was produced by 5-minute IBA pulse. However, both IBA treatments significantly reduced root length. The longest root (12.8 mm) was observed on MS medium without any IBA treatment and the shortest (6.1 mm) was produced by IBA pulse. In vitro-propagated plantlets grew well after transfer to a substrate of peat and pine bark (1:1) in the greenhouse. No morphological variation was observed.

Download Full-text

In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

Archives of Biological Sciences ◽

10.2298/abs1101209u ◽

2011 ◽

Vol 63 (1) ◽

pp. 209-215 ◽

Cited By ~ 1

Author(s):

S. Uranbey

Keyword(s):

Callus Induction ◽

High Frequency ◽

Ornamental Plant ◽

Immature Embryos ◽

Induction Medium ◽

Ms Medium ◽

Culture Initiation ◽

Mineral Salts ◽

Font Color

A high frequency of bulblet regeneration was achieved for the endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos of M. azureum were cultured on a callus induction medium consisting of N6 mineral salts and vitamins, 400 gL-1 casein + 40 gL-1 sucrose + 2 mgL-1 L-proline, 2 mgL-1 2,4-D and 2 gL-1 Gelrite. Then the embryogenic callus clusters were transferred to a bulblet induction medium consisting of MS mineral salts and vitamins containing different concentrations and combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 gL-1 sucrose and 7 gL-1 agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved from immature embryos after 5-6 months of culture initiation. Well-developed bulblets were excised and individually rooted on ? strength MS medium supplemented with 1 mgL-1 IBA, 0.5 gL-1activated charcoal, 20 gL-1sucrose and 6 gL-1agar and acclimatized. This article has been retracted. Link to the retraction <a href="http://dx.doi.org/10.2298/ABS150608072E">10.2298/ABS150608072E</a>

Download Full-text

Copper and Zinc Induced Amelioration of In vitro Multiplication of Dendrocalamus strictus (Roxb.) Nees

Indian Journal of Forestry ◽

10.54207/bsmps1000-2017-vv3901 ◽

2017 ◽

Vol 40 (2) ◽

pp. 181-184

Author(s):

Manish Singh ◽

◽

Manpreet Kaur ◽

Meena Bakshi ◽

Satakshi Kapurwan ◽

...

Keyword(s):

In Vitro Propagation ◽

Shoot Length ◽

Ms Medium ◽

Forest Resource ◽

Similar Concentration ◽

Dendrocalamus Strictus ◽

Copper And Zinc ◽

Shoot Number ◽

Induced Amelioration

Bamboos are among most significant plants worldwide and economically important forest resource in many countries of Asia. In vitro propagation is a potential and well known technique for mass multiplication of bamboo. Efforts were made for multiplication of Dendrocalamus strictus through aseptic nodal explants and its acceleration through heavy metal infusion. A rapid in vitro propagation protocol was established. Maximum germination with 100% survival rate was recorded in MS media supplemented with 1.0 mg/l BAP in combination with TDZ (0.25 mg/l) with a maximum shoot number of 9.36±0.67 and shoot length of 6.85 ± 0.95. The effects of copper and zinc were assessed on morphogenesis. The specific concentration of copper and zinc in MS medium were doubled (2x), quintuple pentadrupled (5x) and decupled (10x). The results revealed that maximum mean shoot number and mean shoot length was observed in quintuple pentadrupled zinc i.e. 6.80 ± 0.74 and 6.60 ± 0.80 cm respectively, however as concerned to copper, maximum shoot number (5.56 ±0.92) and shoot length (5.87 ± 0.78) were observed at similar concentration. Although copper and zinc are toxic for plants at higher concentration, but a good in vitro propagation response was observed in quintuple pentadrupled concentration.

Download Full-text

In vitro propagation of popular banana cultivar (Musa spp. Cv. Patakpura)

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v44i4.45699 ◽

2020 ◽

Vol 44 (4) ◽

pp. 641-648

Author(s):

Bandita Deo ◽

Bikram Keshari ◽

Bikram Pradhan

Keyword(s):

Acetic Acid ◽

In Vitro Propagation ◽

Indole Acetic Acid ◽

Shoot Multiplication ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Musa Sp ◽

