Genetic linkage map of melon (Cucumis melo L.) and localization of a major QTL for powdery mildew resistance

2010 ◽  
Vol 27 (2) ◽  
pp. 181-192 ◽  
Author(s):  
Fernando J. Yuste-Lisbona ◽  
Carmen Capel ◽  
Emilio Sarria ◽  
Rocío Torreblanca ◽  
María L. Gómez-Guillamón ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Linkage Map ◽  
Cucumis Melo ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L ◽  
Major Qtl

scholarly journals Development of SNP, KASP, and SSR Markers by BSR-Seq Technology for Saturation of Genetic Linkage Map and Efficient Detection of Wheat Powdery Mildew Resistance Gene Pm61

2019 ◽  
Vol 20 (3) ◽  
pp. 750 ◽  
Author(s):  
Jinghuang Hu ◽  
Jingting Li ◽  
Peipei Wu ◽  
Yahui Li ◽  
Dan Qiu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Linkage Map ◽  
Ssr Markers ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Mildew Resistance ◽  
Specific Pcr ◽  
Efficient Detection

The gene Pm61 that confers powdery mildew resistance has been previously identified on chromosome arm 4AL in Chinese wheat landrace Xuxusanyuehuang (XXSYH). To facilitate the use of Pm61 in breeding practices, the bulked segregant analysis-RNA-Seq (BSR-Seq) analysis, in combination with the information on the Chinese Spring reference genome sequence, was performed in the F2:3 mapping population of XXSYH × Zhongzuo 9504. Two single nucleotide polymorphism (SNP), two Kompetitive Allele Specific PCR (KASP), and six simple sequence repeat (SSR) markers, together with previously identified polymorphic markers, saturated the genetic linkage map for Pm61, especially in the proximal side of the target gene that was short of gene-linked markers. In the newly established genetic linkage map, Pm61 was located in a 0.71 cM genetic interval and can be detected in a high throughput scale by the KASP markers Xicsk8 and Xicsk13 or by the standard PCR-based markers Xicscx497 and Xicsx538. The newly saturated genetic linkage map will be useful in molecular marker assisted-selection of Pm61 in breeding for disease resistant cultivar and in its map-based cloning.

Codominant PCR-based markers and candidate genes for powdery mildew resistance in melon (Cucumis melo L.)

2011 ◽  
Vol 122 (4) ◽  
pp. 747-758 ◽  
Author(s):  
Fernando J. Yuste-Lisbona ◽  
Carmen Capel ◽  
María L. Gómez-Guillamón ◽  
Juan Capel ◽  
Ana I. López-Sesé ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Candidate Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

Mapping of powdery mildew resistance genes in melon ( Cucumis melo L.) by bulked segregant analysis

2017 ◽  
Vol 220 ◽  
pp. 160-167 ◽  
Author(s):  
Bing Li ◽  
Yulong Zhao ◽  
Qianglong Zhu ◽  
Zhipeng Zhang ◽  
Chao Fan ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Mildew Resistance ◽  
Cucumis Melo L

scholarly journals Comparative transcriptome analysis of Powdery mildew Resistance between two Melon (Cucumis melo L) with Different Thickness Peel

2019 ◽  
Author(s):  
Cheng Hong ◽  
Kong Wei-ping ◽  
Lü Jun-Feng
Keyword(s):  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Rna Seq ◽  
Mildew Resistance ◽  
The World ◽  
Major Producer ◽  
Cucumis Melo L ◽  
Different Thickness ◽  
Powdery Mildew Infection

AbstractMelon (Cucumis melo L.) is wildly planted in the world and China is a major producer of muskmelon. Powdery mildew is one of the most common fungal diseases in the world and this disease frequently affects melon (Cucumis melo L.) and due to the reduction of melon yield. In this study, one material GanTianmi with thin peel and another material XueLianHua with thick peel were selected. After inoculating the powdery mildew, both materials were used to do the RNA-Seq. In total two RNA-seq libraries were constructed and sequenced separately. The reads per kilobase per Million mapped reads (RPKM) values of all the genes in the two materials were calculated and there were 13828 genes were expressed in the material G and 13944 genes were expressed in the material S (RPKM>1). The differentially expression gene (DEG) analysis result suggested that total 769 the DEGs between the two materials were identified. All the DEGs were annotated with several database and the transcript factors (TFs) that related to disease resistance such as MYB, ERF and WRKY among the DEGs were also identified. This research could not only provide the information about understanding the mechanism of powdery mildew infection but also help researchers breed the varieties with powdery mildew resistance.

Application of comparative genomics in developing markers tightly linked to the Pm-2F gene for powdery mildew resistance in melon (Cucumis melo L.)

Euphytica ◽  
2012 ◽  
Vol 190 (2) ◽  
pp. 157-168 ◽  
Author(s):  
Chunqiu Zhang ◽  
Yi Ren ◽  
Shaogui Guo ◽  
Haiying Zhang ◽  
Guoyi Gong ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

Identification of QTLs for resistance to powdery mildew and SSR markers diagnostic for powdery mildew resistance genes in melon (Cucumis melo L.)

2008 ◽  
Vol 118 (1) ◽  
pp. 165-175 ◽  
Author(s):  
Nobuko Fukino ◽  
Takayoshi Ohara ◽  
Antonio J. Monforte ◽  
Mitsuhiro Sugiyama ◽  
Yoshiteru Sakata ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

CONSTRUCTION OF A GENETIC LINKAGE MAP AND IDENTIFICATION OF A MAJOR QTL CONFERRING RESISTANCE TO POWDERY MILDEW IN THE MELON GENOTYPE TGR-1551

2010 ◽  
pp. 179-186 ◽  
Author(s):  
F.J. Yuste-Lisbona ◽  
C. Capel ◽  
E. Sarria ◽  
M.L. Gómez-Guillamón ◽  
J. Capel ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Major Qtl

scholarly journals Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

BIOEDUSCIENCE ◽  
2020 ◽  
Vol 4 (1) ◽  
pp. 1-10
Author(s):  
Muhammad Alif Ishak ◽  
Budi Setiadi Daryono
Keyword(s):  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Morphological Characters ◽  
Lateral Part ◽  
Tolerance Level ◽  
Melon Seeds ◽  
Cucumis Melo L ◽  
Powdery Mildew Infection ◽  
Level Resistance

Background: A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. Meloni is a superior melon cultivar bred through a crossing between ♀ SL-3 and ♂ PI 371795, resulted by the Laboratory of Genetics and Breeding, Faculty of Biology, UGM. This study aimed to determine the level resistance of Meloni to powdery mildew infection and to identify the powdery mildew species that infected Meloni based on morphological characters. Methods: Meloni seeds were germinated and planted in the greenhouse of PIAT UGM. Powdery mildew spores were inoculated into the leaves after ±2 weeks of age. Leaf infected were scored using the gridline every 3 days for 6 weeks. Scoring results were converted to the diseases index score. Furthermore, powdery mildew species was identified using morphological characters. Results: Meloni had a tolerance level of resistance to the powdery mildew infection. Based on the morphological characters with fibrosin bodies, conidia ovoid-shape and the position of the germ tube in the lateral part of the conidia, powdery mildew that infected Meloni was expected as P. xanthii. Conclusions: Meloni can be expected as an alternative to superior melon seeds resistant to pest and disease infections especially powdery mildew.  

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