Mapping of powdery mildew resistance genes in melon ( Cucumis melo L.) by bulked segregant analysis

AbstractMelon (Cucumis melo L.) is wildly planted in the world and China is a major producer of muskmelon. Powdery mildew is one of the most common fungal diseases in the world and this disease frequently affects melon (Cucumis melo L.) and due to the reduction of melon yield. In this study, one material GanTianmi with thin peel and another material XueLianHua with thick peel were selected. After inoculating the powdery mildew, both materials were used to do the RNA-Seq. In total two RNA-seq libraries were constructed and sequenced separately. The reads per kilobase per Million mapped reads (RPKM) values of all the genes in the two materials were calculated and there were 13828 genes were expressed in the material G and 13944 genes were expressed in the material S (RPKM>1). The differentially expression gene (DEG) analysis result suggested that total 769 the DEGs between the two materials were identified. All the DEGs were annotated with several database and the transcript factors (TFs) that related to disease resistance such as MYB, ERF and WRKY among the DEGs were also identified. This research could not only provide the information about understanding the mechanism of powdery mildew infection but also help researchers breed the varieties with powdery mildew resistance.

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Application of comparative genomics in developing markers tightly linked to the Pm-2F gene for powdery mildew resistance in melon (Cucumis melo L.)

Euphytica ◽

10.1007/s10681-012-0828-4 ◽

2012 ◽

Vol 190 (2) ◽

pp. 157-168 ◽

Cited By ~ 12

Author(s):

Chunqiu Zhang ◽

Yi Ren ◽

Shaogui Guo ◽

Haiying Zhang ◽

Guoyi Gong ◽

...

Keyword(s):

Comparative Genomics ◽

Powdery Mildew ◽

Cucumis Melo ◽

Powdery Mildew Resistance ◽

Mildew Resistance ◽

Cucumis Melo L

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Identification of AFLP markers closely linked to the powdery mildew resistance genes Pm1c and Pm4a in common wheat (Triticum aestivum L.)

Genome ◽

10.1139/g98-129 ◽

1999 ◽

Vol 42 (2) ◽

pp. 322-329 ◽

Cited By ~ 22

Author(s):

Lorenz Hartl ◽

Volker Mohler ◽

Friedrich J Zeller ◽

Sai LK Hsam ◽

Günther Schweizer

Keyword(s):

Triticum Aestivum ◽

Powdery Mildew ◽

Resistance Gene ◽

Resistance Genes ◽

Powdery Mildew Resistance ◽

Bulked Segregant Analysis ◽

Triticum Aestivum L ◽

Aflp Markers ◽

Aflp Analysis ◽

Mildew Resistance

A total of 7654 DNA fragments were screened for linkage to wheat powdery mildew resistance gene Pm1c employing fluorescently based AFLP analysis and phenotypic pools from F3 families. F3 and derived F4 families were used for segregation analysis. Pool screening revealed several cosegregating and tightly linked (0.9 cM) AFLP markers for the Pm1c resistance gene. The previously reported RFLP locus Xwhs178 was integrated into the AFLP map in the vicinity of Pm1c. One AFLP marker, 18M2, was determined to be highly specific for the Pm1c gene in diverse genetic backgrounds. As Pm1c allele confers an effective resistance to powdery mildew, the marker 18M2 provides a valuable tool for enhancing marker assisted selection and pyramiding of powdery mildew resistance genes in wheat.Key words: Triticum aestivum, powdery mildew, disease resistance, AFLP, bulked segregant analysis

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