Ex vitro vegetative propagation technique for sustainable utilization of Hypoxis hemerocallidea corms

2021 ◽  
Vol 139 ◽  
pp. 294-299
Author(s):  
Motiki M. Mofokeng ◽  
Hintsa T. Araya ◽  
S.O. Amoo ◽  
C.P. du Plooy ◽  
P.W. Mashela
Author(s):  
Rosanna Ginocchio ◽  
Luz María de la Fuente ◽  
Fabiola Orrego ◽  
María José Díaz ◽  
Javiera Báez ◽  
...  

2019 ◽  
Vol 41 (5) ◽  
Author(s):  
Adelaide de Fátima Santana da Costa ◽  
Emanuel Felipe Medeiros Abreu ◽  
Edilson Romais Schmildt ◽  
Aureliano Nogueira da Costa ◽  
Omar Schmildt

Abstract Papaya (Carica papaya L.) is a fruit tree of great economic and food interest worldwide. Its propagation can occur both sexually and asexually. Traditionally, it is propagated by seeds; however, vegetative propagation has been often used with the use of in vitro and ex vitro culture techniques. In vegetative propagation, the cutting method has been extensively explored. Research has also been developed using grafting. In recent years, papaya cultivation has also become a model for other fruit plants in the use of biotechnological techniques aiming applications ranging from germplasm conservation to genetic transformation of plants. Since the beginning of the 1970s, the culture of papaya tree tissues has been intensely studied, allowing greater control of in vitro development, especially for micropropagation, organogenesis and somatic embryogenesis techniques.


2021 ◽  
Vol 3 (1) ◽  
pp. 129-131
Author(s):  
Muhammad Fuad Yahya ◽  
Nor Hasnida Hassan ◽  
Nazirah Abdullah ◽  
Siti Suhaila Abd. Rahman ◽  
Haliza Ismail ◽  
...  

Eurycoma longifolia is one of the famous herbal plants with great medicinal benefits. The plant which also known as Tongkat Ali is well-known for treating erectile dysfunction and as energy booster. Tongkat Ali plantlets were produced using in vitro propagation technique to cater the demand for planting material. The development of successful acclimatization technique is prerequisite for in vitro propagation method. Acclimatization is a step to prepare the plantlets to survive and grow in different environment compare to laboratory. In this study, an attempt was made to identify the best potting media to acclimatize Tongkat Ali using glass chamber. Rooted plantlets about 2-4 cm height were used and subsequently transferred to different potting media for acclimatization i.e jiffy 7, sand, baked soil, mixture of cocoa peat and sand, sand and top soil and lastly cocoa peat and baked soil. Plantlets grown in jiffy 7 showed 100% survival followed by the mixture of cocoa peat and baked soil with 94.45% of survival. The acclimatized plantlets were transplanted into polybag and maintained in the shadehouse condition.


2018 ◽  
Vol 3 (02) ◽  
pp. 132-136
Author(s):  
S. K. Kalita ◽  
A. K. Singh ◽  
V. K. Mishra ◽  
Wajid Hasan

Eclipta prostata L. commonly known as Bhringaraj is one of the most valuable and important medicinal herb. The whole plants are used in medicinal and cosmetic industries. But still it is considered as a wild weed due to lack of propagation technique. Vegetative propagation plays an important role for its commercial cultivation. The experiment on “Rooting behavior of stem cuttings of Eclipta prostata L. with varying concentrations of different plant hormones” was laid out under CRD design with 13 treatments and 3 replications at demonstration unit of KVK Tirap, Deomali, Arunachal Pradesh. From this experiment it could be concluded that IBA treated cuttings irrespective of concentration was the best for rooting in Eclipta prostata cuttings under climatic condition of Tirap . The highest percentage of rooting, number of roots, survivability percentage and minimum days to rooting were recorded in IBA 400 ppm, IBA 300 ppm, IBA 100 ppm and IBA 400 ppm treated cuttings respectively.


2010 ◽  
Vol 10 (1) ◽  
pp. 54-64
Author(s):  
R. K. Patel ◽  
K. D. Babu ◽  
Akath Singh ◽  
D. S. Yadav ◽  
L. C. De

2016 ◽  
Vol 10 (2) ◽  
pp. 183
Author(s):  
Djadja Siti Hazar Hoesen

Buds, planlets and bulblets formation from excised bulbscales was the preferredmethod for vegetative propagation of Lilium sp (Liliaceae). The ex vitro techniqueswith Gibberellic acid (GA3) pretreatment was induced buds formation on scalescutting which planted on sterilized sand media. Buds rised from basal scales 7days after planted. However scales untreated GA3 obtained in 35-42 after planted.In vitro methods to promote buds initiated from bulbscales explants, was inducedon media MS (Murashige and Skoog) supplemented with GA3 1 mg/l. Media forinduced buds formation, MS contained Benzyl adenine (BA) 1 mg/l and 2 mg/lincreased multiple shoots formation significantly compared cultured on mediawithout BA. Roots growth improved on media contained NAA, but the highestplanlets achieved on cultured MS media without BA. Bulblets formation obtainedon media contained higher concentration of BA (5 mg/l).


2018 ◽  
Vol 19 (6) ◽  
pp. 2353-2358
Author(s):  
HENTI HENDALASTUTI RACHMAT ◽  
ATOK SUBIAKTO ◽  
ARIDA SUSILOWATI

Rachmat HH, Subiakto A, Susilowati A. 2018. Mass vegetative propagation of rare and endangered tree species of Indonesia by shoot cuttings by KOFFCO method and effect of container type on nursery storage of rooted cuttings. Biodiversitas 19: 2353-2358. The Forestry Research and Development Agency (FORDA) and The Advance Research of Komatsu Ltd have developed a mass propagation technique using shoot cuttings, namely KOFFCO system (Komatsu-FORDA Fog Cooling System). KOFFCO system controls environmental factors (temperature, humidity, and light intensity) to produce high percentage of rooted cuttings. The seed production of dipterocarp species is unreliable and unpredictable. Thus, vegetative propagation is an alternative means for producing planting materials of dipterocarp species. An attempt was made to produce rooted cuttings of rare and endangered dipterocarp and non-dipterocarp species of the Indonesian native trees, for further use in both reforestation and plantation programs. In fact, this study attempts to answer two specific research questions: (i) What is the potential of KOFFCO system as a vegetative mass propagation technique for Indonesian dipterocarps and non-dipterocarps? (ii) What is the effect of container types on long-term post-nursery storage of dipterocarp planting stock? To answer the first question stated above, vegetative propagation through shoot cuttings of 24 species belonging to 7 genera of dipterocarps and 13 species belonging to 12 genera of non-dipterocarps was attempted (>30.000 cuttings, in total), using KOFFCO system. The results showed that KOFFCO is an efficient innovative technology for the successful mass propagation from cuttings. 100% root formation obtained for Nauclea orientalis, Anotephalus macrophyllus and Ficus sp. To find the answer for the second question, storage experiments and growth observations of the planting stock of selected dipterocarp species was performed, using three different types of containers. Rooted cuttings of six species of dipterocarps with same germination date were tested in each type of the container. The result showed that medium sized square and medium sized round containers produced an average height increment of 19.92 cm and 21.58 cm, respectively, in a year, while small sized square containers produced only 13.94 cm height increment. Both square and round shaped containers are appropriate for short term storage of planting stock, while small containers are more appropriate for storing planting stocks or seedlings for a longer period.


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