Initial Culture ◽

Banana Cultivar

The present experiment was conducted to optimize protocols for in vitro propagation of banana (Musa sp.) cv. ‘Patakpura’ (AAB), supplemented with different growth regulators. Shoot tips obtained from sword suckers were cultured aseptically on MS medium supplemented with different concentrations of cytokinins like 6-Benzylaminopurine (BAP) and Kinetin (KN) for multiplication of shootsand auxins such as indole acetic acid (IAA) and naphthalene acetic acid (NAA) for induction of roots. The best result from the initial culture was obtained from MS medium supplimented with 4 mg/l BAP + 0.5 mg/l IAA. The highest shoot fresh weight, shoot length and number of shoots per explant were recorded from MS medium supplemented with 4 mg/l BAP + 0.5 mg/l IAA + 0.25 mg/l NAA. Therefore, the MS medium supplemented with 4 mg/l BAP + 0.5 mg/l IAA + 0.25 mg/l NAA was found to be most effective and productive combination for shoot multiplication and proliferation of the culture in vitro. IAA at a concentration of 1 mg/l was found to be most suitable for rooting of the shoots. Bangladesh J. Agril. Res. 44(4): 641-648, December 2019

Download Full-text

In vitro propagation of Gentiana dinarica Beck.

Open Life Sciences ◽

10.2478/s11535-012-0059-7 ◽

2012 ◽

Vol 7 (4) ◽

pp. 690-697 ◽

Cited By ~ 4

Author(s):

Branka Vinterhalter ◽

Dijana Milošević ◽

Teodora Janković ◽

Jelena Milojević ◽

Dragan Vinterhalter

Keyword(s):

Plant Growth Regulator ◽

In Vitro Propagation ◽

Shoot Multiplication ◽

Ms Medium ◽

Root Initiation ◽

Free Medium ◽

Rare And Endangered Species ◽

Rare And Endangered ◽

Gentiana Dinarica

AbstractGentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5–1.0 mg l−1 IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.

Download Full-text

OPTIMASI NAA DAN BAP TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TUNAS MIKRO TANAMAN KANTONG SEMAR (Nepenthes mirabilis) SECARA IN VITRO

JURNAL AGROTEKNOLOGI ◽

10.24014/ja.v5i2.1352 ◽

2015 ◽

Vol 5 (2) ◽

pp. 29

Author(s):

ROSMAINA ROSMAINA ◽

DINNI ARYANI

Keyword(s):

In Vitro Culture ◽

Research Design ◽

In Vitro Propagation ◽

Shoot Multiplication ◽

Alternative Method ◽

Shoot Number ◽

Root Explant ◽

Induction Stage

Conventional propagation of Nepenthes was difficult to do. To overcome the problems were required alternative method such as in vitro propagation. The objective of this research was to obtain the best treatment of BAP + NAA on shoot multiplication of Nepenthes through in vitro culture. The research design used Randomized Completely Design consist of seven treatments, e.g. 1) ½ MS0 (control); 2) ½ MS + 1 ppm BAP + 0.5 ppm NAA; 3) ½ MS + 1 ppm BAP + 1 ppm NAA; 4) ½ MS + 1.5 ppm BAP + 0.5 ppm NAA; 5) ½ MS + 1.5 ppm BAP + 1 ppm NAA; 6) ½ MS + 2 ppm BAP + 0.5 ppm NAA dan 7) ½ MS + 2 ppm BAP + 1 ppm NAA. The parameter observed were number of shoot, number of nodul, number of leafs, number of pitcher and number of root. The result of this research showed that treatment of ½ MS + 1 ppm BAP + 1 ppm NAA is the best treatment compared to others. At induction stage, this treatment can produce the number of shoot, number of nodul, and number of root were 1.6 shoots/explant, 10.8 nodul/explant and 3.6 root/explant, respectively. At subculture, this treatment can produce the number of shoot, number of leafs, and number of pitcher were 5.8 shoots/explant, 12.4 leafs/explant and 5.2 pitcher/explant, respectively.

Download Full-text

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

HortScience ◽

10.21273/hortsci13996-19 ◽

2019 ◽

Vol 54 (9) ◽

pp. 1558-1564 ◽

Cited By ~ 3

Author(s):

Georgia Vlachou ◽

Maria Papafotiou ◽

Konstantinos F. Bertsouklis

Keyword(s):

