Genetic male sterility breeding in heat tolerant bell pepper: Introgression of ms10 gene from hot pepper through marker-assisted backcrossing

Recurrent parent phenome recovery analysis in six different populations with the introgression of genetic male sterile gene ms10 from hot pepper to heat tolerant bell pepper

10.21203/rs.3.rs-643758/v1 ◽

2021 ◽

Author(s):

Menisha Rani ◽

Salesh Kumar Jindal ◽

Om Prakash Meena

Keyword(s):

Fruit Weight ◽

Bell Pepper ◽

Hot Pepper ◽

Recurrent Parent ◽

Male Sterile ◽

Breeding Method ◽

Cross Combination ◽

Successful Recovery ◽

Backcross Breeding ◽

Heat Tolerant

Abstract Backcrossing is one of the most commonly used breeding methods to transfer target trait(s) into a bell pepper inbreds. We attempted for the first time to transfer genetic male sterile (GMS) ms10 gene from the donor parent MS-12, popular GMS line in India, into the genetic background of six heat tolerant bell pepper inbreds, in restricted generations through the marker-assisted selection (MAS) and backcross breeding method, to develop new GMS lines in bell pepper. In this project, the recurrent parent phenome (RPP) recovery was analyzed in each generations of backcrossing using economic phenotypic markers. Phenotypic background analysis revealed that the extent of RPP recovery ranged from 43.59% (MSSM-1) to 66.26% (MSSM-21) and 70.89% (MSSM-17) to 92.09% (MSSM-21) for fruit weight, from 19.0% (MSSM-21) to 55.14% (MSSM-3) and 61.0% (MSSM-21) to 98.75% (MSSM-1) for number of lobes fruit− 1, from 55.77% (MSSM-17) to 94.25% (MSSM-2) and 69.83% (MSSM-17) to 98.26% (MSSM-3) for total fruit yield plant− 1 in BC1F1 and BC2F1 populations, respectively. Based on a paired 't' test analysis in BC2F1 population, out of six, the MSSM-1 and MSSM-2 generation was shown to be non-significant for all 19 studied traits followed by MSSM-3 (18), indicating a successful recovery of the recipient genome through marker-assisted backcross breeding after successful introgression of the ms10 gene. The GMS lines developed from this project will be utilized in bell pepper breeding programs aimed to identify the best cross-combination for their cultivation under north-Indian plains.

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Heat-tolerant hot pepper exhibits constant photosynthesis via increased transpiration rate, high proline content and fast recovery in heat stress condition

Scientific Reports ◽

10.1038/s41598-021-93697-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sherzod Nigmatullayevich Rajametov ◽

Eun Young Yang ◽

Myeong Cheoul Cho ◽

Soo Young Chae ◽

Hyo Bong Jeong ◽

...

Keyword(s):

Heat Treatment ◽

Heat Stress ◽

Heat Tolerance ◽

Transpiration Rate ◽

Stress Condition ◽

Proline Content ◽

Hot Pepper ◽

Fast Recovery ◽

Heat Stress Condition ◽

Heat Tolerant

AbstractUnderstanding the mechanism for heat tolerance is important for the hot pepper breeding program to develop heat-tolerant cultivars in changing climate. This study was conducted to investigate physiological and biochemical parameters related to heat tolerance and to determine leaf heat damage levels critical for selecting heat-tolerant genotypes. Seedlings of two commercial cultivars, heat-tolerant ‘NW Bigarim’ (NB) and susceptible ‘Chyung Yang’ (CY), were grown in 42 °C for ten days. Photosynthesis, electrolyte conductivity, proline content were measured among seedlings during heat treatment. Photosynthetic rate was significantly reduced in ‘CY’ but not in ‘NB’ seedlings in 42 °C. Stomatal conductivity and transpiration rate was significantly higher in ‘NB’ than ‘CY’. Proline content was also significantly higher in ‘NB’. After heat treatment, leaf heat damages were determined as 0, 25, 50 and 75% and plants with different leaf heat damages were moved to a glasshouse (30–32/22–24 °C in day/night). The growth and developmental parameters were investigated until 70 days. ‘NB’ was significantly affected by leaf heat damages only in fruit yield while ‘CY’ was in fruit set, number and yield. ‘NB’ showed fast recovery after heat stress compared to ‘CY’. These results suggest that constant photosynthetic rate via increased transpiration rate as well as high proline content in heat stress condition confer faster recovery from heat damage of heat-tolerant cultivars in seedlings stages.

