TROSY in NMR studies of the structure and function of large biological macromolecules

2003 ◽  
Vol 13 (5) ◽  
pp. 570-580 ◽  
Author(s):  
C FERNANDEZ
Keyword(s):  
Structure And Function ◽  
Biological Macromolecules ◽  
Nmr Studies ◽  
And Function

Depletion and Reconstitution of Active E. Coli Ribosomes

1975 ◽  
Vol 33 ◽  
pp. 640-641
Author(s):  
M. Boublik ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Protein Biosynthesis ◽  
Large Subunit ◽  
High Resolution Electron Microscopy ◽  
Small Subunit ◽  
Structure And Function ◽  
Biologically Active ◽  
Biological Macromolecules ◽  
E Coli ◽  
Resolution Electron ◽  
And Function

Correlations between structure and function of biological macromolecules have been studied intensively for many years, mostly by indirect methods. High resolution electron microscopy is a unique tool which can provide such information directly by comparing the conformation of biopolymers in their biologically active and inactive state. We have correlated the structure and function of ribosomes, ribonucleoprotein particles which are the site of protein biosynthesis. 70S E. coli ribosomes, used in this experiment, are composed of two subunits - large (50S) and small (30S). The large subunit consists of 34 proteins and two different ribonucleic acid molecules. The small subunit contains 21 proteins and one RNA molecule. All proteins (with the exception of L7 and L12) are present in one copy per ribosome.This study deals with the changes in the fine structure of E. coli ribosomes depleted of proteins L7 and L12. These proteins are unique in many aspects.

scholarly journals Thomas Arthur Steitz. 23 August 1940—9 October 2018

2021 ◽  
Author(s):  
V. Ramakrishnan ◽  
Richard Henderson
Keyword(s):  
Nobel Prize ◽  
Genetic Information ◽  
Metabolic Enzymes ◽  
Structure And Function ◽  
Structural Basis ◽  
Biological Macromolecules ◽  
Research Career ◽  
And Function

Thomas A. Steitz was among the foremost of the generation that was responsible for an explosion in our understanding of the structure and function of biological macromolecules. His research career was one of sustained excellence over six decades, and spanned the range from determining the structures of important metabolic enzymes to understanding the structural basis of how genetic information residing in our DNA is used to make the proteins they encode. This latter effort culminated in the structure of the ribosome, for which he shared the Nobel Prize in Chemistry in 2009.

scholarly journals Notes On Synthesis Of Perdeutero-5-13C,5,5,5-Trifluoroisoleucine VI

2017 ◽  
Author(s):  
David Naugler ◽  
Robert Scott Prosser
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Structure And Function ◽  
Methyl Groups ◽  
Nmr Studies ◽  
Branched Chain Amino Acid ◽  
Acid Methyl ◽  
Deuterated Proteins ◽  
Branched Chain ◽  
And Function

The 13CF3 group is a promising label for heteronuclear (19F,13C) NMR studies of proteins. Desirable locations for this NMR spin label include the branched chain amino acid methyl groups. It is known that replacement of CH3 by CF3 at such locations preserves protein structure and function and enhances stability. In particular, 13CF3 may be introduced at the δ position of isoleucine and incorporated biosynthetically in highly deuterated proteins. This paper reports our work in synthesis and purification of 5,5,5-trifluoroisoleucine, its perdeutero and 5-13C versions and of 2-13C-trifluoroacetate and its utility as a precursor for introduction of the 13CF3 group into proteins.

scholarly journals A Simple Method for Imaging DNA using SEM

2008 ◽  
Vol 16 (2) ◽  
pp. 38-39
Author(s):  
N. Chatterjee ◽  
K. Andresen ◽  
M. Thomas ◽  
L. Pollack ◽  
E. Kirkland
Keyword(s):  
Molecular Structure ◽  
Electron Microscope ◽  
Structure And Function ◽  
Biological Macromolecules ◽  
Common Theme ◽  
Simple Method ◽  
Transmission Electron ◽  
And Function ◽  
Molecular Structure And Function ◽  
Scanning Electron

The intricate relationship between molecular structure and function is a common theme in molecular biology. Visualizing the structure of biological macromolecules through imaging is therefore useful in understanding their varied biological roles. The process is often complex; imaging in a high voltage Transmission Electron Microscope (TEM) involves extensive staining and freezing. The aim of this experiment was to image DNA easily in a close to natural environment in a simple microscope. Samples were imaged using a Leo (Zeiss) 1550 Scanning Electron Microscope (SEM) with a Schottky field emitter in the 15-30kV range to reduce radiation damage. After imaging off-the-shelf DNA, two more DNA samples were dialyzed with RbCl and NaCl and imaged to elucidate what made the DNA visible.

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