Indirect regeneration from in vitro grown leaves of three pear cultivars and determination of ploidy level in regenerated shoots by flow cytometry

2013 ◽  
Vol 164 ◽  
pp. 455-460 ◽  
Author(s):  
Somaye Javadi ◽  
Maryam Jafarkhani Kermani ◽  
Saeed Irian ◽  
Ahmad Majd
Keyword(s):  
Flow Cytometry ◽  
Ploidy Level ◽  
Indirect Regeneration

Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum: in vivo and in vitro studies

1990 ◽  
Vol 36 (9) ◽  
pp. 1638-1641 ◽  
Author(s):  
S C Kazmierczak ◽  
W J Castellani ◽  
F Van Lente ◽  
E D Hodges ◽  
B Udis
Keyword(s):  
Flow Cytometry ◽  
Lactate Dehydrogenase ◽  
Cell Types ◽  
In Vitro Studies ◽  
Hemolytic Disease ◽  
Dehydrogenase Isoenzyme ◽  
Disease Analysis

Abstract We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenzyme distribution between these two cell types. The ability of hemolysis to cause a "flipped" LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.

IN VITRO DETERMINATION OF THE APOPTOTIC EFFECT OF HEPARIN ON LYMPHOBLASTS USING DNA ANALYSIS AND MEASUREMENTS OF Fas AND Bcl-2 PROTEINS BY FLOW CYTOMETRY

2004 ◽  
Vol 21 (5) ◽  
pp. 383-391 ◽  
Author(s):  
Erol Erduran ◽  
Yavuz Tekelioglu ◽  
Yusuf Gedik ◽  
İsmail Bektaş ◽  
Sadan Hacisalihoglu
Keyword(s):  
Flow Cytometry ◽  
Dna Analysis ◽  
Apoptotic Effect

scholarly journals Polyploidy induction in Phlox paniculata L. under in vitro conditions

2010 ◽  
Vol 58 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Pavel Matiska ◽  
Hana Vejsadová
Keyword(s):  
Flow Cytometry ◽  
Ploidy Level ◽  
Chemical Mutagen ◽  
Nutrient Media ◽  
Phlox Paniculata ◽  
Diploid Cultivar ◽  
Infiltration Method

The objective of this work was to find an effective method of polyploidy induction using chemomutagens, colchicine and oryzalin, in diploid cultivar of Phlox paniculata ‘Fujiyama’ (syn. Mt. Fuji, Fuji). Ploidy level was determined by the flow cytometry method (FCM). Two methods of treating the explants (in vitro regenerated shoots) were tested; chemomutagen infiltration from nutrient media (“the infiltration method”) and dipping of the explants in a chemical mutagen solution (“the dip method”). The highest values of tetraploid (5%), mixoploid (1.67%) frequency and polyploidization efficiency (1.25) were found in explants treated with 0.2% colchicine for 24 h in the dip method. Concentrations of 10 µM oryzalin and 0.2% colchicine for 14 d were the most effective for obtaining tetraploids in the infiltration method. The results will be exploited to other P. paniculata cultivars for breeding of this species.

Use of flow cytometry for rapid determination of ploidy level in the genus Actinidia

1994 ◽  
Vol 57 (4) ◽  
pp. 303-313 ◽  
Author(s):  
Fréderique Ollitrault-Sammarcelli ◽  
J.M. Legave ◽  
Nicole Michaux-Ferriere ◽  
Anne Marie Hirsch
Keyword(s):  
Flow Cytometry ◽  
Ploidy Level ◽  
Rapid Determination

scholarly journals Determination of Ploidy Level and Nuclear DNA Content in the Droseraceae by Flow Cytometry

CYTOLOGIA ◽  
2017 ◽  
Vol 82 (3) ◽  
pp. 321-327 ◽  
Author(s):  
Yoshikazu Hoshi ◽  
Masako Azumatani ◽  
Chika Suyama ◽  
Lubomίr Adamec
Keyword(s):  
Flow Cytometry ◽  
Ploidy Level ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

scholarly journals (379) Ploidy Determinationin Vitis rotundifolia using Flow Cytometry

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1068C-1068
Author(s):  
Xia Xu ◽  
Jiang Lu ◽  
Zhongbo Ren
Keyword(s):  
Flow Cytometry ◽  
Ploidy Level ◽  
Nuclear Dna ◽  
Radiation Detection ◽  
Nuclear Dna Content ◽  
Genetic Studies ◽  
Ploidy Levels ◽  
In Vitro Techniques ◽  
Vitis Rotundifolia

Ploidy level in grapevines varies, especially since in vitro techniques are employed in the breeding process and after plants are treated with either chemicals or radiation. Detection of ploidy level in grapevines by microscopic chromosome counting is complicated by their high number and the small size of chromosomes. Flow cytometry provides an accurate and rapid method in determining the ploidy level in plant tissue by measuring the nuclear DNA content in living cells and thus is a very useful tool in plant breeding or genetic studies. The objective of this research was to analyze the ploidy level of a selected group of muscadine vines that were different from normal diploid vines in morphology. These grapes were derived from either chemical treatment of known varieties or from controlled/open pollinations. Among the 26 grapevines investigated, 8 were found to be diploids, 11 were tetraploids, and 7 were chimeric aneuploids. Results of this study indicate that flow cytometry is a quick, reliable tool for determining ploidy levels of grapevines.

scholarly journals Determination of ploidy level and nuclear DNA content in blueberry by flow cytometry

1993 ◽  
Vol 86 (8) ◽  
pp. 1001-1006 ◽  
Author(s):  
D. E. Costich ◽  
R. Ortiz ◽  
T. R. Meagher ◽  
L. P. Bruederle ◽  
N. Vorsa
Keyword(s):  
Flow Cytometry ◽  
Ploidy Level ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content
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