Role of sucrose phosphate synthase and vacuolar invertase in postharvest sweetening of immature sweetpotato tuberous roots (Ipomoea batatas (L.) Lam cv ‘Xinxiang’)

2021 ◽  
Vol 282 ◽  
pp. 110007
Author(s):  
Lei Ru ◽  
Baowei Chen ◽  
Yongxin Li ◽  
R.B.H. Wills ◽  
Zunfu Lv ◽  
...  
Keyword(s):  
Ipomoea Batatas ◽  
Sucrose Phosphate Synthase ◽  
Vacuolar Invertase ◽  
Tuberous Roots ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

scholarly journals Cloning, Expression and Genetic Transformation of Sucrose Phosphate Synthase (Sps) Gene in Saccharum Spontaneum L.

2017 ◽  
Vol 59 (2) ◽  
pp. 7-15
Author(s):  
Yun-Wei Zhang ◽  
Yun-Zhuan Zhou ◽  
Hai-Bo Lu ◽  
Deng-Yu Zheng ◽  
Yan-Hua Huang
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Saccharum Spontaneum ◽  
Reading Frame ◽  
Acid Protein ◽  
Significant Difference ◽  
Key Enzyme ◽  
Young Leaves ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

AbstractSucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.

The Role of Sucrose-Phosphate Synthase in the Control of Photosynthate Partitioning in Zea mays Leaves

1997 ◽  
Vol 24 (1) ◽  
pp. 1 ◽  
Author(s):  
John E. Lunn ◽  
Marshall D. Hatch
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Feedback Inhibition ◽  
Sucrose Phosphate Synthase ◽  
Sucrose Content ◽  
Short Term ◽  
Maize Leaves ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

The influence of light and leaf sucrose content on partitioning of photosynthate and sucrose-phosphate synthase (SPS) activity in maize (Zea mays L.) leaves was investigated. The ratio of partitioning of photosynthate between sucrose and starch shifted from about 17:1 to 2:1 when the irradiance was increased from 180 to 1450 µmol quanta m-2 s-1. Increasing the sucrose content of the leaves had little effect on the partitioning ratio. SPS from illuminated leaves had a higher affinity for its substrates, UDPGlc and Fru6P, and was less inhibited by Pi than the enzyme from darkened leaves but the Vmax was unaffected. SPS was fully light activated at an irradiance of 340 but not 180 µmol quanta m-2 s-1. Increasing the sucrose content of maize leaves more than 3-fold had little or no effect on the activation state of SPS which, together with the partitioning data, suggests that sucrose does not exert significant short-term feedback inhibition of its own synthesis in this species.

Sign in / Sign up

Export Citation Format

Share Document