Codominant PCR-based markers and candidate genes for powdery mildew resistance in melon (Cucumis melo L.)

2011 ◽  
Vol 122 (4) ◽  
pp. 747-758 ◽  
Author(s):  
Fernando J. Yuste-Lisbona ◽  
Carmen Capel ◽  
María L. Gómez-Guillamón ◽  
Juan Capel ◽  
Ana I. López-Sesé ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Candidate Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

Mapping of powdery mildew resistance genes in melon ( Cucumis melo L.) by bulked segregant analysis

2017 ◽  
Vol 220 ◽  
pp. 160-167 ◽  
Author(s):  
Bing Li ◽  
Yulong Zhao ◽  
Qianglong Zhu ◽  
Zhipeng Zhang ◽  
Chao Fan ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Mildew Resistance ◽  
Cucumis Melo L

Genetic linkage map of melon (Cucumis melo L.) and localization of a major QTL for powdery mildew resistance

2010 ◽  
Vol 27 (2) ◽  
pp. 181-192 ◽  
Author(s):  
Fernando J. Yuste-Lisbona ◽  
Carmen Capel ◽  
Emilio Sarria ◽  
Rocío Torreblanca ◽  
María L. Gómez-Guillamón ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Linkage Map ◽  
Cucumis Melo ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L ◽  
Major Qtl

scholarly journals Comparative transcriptome analysis of Powdery mildew Resistance between two Melon (Cucumis melo L) with Different Thickness Peel

2019 ◽  
Author(s):  
Cheng Hong ◽  
Kong Wei-ping ◽  
Lü Jun-Feng
Keyword(s):  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Rna Seq ◽  
Mildew Resistance ◽  
The World ◽  
Major Producer ◽  
Cucumis Melo L ◽  
Different Thickness ◽  
Powdery Mildew Infection

AbstractMelon (Cucumis melo L.) is wildly planted in the world and China is a major producer of muskmelon. Powdery mildew is one of the most common fungal diseases in the world and this disease frequently affects melon (Cucumis melo L.) and due to the reduction of melon yield. In this study, one material GanTianmi with thin peel and another material XueLianHua with thick peel were selected. After inoculating the powdery mildew, both materials were used to do the RNA-Seq. In total two RNA-seq libraries were constructed and sequenced separately. The reads per kilobase per Million mapped reads (RPKM) values of all the genes in the two materials were calculated and there were 13828 genes were expressed in the material G and 13944 genes were expressed in the material S (RPKM>1). The differentially expression gene (DEG) analysis result suggested that total 769 the DEGs between the two materials were identified. All the DEGs were annotated with several database and the transcript factors (TFs) that related to disease resistance such as MYB, ERF and WRKY among the DEGs were also identified. This research could not only provide the information about understanding the mechanism of powdery mildew infection but also help researchers breed the varieties with powdery mildew resistance.

Application of comparative genomics in developing markers tightly linked to the Pm-2F gene for powdery mildew resistance in melon (Cucumis melo L.)

Euphytica ◽  
2012 ◽  
Vol 190 (2) ◽  
pp. 157-168 ◽  
Author(s):  
Chunqiu Zhang ◽  
Yi Ren ◽  
Shaogui Guo ◽  
Haiying Zhang ◽  
Guoyi Gong ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

Identification of QTLs for resistance to powdery mildew and SSR markers diagnostic for powdery mildew resistance genes in melon (Cucumis melo L.)

2008 ◽  
Vol 118 (1) ◽  
pp. 165-175 ◽  
Author(s):  
Nobuko Fukino ◽  
Takayoshi Ohara ◽  
Antonio J. Monforte ◽  
Mitsuhiro Sugiyama ◽  
Yoshiteru Sakata ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Cucumis Melo L

scholarly journals Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

BIOEDUSCIENCE ◽  
2020 ◽  
Vol 4 (1) ◽  
pp. 1-10
Author(s):  
Muhammad Alif Ishak ◽  
Budi Setiadi Daryono
Keyword(s):  
Powdery Mildew ◽  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Morphological Characters ◽  
Lateral Part ◽  
Tolerance Level ◽  
Melon Seeds ◽  
Cucumis Melo L ◽  
Powdery Mildew Infection ◽  
Level Resistance

Background: A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. Meloni is a superior melon cultivar bred through a crossing between ♀ SL-3 and ♂ PI 371795, resulted by the Laboratory of Genetics and Breeding, Faculty of Biology, UGM. This study aimed to determine the level resistance of Meloni to powdery mildew infection and to identify the powdery mildew species that infected Meloni based on morphological characters. Methods: Meloni seeds were germinated and planted in the greenhouse of PIAT UGM. Powdery mildew spores were inoculated into the leaves after ±2 weeks of age. Leaf infected were scored using the gridline every 3 days for 6 weeks. Scoring results were converted to the diseases index score. Furthermore, powdery mildew species was identified using morphological characters. Results: Meloni had a tolerance level of resistance to the powdery mildew infection. Based on the morphological characters with fibrosin bodies, conidia ovoid-shape and the position of the germ tube in the lateral part of the conidia, powdery mildew that infected Meloni was expected as P. xanthii. Conclusions: Meloni can be expected as an alternative to superior melon seeds resistant to pest and disease infections especially powdery mildew.  