Shoot Multiplication ◽

Adult Plant ◽

Naphthaleneacetic Acid ◽

Similar Response ◽

Ms Medium ◽

Rooting Medium ◽

Shoot Number ◽

Half Strength ◽

Mediterranean Plant

Seed ecophysiology and micropropagation of Clinopodium nepeta, an aromatic Mediterranean plant with pharmaceutical and horticultural uses was investigated. The optimum germination temperature of seeds stored at room temperature for 0, 6, or 12 months was 15 to 20 °C (100% germination completed in 10 to14 days) and cardinal temperatures were defined at 10 and 30 °C (80% to 82% and 62% to 76% germination, respectively). Six or 12 months of storage did not seem to affect germination, although 12-month-old seeds germinated at higher percentage and completed germination earlier at 15 °C than at 20 °C. Concerning micropropagation, shoot multiplication at subcultures of both adult plant- and seedling-origin nodal explants was tested on Murashige and Skoog (MS) medium supplemented with various cytokinin types, i.e., zeatin (ZEA), 6-benzyladenine (BA), kinetin (KIN), and 6-γ-γ-(dimethylallylamino)-purine (2IP), at various concentrations from 0.0 to 8.0 mg·L−1. Both explant types presented a rather similar response during in vitro culture. Increasing concentration of all cytokinin types resulted in an increase in shoot number per responding explant (1.1–5.3) and in most cases a decrease in shoot length (0.6–3.4 cm). Increasing cytokinin concentration induced hyperhydricity to a number of shoots (0.1–6.5) per explant, mostly when ZEA and BA were used. Supplementing the MS medium with 8.0 mg·L−1 BA combined with 0.1 mg·L−1 1-naphthaleneacetic acid (NAA) led to almost elimination of hyperhydricity and very satisfactory shoot production (80%/88% explant response and 6.5/7.5 shoot number per responding explant for seedling- / adult-origin explants, respectively). Alternatively, increasing the agar concentration to 12.0 g·L−1 and supplementing the medium with 8.0 mg·L−1 BA only, resulted in the same effect on eliminating hyperhydricity, such as the addition of NAA, and in the best shoot multiplication response achieved in this study (100% explant response, 9.4/9.9 shoots per explant for seedling-/adult-origin explants, respectively). Microshoots rooted abundantly (92% to 100%) on half-strength MS medium, either Hf or supplemented with 0.5 mg·L−1 to 4.0 mg·L−1 indole-3-butyric acid (IBA). The addition of IBA to the rooting medium, regardless of its concentration, affected only the root length by increasing it 2- to 3-fold. Microshoot clusters produced on multiplication media rooted at 96% when cultured on Hf half-strength MS medium. Rooted microshoots and shoot clusters survived at 80% to 100%, respectively, after ex vitro acclimatization in peat:perlite 1:1 (v/v).

Download Full-text

EFFECT OF SUCROSE AND MEDIA STRENGTH ON IN VITRO MULTIPLICATION IN SWERTIA CHIRATA BUCH.-HAM EX WALL: AN ENDANGERED MEDICINAL HERB

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i11.28135 ◽

2018 ◽

Vol 10 (11) ◽

pp. 40

Author(s):

Manu Pant ◽

Prabha Bisht ◽

Manju P Gusain

Keyword(s):

Shoot Multiplication ◽

Axillary Shoot ◽

Ms Medium ◽

Carbohydrate Source ◽

Windows Nt ◽

Swertia Chirata ◽

Shoot Number ◽

Endangered Medicinal Herb ◽

Experimental Findings

Objective: The present study was performed to investigate the role of varying concentrations of carbohydrate source and strengths of nutrient medium in growth and development of in vitro shoots of Swertia chirata-an endangered medicinal plant.Methods: MS medium supplemented with 6-Benzylaminopurine (4.44 µM), Indole-3 acetic acid (2.85 µM) and Adenine sulphate (271.45 µM) was used to test the efficiency of of sucrose at concentrations of 1-5% and of media strength varying from full to one-fourth. The data were analysed using analysis of variance (ANOVA) of Completely Randomized Design (CRD) in GenStat 5 Edition 3.2 for PC/Windows NT (Copyright 1995, Lawes Agricultural Trust (Rothamsted Experimental Station))Results: Observations on axillary shoot multiplication indicated that sucrose at a concentration of 3% and MS medium in its full strength proved to be most optimal for in vitro culture multiplication. On this medium combination mean number of 11.80 shoots (after 4 w) and 18.50 shoots (after 8 w) could be obtained On sucrose free medium the shoots exhibited necrosis while at lower concentrations of 1-2% sucrose, the shoots developed were thin and unsuitable for further growth in vitro. At higher levels of sucrose in the medium, the shoots became thick and stunted. Similarly, reduction in medium strength resulted in a decline in shoot number and shoot length to an average of 6.50 shoots (1.33 cm mean length) on half strength medium and 5.60 shoots (0.88 cm mean length) on one-fourth strength; as observed after 4 w.Conclusion: The experimental findings suggest that any decline from the standard had a significant effect on the number, size and overall health of shoots developed in vitro. The conditions so standardized augment the production of healthy shoots that shall aid in subsequent rooting and survival after transplantation of tissue-culture raised plantlets.