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Molecular mapping of a thermosensitive genetic male sterility gene in rice using bulked segregant analysis

Genome ◽

10.1139/g97-027 ◽

1997 ◽

Vol 40 (2) ◽

pp. 188-194 ◽

Cited By ~ 59

Author(s):

P. K. Subudhi ◽

R. P. Borkakati ◽

S. S. Virmani ◽

N. Huang

Keyword(s):

Male Sterility ◽

Molecular Mapping ◽

Bulked Segregant Analysis ◽

Pcr Primers ◽

Rflp Markers ◽

Arbitrary Primers ◽

Repulsion Phase ◽

Genetic Male Sterility ◽

True Breeding ◽

Efficient Alternative

The thermosensitive genetic male sterility (TGMS) system is considered to be a more efficient alternative to the cytoplasmic male sterility (CMS) system for hybrid rice. An F2 population from a cross between a TGMS mutant line (IR32364TGMS) and IR68 was used to map the TGMS gene tms3(t). Fertile and sterile bulks were constructed following the classification of F2 plants into true breeding sterile, fertile, and segregating fertile plants based on F3 family studies. From the survey of 389 arbitrary primers in bulked segregant analysis, four RAPD markers were identified in which three, OPF182600, OPB19750, and OPAA7550, were linked to tms3(t) in repulsion phase and one, OPAC3640, was linked to tms3(t) in coupling phase. The tms3(t) gene was flanked by OPF182600 and OPAC3640 on one side and by OPAA7550 and OPB19750 on the other side. All four markers were low-copy sequences and two of them (OPF182600 and OPAC3640) detected polymorphism when the markers were used to probe the genomic blots. Subsequently, OPAC3640 was mapped to the short arm of chromosome 6 using a mapping population available at IRRI. However, no RFLP markers from this region showed linkage to tms3(t) owing to the lack of polymorphism between the parents. All RAPD fragments were cloned and partially sequenced from both ends. Thus, PCR primers can be designed to develop PCR markers for marker-assisted breeding to facilitate the transfer of tms3(t) from one genetic background to another.Key words: bulked segregant analysis, gene tagging, marker-assisted selection, RAPD, TGMS.

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Heat-tolerant Hot Pepper Exhibits Constant Photosynthesis Via Increased 4 Transpiration Rate, High Proline Content and Fast Recovery in Heat Stress 5 Condition

10.20944/preprints202107.0348.v1 ◽

2021 ◽

Author(s):

Sherzod Rajametov ◽

Eun Young Yang ◽

Myeong Cheoul Cho ◽

Soo Young Chae ◽

Hyo Bong Jeong ◽

...

Keyword(s):

Heat Treatment ◽

Heat Stress ◽

Photosynthetic Rate ◽

Heat Tolerance ◽

Transpiration Rate ◽

Proline Content ◽

Hot Pepper ◽

Fast Recovery ◽

Heat Damage ◽

Heat Tolerant

Understanding the mechanism for heat tolerance is important for the hot pepper breeding program to develop heat-tolerant cultivars in changing climate. This study was conducted to investigate physiological and biochemical parameters related to heat tolerance and to determine leaf heat damage levels critical for selecting heat-tolerant genotypes. Seedlings of two commercial cultivars, heat-tolerant ‘NW Bigarim’ (NB) and susceptible ‘Chyung Yang’ (CY), were grown in 42 °C for ten days. Photosynthesis, electrolyte conductivity, proline content were measured among seedlings during heat treatment. Photosynthetic rate was significantly reduced in ‘CY’ but not in ‘NB’ seedlings in 42 °C. Stomatal conductivity and transpiration rate was significantly higher in ‘NB’ than ‘CY’. Proline content was also significantly higher in ‘NB’. After heat treatment, leaf heat damages were determined as 0, 25, 50 and 75% and plants with different leaf heat damages were moved to a glasshouse (30–32/22–24 °C in day/night). The growth and developmental parameters were investigated until 70 days. ‘NB’ was significantly affected by leaf heat damages only in fruit yield while ‘CY’ was in fruit set, number and yield. ‘NB’ showed fast recovery after heat stress compared to ‘CY’. These results suggest that constant photosynthetic rate via increased transpiration rate as well as high proline content in heat stress condition confer faster recovery from heat damage of heat-tolerant cultivars in seedlings stages.

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