Genetic Mapping of Adult-Plant Resistance Genes to Powdery Mildew in Triticale

2021 ◽  
Author(s):  
Mateusz Maksymilian Dyda ◽  
Mirosław Tyrka ◽  
Gabriela Gołębiowska ◽  
Marcin Rapacz ◽  
Maria Wędzony
Keyword(s):  
Powdery Mildew ◽  
Candidate Genes ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Doubled Haploids ◽  
Rapid Development ◽  
Genetic Maps ◽  
Adult Plant ◽  
Mildew Resistance ◽  
Progress Curve

Abstract Triticale is a cereal of high economic importance, however along with the increase in the area of this cereal, it is more often infected by the fungal pathogen Blumeria graminis, which causes powdery mildew. The rapid development of molecular biology techniques, in particular methods based on molecular markers may be an important tool used in modern plant breeding. Development of genetic maps, location of the QTLs defining the region of the genome associated with resistance and selection of markers linked to particular trait can be used to select resistant genotypes as well as to pyramidize several resistance genes in one variety. In this paper we present a new, high-density genetic map of triticale doubled haploids (DH) population ‘Grenado’ x ‘Zorro’ composed of DArT, silicoDArT and SNP markers. Composite interval mapping method was used to detect eight QTL regions associated with the area under disease progress curve (AUDPC) and 15 regions with the average value of powdery mildew infection (avPM) based on observation conducted in 3-year period in three different locations across the Poland. Two regions on rye chromosome 4R, and single loci on 5R and 6R were reported for the first time as regions associated with powdery mildew resistance. Among all QTLs, 14 candidate genes were identified coded cyclin-dependent kinase, serine/threonine-protein kinase-like protein as well as AMEIOTIC 1 homolog DYAD-like protein, DETOXIFICATION 16-like protein and putative disease resistance protein RGA3. Three of identified candidate genes were found among newly described QTL regions associated with powdery mildew resistance in triticale.

scholarly journals Basal Host Resistance of Barley to Powdery Mildew: Connecting Quantitative Trait Loci and Candidate Genes

2010 ◽  
Vol 23 (1) ◽  
pp. 91-102 ◽  
Author(s):  
Reza Aghnoum ◽  
Thierry C. Marcel ◽  
Annika Johrde ◽  
Nicola Pecchioni ◽  
Patrick Schweizer ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Powdery Mildew ◽  
Candidate Genes ◽  
Quantitative Trait ◽  
Powdery Mildew Resistance ◽  
Adult Plant ◽  
Basal Resistance ◽  
Mildew Resistance ◽  
Trait Loci ◽  
Mapping Populations

The basal resistance of barley to powdery mildew (Blumeria graminis f. sp. hordei) is a quantitatively inherited trait that is based on nonhypersensitive mechanisms of defense. A functional genomic approach indicates that many plant candidate genes are involved in the defense against formation of fungal haustoria. It is not known which of these candidate genes have allelic variation that contributes to the natural variation in powdery mildew resistance, because many of them may be highly conserved within the barley species and may act downstream of the basal resistance reaction. Twenty-two expressed sequence tag or cDNA clone sequences that are likely to play a role in the barley–Blumeria interaction based on transcriptional profiling, gene silencing, or overexpression data, as well as mlo, Ror1, and Ror2, were mapped and considered candidate genes for contribution to basal resistance. We mapped the quantitative trait loci (QTL) for powdery mildew resistance in six mapping populations of barley at seedling and adult plant stages and developed an improved high-density integrated genetic map containing 6,990 markers for comparing QTL and candidate gene positions over mapping populations. We mapped 12 QTL at seedling stage and 13 QTL at adult plant stage, of which four were in common between the two developmental stages. Six of the candidate genes showed coincidence in their map positions with the QTL identified for basal resistance to powdery mildew. This co-localization justifies giving priority to those six candidate genes to validate them as being responsible for the phenotypic effects of the QTL for basal resistance.

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