Download Full-text

In Vitro Regeneration of Grass Pea (Lathyrus sativus L.) from Cotyledonary Node Explants

Journal of Applied Biotechnology ◽

10.5296/jab.v5i2.9121 ◽

2017 ◽

Vol 5 (2) ◽

pp. 57 ◽

Cited By ~ 1

Author(s):

Diriba Tesfaye ◽

Kassahun Bantte ◽

Tewodros Tadesse

Keyword(s):

In Vitro Regeneration ◽

Shoot Multiplication ◽

Full Potential ◽

Ms Medium ◽

Regeneration Capacity ◽

Grass Pea ◽

In Vitro Techniques ◽

Shoot Initiation ◽

Shoot Number

Full potential of grass pea has not been utilized because of the presence of the neurotoxin amino acid β-N-oxalyl-L-αβ -diaminopropionic acid (ODAP/BOAA). Conventional breeding and other approaches have not been successful in reducing the toxin. Integration of in vitro techniques can contribute significantly to meet the challenge. Therefore, this study was carried out to evaluate the in vitro regeneration capacity of grass pea genotypes. Shoot initiation, multiplication and rooting of IVAT-LS-690 were conducted using completely randomized design with five replications. Genotypes were treated with BAP and NAA for shoot initiation while BAP and Kn Combination were used for multiplication. Different concentrations of IBA and IAA were used for rooting. Shoot proliferation percentage was the highest (100%) for IVAT-LS-690,on Murashige and Skoog (MS) medium augmented with 2.0 mg/l BAP +0.1 mg/lNAA.For in vitro shoot multiplication, best results were obtained on concentrations of 3mg/l BAP+1mg/l Kn with maximum shoot number per explants (11.5). High number of roots per shoot (6) and percent of rooted shoot (86.66%) were obtained from ½ MS medium supplemented with 0.5 mg/l IBA. This study inferred that both genotype and BAP levels play a crucial role for shoot regeneration capacity and the optimum hormonal combination for grass pea is genotype specific.

Download Full-text

In vitro propagation of Gerbera (Gerbera jamesonii Bolus)

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2013.7.1.245 ◽

2013 ◽

Vol 7 (1) ◽

pp. 50-58

Author(s):

Sattar Abdullah Shlahi ◽

Duha Mysire Majeed ◽

Salah Mohammed Hasan

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

In Vitro Propagation ◽

Shoot Multiplication ◽

Shoot Formation ◽

Ms Medium ◽

Gerbera Jamesonii

Gerbera plant Gerbera jamesonii is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins kintin (Kin) and benzel adinine (BA), auxin indolacitic acid (IAA). Results revealed that the capitulum showed better response to shoot formation 64.13% whereas the scape did not show response. Yellow flowers showed higher response in shoot formation 37.5% than other strains. growth regulators combination BA and IAA (3.0 + 0.1) mg/L respectively showed better response for shoot multiplication. Auxin IBA (0.5) mg/ L gave better rooting percentage 60% than other auxins IAA and NAA all concentrations. The acclimatization of the gerbera was 78.59%.

Download Full-text

In vitro propagation of some tomato (Lycopersicon esculentum Mill.) hybrids

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2007.1.1.14 ◽

2007 ◽

Vol 1 (1) ◽

pp. 52-60

Author(s):

Kadhum Muhammed Ibrahem ◽

Hamza Musa Kadhum ◽

Ali Abadi Manea

Keyword(s):

Acetic Acid ◽

Light Intensity ◽

Lycopersicon Esculentum ◽

In Vitro Propagation ◽

Indole Acetic Acid ◽

Shoot Multiplication ◽

Ms Medium ◽

Benzyl Adenine ◽

Surface Sterilization

Seeds of four tomato hybrids Ginan, Nirtia, Speedy and Rola were cultured on aseptic germination medium after surface sterilization with NaOCl. Shoot tips 1cm length were dissected and grown on MS medium supplemented with Benzyl adenine (BA) and Indole acetic acid (IAA) for multiplication. Callus was induced on hypocotyls cultured on MS medium supplemented with BA under light intensity of 1000 lux and 25 0C. Results showed that NaOCl at 4% completely disinfected seeds and resulted in pathogen free seedlings. Results also showed that a concentration of 4 mg/l of BA was superior in increasing callus fresh and dry weights. Shoot multiplication was achieved on the same medium supplemented with 2 and 0.8 mg/l of BA and IAA respectively.

Download Full-text